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Method for extraction of mulberry pectin via coupling of N-methyl morpholine oxide and cellulase

A technology of methylmorpholine and cellulase, which is applied in the field of coupling extraction of mulberry pectin with N-methylmorpholine oxide and cellulase, can solve the problems of low pectin extraction efficiency and improve pectin extraction Efficiency, rapid regeneration cycle, and abundant raw materials

Inactive Publication Date: 2012-10-03
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: the purpose of the present invention is to provide a method for the low efficiency of pectin extraction in the prior art N -Methylmorpholine oxide and cellulase coupled method for extracting Mori pectin

Method used

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  • Method for extraction of mulberry pectin via coupling of N-methyl morpholine oxide and cellulase
  • Method for extraction of mulberry pectin via coupling of N-methyl morpholine oxide and cellulase

Examples

Experimental program
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Effect test

Embodiment 1

[0017] N -Methylmorpholine Oxide and Trichoderma reesei Cellulase Coupling to Extract Mori Pectin

[0018] (1) Take the mulberry branch, cut off the mulberry bark with a knife, cut off the phloem, take the mulberry bark, wash away the sediment with tap water, and dry at 60°C for 1-2 hours;

[0019] (2) Take 100g of mulberry bark and add 500g of 85% concentration N - Methylmorpholine oxide (commercially available), treat mulberry bark at 50°C for 10-30 minutes, add 1-3 L of water to the system to precipitate cellulose and other materials;

[0020] (3) Take the upper liquid, add Trichoderma reesei cellulase to a final concentration of 5 FPU / mL, and treat at 45°C for 12 hours to remove cellulose impurities mixed in pectin;

[0021] (4) Add 50 g of activated carbon to the supernatant, mix and decolorize, stir for 10-30 minutes, filter to remove the activated carbon, and obtain the decolorized pectin solution;

[0022] (5) The pectin solution is distilled under reduced pressure ...

Embodiment 2

[0025] N Coupling of -Methylmorpholine Oxide and Aspergillus niger Cellulase to Prepare Mori Pectin

[0026] (1) Take mulberry branches and cut off the mulberry bark with a knife, cut off the phloem, take the mulberry bark, wash away the sediment with tap water, and dry at 60°C for 2 hours;

[0027] (2) Take 100g of mulberry bark and add 200g of 97% N -Methylmorpholine oxide, treat the mulberry bark at 90°C for 10-30 minutes, add 0.75-3 L of water to the system to precipitate materials such as cellulose;

[0028] (3) Take the upper liquid, add Aspergillus niger cellulase to a final concentration of 6 FPU / mL, and treat at 50°C for 5 hours to remove cellulose impurities mixed in pectin;

[0029] (4) Add 50 g of activated carbon to the supernatant, mix and decolorize, stir for 10-30 minutes, filter to remove the activated carbon, and obtain a decolorized pectin solution.

[0030] (5) The pectin solution passes through a 50cm-high column of strong acidic cation exchange resin a...

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Abstract

The invention discloses a method for extraction of mulberry pectin via coupling of N-methyl morpholine oxide and cellulose, which comprises the following steps of: (1) processing mulberry barks with the N-methyl morpholine oxide: using the mulberry barks as raw materials, processing the mulberry barks with the N-methyl morpholine oxide for equal-component separation of pectin and cellulose in the mulberry barks, adding water after processing, so that the pectin is dissolved in water and the cellulose, hemicellulose, lignin and other components are precipitated; (2) processing the cellulase: taking the supernatant liquid, adding the cellulase for processing, and removing cellulose impurities; and (3) preparing the pectin: bleaching the supernatant liquid, adding ethyl alcohol for precipitation of the pectin, washing with absolute ethyl alcohol, and drying and grounding into powder to obtain the mulberry pectin. The N-methyl morpholine oxide is used as a processing agent to extract the pectin in the mulberry barks, the pectin can be extracted efficiently, only 20-50min is needed for processing with the NMMO (N-methyl morpholine oxide), the final yield of pectin can reach 11 percent, and the efficiency of extraction of the pectin from the mulberry barks can be increased.

Description

technical field [0001] The invention belongs to the technical field of biochemical engineering and food engineering, and relates to a N -A method for coupling methylmorpholine oxide and cellulase to extract Mori pectin. Background technique [0002] Pectin is white, light yellow to yellow powder, without fixed melting point and solubility, insoluble in organic solvents such as ethanol. Pectin is a component of plant cell walls, an acidic macromolecular polysaccharide formed by the polymerization of D-galacturonic acid residues through α(1→4) glycosidic bonds. Due to its very good gelling and emulsification stabilization, and being safe and non-toxic, pectin is widely used in food, medicine, cosmetics, etc. as a thickener, stabilizer and emulsifier. The demand for pectin is increasing both at home and abroad, and my country imports a large amount of pectin from abroad every year. Therefore, the development of new sources of pectin and the application of new processes have v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/06
Inventor 李强季更生费娟娟谷绪顶吴再强
Owner JIANGSU UNIV OF SCI & TECH
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