Detection kit for helicobacter pylori virulence protein antibody and detection method by using the same

Abstract

The invention relates to a detection kit for helicobacter pylori virulence protein antibody and a detection method by using the same. The kit comprises a microwell plate, an enzyme-labeled secondary antibody, a negative control serum, a positive control serum, a substrate, a stop buffer and a washing liquor, wherein the microwell plate is coated with purified recombinant helicobacter pylori virulence proteins, the substrate is a solution containing 1% m / m 3, 3', 5, 5'-tetramethyl benzidine and 30% v / v hydrogen peroxide, and the recombinant helicobacter pylori virulence proteins are cytotoxin-associated protein CagA, vacuolating toxin protein VacA, flagellum protein subunit A, flagellum protein subunit B, urease subunit A, and urease subunit B. According to the invention, six purified helicobacter pylori virulence proteins are used as antigens which itself have high conservatism and specificity and are related to the symptom of a patient, so the kit has characteristics of high specificity, identification capacity for helicobacter pylori clinical strain virulence strength, and semi-quantitative detection capacity.

Description

technical field [0001] The invention relates to the fields of enzyme-linked immunosorbent adsorption and clinical diagnosis of Helicobacter pylori, in particular to a detection kit for the virulence protein antibody of Helicobacter pylori and a detection method thereof. Background technique [0002] Helicobacter pylori (Hp), discovered by Australian doctors in 1983, is a spiral-shaped, Gram-negative bacterium that colonizes the human stomach. Helicobacter pylori infection is an important cause of human gastrointestinal diseases, which can lead to chronic gastritis, severe atrophic gastritis, peptic ulcer and gastric cancer. 30-50% of the population in western countries has been infected with Helicobacter pylori, and the infection rate of Helicobacter pylori in my country is as high as 70-90%. With the increase of age, the infection rate tends to increase. 10% of infected patients will develop clinical symptoms, including gastritis, gastroduodenal ulcer, etc. If they do not ...

AI Technical Summary

Problems solved by technology

However, the Helicobacter pylori antigen involved in the test is a partially purified whole antigen of Helicobacter pylori, which cannot fully meet the detection requirements in terms of specificity and stability.

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