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Small G protein Rop (Rho-related GTPase from plant) family protein of Hevea brasiliensis latex and coding gene thereof

A technology encoding genes and proteins, applied in genetic engineering, plant genetic improvement, angiosperms/flowering plants, etc., can solve the problems of no in-depth research, no cloned member genes, etc. The effect of radial development

Inactive Publication Date: 2012-10-17
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

(Li, D., et al., Identification and characterization of genes associated with tapping panel dryness from Hevea brasiliensis latex using suppression subtractive hybridization. BMC Plant Biol, 2010.10: p.140.), they only reported the partial sequence of the Rop member , did not clone the gene of this member, and did not conduct more in-depth research, but their research is enough to prove the correctness of our speculation

Method used

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  • Small G protein Rop (Rho-related GTPase from plant) family protein of Hevea brasiliensis latex and coding gene thereof
  • Small G protein Rop (Rho-related GTPase from plant) family protein of Hevea brasiliensis latex and coding gene thereof
  • Small G protein Rop (Rho-related GTPase from plant) family protein of Hevea brasiliensis latex and coding gene thereof

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Embodiment 1

[0035] Example 1. Acquisition and effect verification of the small G protein Rop member HbRop5 encoding gene

[0036] For the first time, we cloned the full-length cDNA of HbRop5, a member of the small G protein Rop family in Hevea latex, and performed sequence analysis and functional studies.

[0037] 1. Acquisition of small G protein Rop member protein and its coding gene HbRop5

[0038] We established the rubber tree latex EST sequence database, found the EST fragment of the small G protein Rop member coding gene HbRop5, and further used the rapid amplification of cDNA ends (3'RACE) to finally obtain the cDNA sequence containing the complete reading frame.

[0039] The specific method is as follows:

[0040] Obtained conservative fragment

[0041] By searching the annotated Hevea latex EST sequence database we established, we found a 399bp EST fragment of the gene HbRop5 encoding the small G protein Rop member. According to Genetyx software analysis, the EST fragment has ...

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Abstract

The invention discloses a coding gene of small G protein Rop (Rho-related GTPase from plant) and application. The protein has one of the following amino acid residual sequences: 1) amino acid residual sequence of SEQ ID NO.1 in a sequence table; and 2) protein which has an amino acid residual sequence of SEQ ID NO.1 in the sequence table by substitution and / or loss and / or addition of one or more amino acid residues, has the GTP (Guanosine Triphosphate) binding function and is derived from SEQ ID NO.1. The protein has GTP binding activity, can regulate synthesis and metabolization of the active gron ROS, praticifate seuv, participates in stress response, has the characteristics of regulating development of the secondary xylem and phloem of woods, and can be used for improving the resistance of the protein in an adverse situation in genetically modified crops or woods, so that the yield can be increased.

Description

technical field [0001] The invention relates to a rubber tree latex small G protein Rop family member protein, its coding gene and its application. Background technique [0002] The small G protein is a monomeric protein that binds to guanylic acid, and acts as a molecular switch in eukaryotic cells (Zhenbiao Yang, Small GTPases: Versatile Signaling Switches in Plants. The Plant Cell, 2002.14, p.S375-S388; V Vernoud , V., et al., Analysis of the small GTPase gene superfamily of Arabidopsis. Plant physiology, 2003.131 (3): p.1191-1208; Nibaua, C., Wu, HM. and Cheung, AY., RAC / ROP GTPases: hubs' for signal integration and diversification in plants. Trends in Plant Science, 11(6): p.309-315.). Many studies have confirmed that members of the plant small G protein Rop subfamily are a unique class of genes formed in plant evolution, and they are widely involved in various physiological activities in plant cells, including the organization of actin cytoskeleton, cell polarity, an...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21A01H5/00
Inventor 秦云霞阳江华黄亚成王岳坤龙翔宇戚继艳唐朝荣
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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