Construction and preparation of three-dimensional bionic electropolarized gradient pore nerve conduit

Abstract

The invention provides construction and preparation of a three-dimensional bionic electropolarized gradient pore nerve conduit. The nerve conduit is an electropolarized beta-tricalcium phosphate / polylactic acid / hyaluronic acid / chitosan / nerve growth factor nerve conduit, and is prepared by the steps of: preparing a uniformly dispersed electropolarized beta-tricalcium phosphate and polylactic acid core material by using polylactic acid as a substrate material and adding the electropolarized beta-tricalcium phosphate; and preparing a three-dimensional bionic electropolarized gradient pore nerve conduit with component and pore structure double gradient by attaching the hyaluronic acid, the chitosan and the nerve growth factor to the surface of the core material by virtue of electrostatic self-assembly and coupling crosslinking, wherein the length is between 10 and 100mm, the inner diameter is between 1 and 6mm, the wall thickness is between 0.5 and 2mm, and the outer tube wall is of a three-dimensional network structure. The nerve conduit is applied to repairing of a peripheral nervous system, induction of peripheral nerve regeneration and in-vitro culture of nerve cells in tissue engineering. The three-dimensional bionic electropolarized gradient pore nerve conduit has the advantages of simple preparation method, low cost, environmental friendliness and high economical benefit, and is applicable to industrial production.

Description

technical field [0001] The invention belongs to the field of nerve conduit construction and preparation thereof, in particular to the construction and preparation of a three-dimensional bionic electric polarization gradient hole nerve conduit. Background technique [0002] Peripheral nerve defect is a common clinical disease, about 2.8% of trauma patients lead to peripheral nerve injury, and severe cases will be permanently disabled. Hundreds of thousands of cases in my country need nerve repair every year, but the repair effect is not satisfactory, so the regeneration and functional recovery of peripheral nerve injury has always been a hot topic in the field of neuroscience. At present, autologous nerve transplantation, as the gold standard for peripheral nerve injury, is the most classic method of bridging and repairing nerve defects, but it is not the most ideal method. In addition to the limited source of donors, it will cause partial function loss of the donor site ...

AI Technical Summary

Problems solved by technology

However, any single degradable material cannot meet the above requirements. Only by making full use of the biologically active elements of natural polymers and the good mechanical processing properties of synthetic polymers, facilitating mass production and low cost, can natural polymers and Synthetic polymer structure hybridization and complementary advantages can make the peripheral nerve regeneration catheter prepared with more performance

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