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New method for purifying fructo oligosaccharide in chicory

A technology of fructooligosaccharides and chicory, applied in the field of fructooligosaccharides, can solve the problems of low extraction purity and low extraction rate, and achieve the effects of increasing extraction speed, increasing the content of active ingredients, saving medicinal materials and energy consumption

Active Publication Date: 2012-10-17
DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the shortcomings of conventional techniques such as relatively low extraction rate and low extraction purity, and provide a combination of biological enzyme-microwave extraction technology, macroporous resin decolorization and impurity removal, and ultrafiltration and nanofiltration combined with purification of chicory. Polyfructo method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: a kind of utilizing Aspergillus niger endo-inuclease-microwave extraction combination technology, macroporous resin decolorization and impurity removal, ultrafiltration, nanofiltration combined with the method of purifying fructooligosaccharides in chicory, its steps are as follows:

[0016] (1) 10 kilograms of chicory are broken into 30 orders, adjusted into an aqueous solution with a concentration of about 15%, then add Aspergillus niger endo-inulinase, the amount of endo-inulinase added is 10U / g inulin, and the pH value is adjusted at 4.8, enzymatically hydrolyze at about 40°C for 1 hour, and filter to obtain the filtrate;

[0017] (2) The filtrate is extracted under the conditions of microwave power 300W, frequency 4000MHz, temperature 60°C, time 100S;

[0018] (3) The extract is filtered, decolorized with a polar macroporous resin, the model is GDX-501, and the fructooligosaccharide solution is collected;

[0019] (4) The fructooligosaccharide soluti...

Embodiment example 2

[0023] Implementation case 2: a method utilizing Aspergillus niger inulin endoenzyme-microwave extraction combined technology, macroporous resin decolorization and impurity removal, ultrafiltration and nanofiltration in combination with purification of fructooligosaccharides in chicory, the steps are as follows:

[0024] (1) 10 kilograms of chicory are broken into 40 orders, adjusted into an aqueous solution with a concentration of about 20%, then add Aspergillus fugus endo-inulinase, the amount of endo-inulinase added is 10U / g inulin, and the pH value is adjusted at 5.0, enzymatically hydrolyze at about 45°C for 1.2h, and filter to obtain the filtrate;

[0025] (2) The filtrate is extracted under the conditions of microwave power 400W, frequency 6000MHz, temperature 65°C, and time 200S;

[0026] (3) The extract is filtered, decolorized with a weakly polar macroporous resin, the model is AB-8, and the fructooligosaccharide solution is collected;

[0027] (4) The fructooligosa...

Embodiment example 3

[0031] Implementation case 3: a method utilizing Aspergillus niger inulin endo-inuclease-microwave extraction combined technology, macroporous resin decolorization and impurity removal, ultrafiltration and nanofiltration in combination with purification of fructooligosaccharides in chicory, the steps are as follows:

[0032] (1) 10 kilograms of chicory are broken into 50 orders, adjusted into an aqueous solution with a concentration of about 30%, then add Aspergillus niger endo-inulinase, the amount of endo-inulinase added is 10U / g inulin, and the pH value is adjusted at 5.5, enzymatically hydrolyze at about 50°C for 1.5h, and filter to obtain the filtrate;

[0033] (2) The filtrate is extracted under the conditions of microwave power 500W, frequency 10000MHz, temperature 70°C, time 300S;

[0034] (3) Filter the extract, decolorize and remove impurities with a polar macroporous resin, the model is GDX-501, and collect the fructooligosaccharide solution;

[0035] (4) The fruct...

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PUM

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Abstract

The invention belongs to the field of natural organic chemistry, and relates to a method for purifying fructo oligosaccharide in chicory by using an Aspergillus niger endo-inulinase-microwave extraction combined technology, macroporous resin decolorization and impurity removing, and combination of ultrafiltration and nanofiltration. The method has the following advantages that: 1, the Aspergillus niger endo-inulinase-microwave extraction combined technology is adopted so as to substantially improve the yield of the fructo oligosaccharide, greatly reduce the extraction time, and improve the extraction speed; the macroporous resin is selected so as to substantially improve the active ingredient content, and achieve the large-scale production, wherein the macroporous resin provides a large absorption amount for pigment, and the inulin loss is low during the decolorization process; and 3, the ultrafiltration and nanofiltration equipment is adopted, such that the extraction purity of the fructo oligosaccharide is high, the separation effect is significant, and the permeation flux of the membrane can be restored after the membrane is washed. By combining the Aspergillus niger endo-inulinase-microwave extraction combined technology, the macroporous resin decolorization and impurity removing, and the ultrafiltration and nanofiltration filtration process, the final product fructo oligosaccharide with the content more than 98% can be obtained. With the present invention, disadvantages of long time, existing of impurities, and high active ingredient loss of the conventional inulin extraction method are overcome.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for utilizing Aspergillus niger endo-inulinase-microwave extraction combined technology, macroporous resin decolorization and impurity removal, and ultrafiltration and nanofiltration combined to purify fructooligosaccharides in chicory. Background technique: [0002] Chicory: a perennial herb of Compositae, which can be used not only for medicine, but also as an excellent feed crop and high-grade vegetable. It has important physiological functions and high health value. It is distributed both at home and abroad. Chicory is a dual-purpose plant for medicine and food. Chicory leaves can be used to prepare lettuce. It has been introduced and cultivated in Sichuan (Chengdu) and Guangdong in my country. Its root contains inulin and aromatic substances, which can be used to extract substitute coffee and promote the activities of human digestive organs. The aerial part...

Claims

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Application Information

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IPC IPC(8): C12P19/14C08B37/18
Inventor 姚德坤姚德利张亚红万莉
Owner DAXINGANLING LINGOBERRY BOREAL BIOTECH CO LTD
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