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Method for rapidly propagating small leopard amanita mycelium

A technology of mycelium and amanita, which is applied in the field of rapid indoor cultivation of wild poisonous fungus Amanita amanita mycelium, can solve the problems of slow growth of mycelium in the early stage and difficulty in multiplying, so as to shorten the growth cycle and reduce production costs. Low, easy-to-operate effect

Inactive Publication Date: 2012-10-17
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the mycelia of this species have been successfully isolated, but the early growth stage of the mycelia is still very slow, and it is difficult to multiply. The present invention has made a key breakthrough on this problem.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0020] Pick young fruiting bodies with good growth, no pests, and unopened umbrellas in the field; bring the fruiting bodies back to the laboratory, and use alcohol cotton balls on the ultra-clean table to gently wipe the surface of the cap and the stipe;

[0021] Slightly remove the epidermis and the surrounding part of the cap with a sterile blade, and cut the remaining middle part of the cap and the stipe into pieces of about 0.10-0.25cm 2 Small squares, gently embedded in the surface of the induction medium, the medium is modified SPDM medium (potato 200.00g / L, glucose 20.00g / L, MgSO 4 1.00g / L, CaCl 2 1.00g / L, KH 2 PO 4 0.50g / L, NH 4 NO 3 0.35g / L, KNO 3 0.35g / L, vitamin B 1 0.10mg / L, agar 12.00g / L), add wort juice (15.0 Baume) 150ml, control the pH value to 5.4, culture temperature is 22℃, culture in dark for 40 days, the tissue blocks are raised in many places, and the surface grows lumps , dense fluffy white hyphae;

[0022] Pick out the well-growing myceli...

example 2

[0024] Pick young fruiting bodies with good growth, no pests, and unopened umbrellas in the field; bring the fruiting bodies back to the laboratory, and use alcohol cotton balls on the ultra-clean table to gently wipe the surface of the cap and the stipe;

[0025] Slightly remove the epidermis and the surrounding part of the cap with a sterile blade, and cut the remaining middle part of the cap and the stipe into pieces of about 0.10-0.25cm 2 Small squares, gently embedded in the surface of the induction medium, the medium is modified SPDM medium (potato 200.00g / L, glucose 20.00g / L, MgSO 4 1.00g / L, CaCl 2 1.00g / L, KH 2 PO 4 0.50g / L, NH 4 NO 3 0.35g / L, KNO 3 0.35g / L, vitamin B1 0.10mg / L, agar 12.00g / L), supplemented wort juice (15.5 Baume) 165ml, controlled PH value to 5.6, culture temperature was 23°C, cultured in dark for 42 days, the tissue pieces bulged in many places, and the surface grew lumps , dense fluffy white hyphae;

[0026] Pick out the well-growing my...

example 3

[0028] Pick young fruiting bodies with good growth, no pests, and unopened umbrellas in the field; bring the fruiting bodies back to the laboratory, and use alcohol cotton balls on the ultra-clean table to gently wipe the surface of the cap and the stipe;

[0029] Slightly remove the epidermis and the surrounding part of the cap with a sterile blade, and cut the remaining middle part of the cap and the stipe into pieces of about 0.10-0.25cm 2 Small squares, gently embedded in the surface of the induction medium, the medium is modified SPDM medium (potato 200.00g / L, glucose 20.00g / L, MgSO 4 1.00g / L, CaCl 2 1.00g / L, KH 2 PO 4 0.50g / L, NH 4 NO 3 0.35g / L, KNO 3 0.35g / L, vitamin B 1 0.10mg / L, agar 12.00g / L), add wort juice (16.0 Baume) 180ml, control PH value to 5.4, culture temperature is 24°C, culture in dark for 38 days, the tissue blocks bulge in many places, and the surface grows lumps , dense fluffy white hyphae;

[0030] Pick out the well-growing mycelia in the...

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PUM

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Abstract

The invention discloses a method for rapidly propagating wild toadstool small leopard amanita mycelium indoor, and belongs to the technical field of cultivation of macrofungi. The method is characterized in that malt extract, glutamic acid and active substances for growth are simply added in young sporocarp mediotrastum induced mycelium before depucelation so that the mycelium can be subjected to rapid mass propagation. According to the invention, a simple culture medium is adopted, the cultivation period is short, the cost is low, and solid culture of the small leopard amanita mycelium can be realized on a large scale. The genus amanita is a large economic fungi which is special and precious and has great value and a wide application range in potentially developing new specific medicines such as anti-tumor medicines, antibacterial and antiviral medicines, sedatives, and narcotics; however, the genus amanita is difficult to develop and apply because the problems that resource of the genus amanita is rare, the genus amanita is difficult to artificially domesticate and the chemical synthetic of toxin is inactive. Aiming to the problems that the amanita mycelium is difficult to induce and grows slowly, the invention brings an innovative breakthrough and provides conditions to large-scale culture and artificial domestication of mycelium and the like.

Description

technical field [0001] The invention relates to a method for rapid indoor cultivation of the mycelium of wild poisonous fungus Amanita chinensis, which belongs to the field of biotechnology, and specifically belongs to the category of indoor cultivation of large poisonous fungi. Background technique [0002] Amanita ( Amanita ) belongs to Basidiomycotina, Phytomycetes, Agaricales, Amanitaceae ( Amanitaceae ), is a more special and valuable worldwide widespread genus among poisonous macrofungi, and its species diversity is very rich. Nearly 400 species have been reported in the world, and nearly 100 species (including subspecies, variants and variants) have been recorded in China. According to research, there are still many species in China that have not yet been studied and named. However, with the emergence of the global ecological crisis, many large fungal resources in our country are in danger, in a serious decline and endangered state, and some species are facing the...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 李宗菊曹杰张曦予刘弟王鹏飞何隽李彪周文吴鹏
Owner YUNNAN UNIV
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