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Method for identifying sashimi of thunnus obesus, thunnus alalunga and thunnus thynnus

A bluefin tuna and identification method technology, applied in the field of food testing, can solve the problems of difficulty in identifying the authenticity of source fish species, save time and cost, combat counterfeiting and shoddy behavior, and maintain market operation order.

Inactive Publication Date: 2012-10-17
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a method for identification of source fish species of bigeye, albacore, and bluefin tuna sashimi, aiming at counterfeiting bluefin tuna sashimi and bigeye tuna sashimi on the market. The behavior of tuna sashimi solves the problem that it is difficult to identify the authenticity of the source fish of tuna sashimi

Method used

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  • Method for identifying sashimi of thunnus obesus, thunnus alalunga and thunnus thynnus
  • Method for identifying sashimi of thunnus obesus, thunnus alalunga and thunnus thynnus
  • Method for identifying sashimi of thunnus obesus, thunnus alalunga and thunnus thynnus

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Embodiment Construction

[0034] The technical solution of the present invention will be further explained below in conjunction with the examples.

[0035] For the sashimi (purchased from Shandong Zhonglu Yuanyang (Yantai) Food Co., Ltd.) of three kinds of big eye, albacore and bluefin tuna, adopt the technical method of the present invention to identify.

[0036] 1. DNA extraction of sashimi:

[0037] Extract the DNA of three kinds of sashimi and mark them. The specific operation steps are as follows:

[0038] 1) Wear disposable medical film gloves, wash the surface of sashimi with sterilized deionized water, cut 0.05g muscle (about half the size of mung bean) with sterilized ophthalmologist, cut it into pieces as much as possible, and put it into a 1.5mL sterilized centrifuge tube , add 0.5mL of cell lysate (10mM Tris-cl, 0.1M EDTA, pH=8.0; 0.5% (m / v) SDS), then add proteinase K with a final concentration of 100μg / mL, vortex to mix, In a water bath at 50°C, mix gently every 15 minutes until the mus...

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Abstract

The invention provides a method for identifying sashimi of thunnus obesus, thunnus alalunga and thunnus thynnus, belonging to the technical field of food detection. The method comprises the following steps: extracting thunnus sashimi DNAs (deoxyribonucleic acid), taking the extracted DNAs as templates and F1 / F2 as a primer, carrying out PCR (polymerase chain reaction) amplification on cytb (cytochrome) gene fragments, carrying out electrophoresis detection on the amplified fragments, carrying out enzyme digestion on the purified PCR products with restriction enzymes Fok I and Stu I, and analyzing a restriction map through agarose gel electrophoresis to identify authenticity of raw fish of the three kinds of thunnus sashimi. By adopting the method, the source fish varieties of thunnus sashimi are identified on a molecular level and the sources of thunnus sashimi can be accurately differentiated. Thus, the method has great significance in standardizing the thunnus sashimi markets.

Description

Technical field: [0001] The invention belongs to the technical field of food detection, and in particular relates to an identification method for sashimi of large order, albacore and bluefin tuna. Background technique: [0002] In terms of biological classification, tuna refers to a total of 8 species of fish in the genus Thunnus of the family Scombridae of the order Perciformes. Species with high economic value include bluefin tuna (Thunnus maccoyii), bigeye tuna (Thunnus obesus), yellowfin tuna (Thunnus albacares), albacares (Thunnus alalunga), etc. Among them, bigeye tuna, albacore, bluefin tuna Fin tuna is the main raw material fish for domestic sashimi. [0003] Tuna is one of the three most nutritious fish recommended by the International Nutrition Society. It has tender meat, high protein and low fat, and is rich in various vitamins and trace elements, especially unsaturated fatty acids such as DHA and EPA. DHA is known as "Brain gold" is an essential nutrient for t...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 姚琳王联珠江艳华李青娇朱文嘉翟毓秀宋春丽李晓庆
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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