Method for extracting acid-soluble collagen from fish skin and extracted product

A collagen, acid-soluble technology, applied in the direction of animal/human protein, connective tissue peptide, specific peptide, etc., can solve the problem of lack of telopeptide

Active Publication Date: 2013-10-16
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods all use enzyme treatment methods. If only one enzyme, such as pepsin, is added in the extraction process, collagen macromolecules with relatively complete spatial structure and primary structure can also be obtained, but compared with acid-soluble collagen , there will be a lack of telopeptide, that is, the enzyme will slightly degrade

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Embodiment 1: extract acid-soluble collagen from fish skin

[0071] Fresh grass carp is slaughtered and skinned in a cold room, and the residual fish meat on the skin is removed. Then, wash the fish skin with ice water, cut into small pieces and store in the refrigerator for later use.

[0072] The implementation steps are as follows:

[0073] Step A: Remove fish skin impurities

[0074] According to the ratio of fish skin in grams to aqueous sodium hydroxide solution in milliliters: 1:20, the fish skin was treated with 0.05M aqueous sodium hydroxide solution at a temperature of 4° C. for 35 hours to obtain fish skin from which impurities were removed.

[0075] Step B: Remove the skin fat from the fish

[0076] According to the ratio of protein fish skin in grams to fat-removing solution in milliliters: 1:10, the fish skin obtained in step A) was then treated with n-hexane fat-removing solution at a temperature of 4°C for 20 hours to obtain fat-removing fish Skin. ...

Embodiment 2

[0088] Embodiment 2: extract acid-soluble collagen from fish skin

[0089] Fresh carp are slaughtered and skinned in a cold room, and the residual fish meat on the skin is removed. Then, wash the fish skin with ice water, cut into small pieces and store in the refrigerator for later use.

[0090] The implementation steps are as follows:

[0091] Step A: Remove fish skin impurities

[0092] According to the ratio of fish skin in grams to aqueous sodium hydroxide solution in milliliters: 4:35, the fish skin was treated with 0.1 M sodium hydroxide aqueous solution at a temperature of 10° C. for 32 hours to obtain fish skin from which impurities were removed.

[0093] Step B: Remove the skin fat from the fish

[0094]According to the ratio of protein fish skin in grams to degreasing solution in milliliters: 4:16, the fish skin obtained in step A) was then treated with absolute ethanol degreasing solution at a temperature of 10°C for 26 hours to obtain fat-removing fish skin. ...

Embodiment 3

[0106] Embodiment 3: extract acid-soluble collagen from fish skin

[0107] Fresh grass carp is slaughtered and skinned in a cold room, and the residual fish meat on the skin is removed. Then, wash the fish skin with ice water, cut into small pieces and store in the refrigerator for later use.

[0108] The implementation steps are as follows:

[0109] Step A: Remove fish skin impurities

[0110] According to the ratio of fish skin in grams to sodium hydroxide aqueous solution in milliliters: 2:32, the fish skin was treated with 0.2M sodium hydroxide aqueous solution at a temperature of 15° C. for 38 hours to obtain fish skin from which impurities were removed.

[0111] Step B: Remove the skin fat from the fish

[0112] According to the ratio of 2:12 for fish skin with impurities removed in grams and fat-removing solution in milliliters, the fish skin obtained in step A) was then treated with anhydrous ether fat-removing solution at a temperature of 15°C for 24 hours to obtai...

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Abstract

The invention relates to a method for extracting acid-soluble collagen from fish skin and an acid-soluble collagen product extracted by the method. The method comprises the following steps: removing impure protein and fat of the fish skin; extracting the acid-soluble collagen, dialyzing, performing freeze-drying and the like. The acid-soluble collagen product extracted by the method keeps an integral structure and is not degradable; and the acid-soluble collagen product has a denaturation temperature similar to that of collagen from a large terrestrial animal, can be used as a substitute for the collagen from the large terrestrial animal, and has a potential application value.

Description

【Technical field】 [0001] The invention belongs to the technical field of food. More specifically, the present invention relates to a method for extracting acid-soluble collagen from fish skin, and also relates to an acid-soluble collagen product obtained by the method. 【Background technique】 [0002] Collagen is a structural protein with a triple helix structure, and is widely used in food, medicine, biomedicine and cosmetic industries. Traditionally, the skin and bone of cattle and pigs are the main source of collagen, but with the outbreak of mad cow disease (Bovine spongiform encephalopathy, BSE) and foot-and-mouth disease (Foot-and-mouth disease, FMD) Concerns have arisen about the safety of animal-derived collagen and its related products. Also, collagen obtained from pigs and non-religious slaughter of cattle is traditionally unacceptable in Islam. Because of this safety and religious considerations, people began to turn their attention to aquatic animals, extractin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/78C07K1/14
Inventor 周鹏李天成区杨梁丽刘小鸣
Owner JIANGNAN UNIV
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