Method for separating high-purity succinic acid from fermentation broth

A succinic acid, high-purity technology, applied in the field of separation of high-purity succinic acid, can solve the problems of cumbersome operation and large investment, and achieve the effect of simplifying the process flow, good product safety, and saving microfiltration and ultrafiltration units

Active Publication Date: 2012-10-17
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there is no need to add organic solvent or activated carbon in this process, the investment in the process equipment is relatively large and the operation is cumbersome.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036]Take 4.0L of anaerobic fermentation broth with a succinic acid concentration of 37.6g / L, and centrifuge to remove bacteria and large particle suspensions in a centrifuge. The centrifuged supernatant obtained was evaporated to 3.2 L of water (concentrated 5 times) at a temperature of 80°C and a pressure of 0.04 Mpa. Centrifuge the denatured protein in a centrifuge to obtain 0.7 L of distilled liquid, wash the denatured protein with 0.014 L of deionized water to remove a small amount of succinic acid adsorbed, and combine water for washing the denatured protein with the distilled liquid to form 0.71 L of concentrated liquid. Add 0.014Kg of activated carbon to the above-mentioned concentrated liquid, and decolorize and remove impurities for 30 minutes at a temperature of 20°C. Remove the activated carbon by filtration, and add 0.04 L of deionized water to rinse the activated carbon. After washing, the deionized water and the decolorized, impurity-removed and filtered liquid...

Embodiment 2

[0040] Take 4.0L of anaerobic fermentation broth with a succinic acid concentration of 37.6g / L, and centrifuge to remove bacteria and large particle suspensions in a centrifuge. The centrifuged supernatant obtained was evaporated to 3.2 L of water (concentrated 5 times) at a temperature of 145°C and a pressure of 0.10 MPa. Centrifuge the denatured protein in a centrifuge to obtain 0.7 L of distilled liquid, wash the denatured protein with 0.056 L of deionized water to remove a small amount of succinic acid adsorbed, and combine water for washing the denatured protein with the distilled liquid to form 0.75 L of concentrated liquid. Add 0.05Kg of activated carbon to the above-mentioned concentrated liquid, and decolorize and remove impurities at a temperature of 55° C. for 30 minutes. Remove the activated carbon by filtration, and add 0.04 L of deionized water to rinse the activated carbon. After washing, the deionized water and the decolorized, impurity-removed and filtered liq...

Embodiment 3

[0044] Get 4.0L of anaerobic fermentation broth whose succinic acid concentration is 46.2g / L, and centrifuge on a centrifuge to remove bacteria and large particle suspensions. The centrifuged supernatant obtained was evaporated to 3.4 L of water (concentrated 6.7 times) at a temperature of 120°C and a pressure of 0.09 Mpa. Centrifuge the denatured protein on a centrifuge to obtain 0.5 L of distilled liquid, wash the denatured protein with 0.04 L of deionized water to remove a small amount of succinic acid adsorbed, and combine water for washing the denatured protein with the distilled liquid to form 0.54 L of concentrated liquid. Add 0.04Kg of activated carbon to the above-mentioned concentrated liquid, and decolorize and remove impurities at a temperature of 70° C. for 30 minutes. Remove the activated carbon by filtration, and add 0.04 L of deionized water to rinse the activated carbon. After washing, the deionized water and the decolorized, impurity-removed and filtered liqu...

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Abstract

The invention provides a method for separating high-purity succinic acid from a fermentation broth, comprising the following steps of: removing thalline from a succinic acid fermentation broth, condensing, centrifuging, adding active carbon to remove impurities, crystallizing under an acidic condition, filtering, and drying to obtain high-purity succinic acid. The invention is superior to the prior art in the following aspects: the combination of protein denaturation and dehydration is realized through distillation, the denatured protein can be removed from a concentrate in a simple way, microfiltration and ultrafiltration units are saved, the technological flow is simplified, the equipment investment cost is reduced, and there exist no discharge of microfilt membrane-washing wastewater after washing of microfiltration and ultrafilter membranes.

Description

1. Technical field [0001] The invention belongs to the field of biochemical separation, and in particular relates to a method for separating high-purity succinic acid from fermentation broth. 2. Background technology [0002] Succinic acid, also known as succinic acid, is one of the most important organic dibasic acids in C4 platform compounds, and can be widely used in polyester, rubber, pharmaceutical, food industry, protective coatings, dyes and other fine in the chemical industry. At present, the synthesis of succinic acid mainly adopts the chemical electrolysis method of butane via maleic anhydride. This method is polluting and costly, which seriously hinders the large-scale production of succinic acid. Compared with the chemical electrolysis method, the preparation of succinic acid by the biological fermentation method has the characteristics of using cheap renewable resources as raw materials and fixing the greenhouse gas carbon dioxide to achieve emission reduction....

Claims

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Application Information

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IPC IPC(8): C07C55/10C07C51/43C07C51/47
Inventor 刘经纬阚林陈韶辉李晓强李泽壮柏基业杨爱武刘丽娟孟海朱伟
Owner CHINA PETROLEUM & CHEM CORP
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