Preparation method for manufacturing silkworm pupa protein of protein filament
A technology of silkworm chrysalis protein and silkworm chrysalis protein powder, which is applied to the preparation method of peptides, chemical instruments and methods, peptides, etc. It can solve the problems of broken silkworm chrysalis protein fibers, recycling of silkworm chrysalis protein peptides, and affecting the quality of spinning, and achieves the goal of product The effect of high quality, low production cost, and convenience for industrial scale production
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Embodiment 1
[0025] A method for preparing silkworm chrysalis protein for making protein silk, the specific steps of the method are as follows:
[0026] (1) Preparation of defatted silkworm chrysalis powder alkaline solution
[0027] Using commercially available degreased silkworm chrysalis as raw material, the degreased silkworm chrysalis is first sent into a pulverizer for pulverization, then the powder is collected and separated through a 20-mesh sieve, and the sieved powder (that is, the prepared degreased silkworm chrysalis powder) is collected; The crude product is returned to the pulverizer for further pulverization. The defatted silkworm chrysalis powder that collects is sent in the batching kettle again, according to the quality (kg) of defatted silkworm chrysalis powder: the massfraction of sodium hydroxide is that the ratio of the volume (L) of the aqueous solution of 0.1% sodium hydroxide is 1: 8 Ratio, pump the sodium hydroxide aqueous solution into the batching kettle, stir ...
Embodiment 2
[0037] A preparation method of silkworm chrysalis protein for making protein silk, same as embodiment 1, wherein:
[0038] In the (1) step, collect powder and cross 40 mesh sieves to separate, according to the quality (kg) of defatted silkworm chrysalis powder: the massfraction of sodium hydroxide is the ratio of the volume (L) of the aqueous solution of 1% sodium hydroxide is The ratio of 1:12, pump the sodium hydroxide aqueous solution into the batching kettle.
[0039] In step (2), water vapor at a pressure of 0.25 MPa is introduced into the interlayer of the glass-lined reactor to raise the temperature of the defatted silkworm chrysalis powder alkaline solution in the glass-lined reactor to 75° C. and keep it warm for 0.75 h. Continue to feed water vapor under stirring and continue to raise the temperature to 107 ° C, and keep warm for 1 hour before turning off the water vapor. Then, under stirring, the temperature of the mixed solution of the broken silkworm chrysalis gl...
Embodiment 3
[0044] A preparation method of silkworm chrysalis protein for making protein silk, same as embodiment 1, wherein:
[0045] In the (1) step, collect powder and cross 60 mesh sieves to separate, according to the quality (kg) of defatted silkworm chrysalis powder: the massfraction of sodium hydroxide is the ratio of the volume (L) of the aqueous solution of 1% sodium hydroxide is The ratio of 1:16, pump the sodium hydroxide aqueous solution into the batching tank.
[0046] In step (2), water vapor with a pressure of 0.4 MPa is introduced into the interlayer of the glass-lined reactor to raise the temperature of the defatted silkworm chrysalis powder alkaline solution in the glass-lined reactor to 80° C. and keep it warm for 1 hour. Continue to feed water vapor under stirring and continue to raise the temperature to 115°C, and keep the temperature for 1.5h again, then turn off the water vapor. Then, under stirring, the temperature of the mixed solution of the broken silkworm chry...
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