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6-kinetin molecularly imprinted polymer and application thereof

A technology of furfurylaminoadenine and molecular imprinting, which is applied in the field of analytical chemistry, can solve the problems of limited development, and achieve the effects of wide application range, increased sensitivity, and increased enrichment rate

Inactive Publication Date: 2013-12-11
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are still not many studies on pretreatment techniques such as MIP and SPME for the purification of CTKs

Method used

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  • 6-kinetin molecularly imprinted polymer and application thereof
  • 6-kinetin molecularly imprinted polymer and application thereof
  • 6-kinetin molecularly imprinted polymer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Preparation of MIP: A. Dissolve 17 mg template molecule KT and 150 μL methacrylic acid (functional monomer) in 3.6 mL absolute ethanol (porogen), shake well, place at room temperature for 12 h, then add 450 μL of trimethylolpropane trimethacrylate (cross-linking agent) and 34 mg of azobisisobutyronitrile (initiator) were used to obtain a pre-polymerization solution. After ultrasonication for 5 min, nitrogen gas was passed into the polymerization solution for 10 min. B. Add the above-mentioned pre-polymerization solution into a 2.0 mL derivatization vial, and place it in an oven at 60 °C for more than 8 hours. After the polymerization reaction is completed, KT-MIP will be generated.

Embodiment 2

[0048] Preparation of MISPE column: Prepare KT-MIP according to Example 1. In order to remove the template molecule KT, solid MIP is ground into powder, wrapped with filter paper, placed in a Soxhlet extractor, and 150 mL of absolute ethanol-acetic acid (90: 10, v / v) eluate, reflux at 85°C to elute the template molecule until KT is no longer detected by HPLC. Select several 5 mL syringe barrels, fill the polytetrafluoroethylene sieve plate in the bottom of the syringe barrel, weigh 250 mg of the treated KT-MIP and NIP powders into the above syringe barrels, beat the filler evenly, and then polymerize Another piece of polytetrafluoroethylene sieve plate was plugged on top of the object and compacted. Finally, MISPE column and NISPE column are produced.

Embodiment 3

[0050] Preparation of MISPME fiber coating: Take a 20 cm long quartz fiber, soak one end in acetone for 5 min, peel off the outer polyimide coating, and use secondary water, 1.0 mol / L NaOH, secondary water, Soak in 1 mol / L HCl, wash, dry, put the quartz fiber into 10% (v / v) γ-MAPS acetone solution for silanization treatment for 2 h, take it out, rinse it with methanol, and dry it with nitrogen. Prepare the pre-polymerization solution according to step A) of Example 1 and transfer it to a 2.0 mL derivatization vial, insert the silanized fiber, and react in an oven at 60 °C for 8 h. According to the same procedure and polymerization method, the same fiber is repeatedly coated with copolymerization to obtain a certain thickness of MISPME coating. After the coating was prepared, the template molecules were eluted with absolute ethanol-acetic acid (90:10, v / v) to remove template molecules until no template molecules were detected in the eluent.

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Abstract

The invention belongs to the field of the analytical chemistry, and particularly relates to a 6-kinetin molecularly imprinted polymer and application thereof to a filling material for a solid-phase extraction column or a fiber coating for solid-phase microextraction. According to the invention, a novel molecularly imprinted polymer (MIP) is synthesized by using 6-kinetin (KT) as a template molecule, methacrylic acid as a functional monomer, absolute ethyl alcohol as a pore-forming agent, trimethylolpropane trimethacrylate as a cross-linking agent and azodiisobutyronitrile as an initiator and by adopting a mass polymerization method. The KT-MIP is simple to prepare, is stable in property and strong in molecular recognition capability and can be used repeatedly. The prepared MIP is used as the coating for the SPME (solid-phase micro-extraction) and the filling material for the SPE (solid-phase extraction) and can selectively extract and enrich two targets with similar structures. By combining with a liquid-phase chromatographic detecting platform, the effective separation and the high-sensitivity detection of cytokinin can be realized, and the 6-kinetin molecularly imprinted polymer is used for the analysis of substrates such as a plant sample and an environmental sample.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and more specifically relates to a 6-furfurylaminoadenine molecularly imprinted polymer and its application in solid phase extraction column packing or solid phase microextraction fiber coating. Background technique [0002] Usually, a class of samples must undergo tedious sample pretreatment before analysis, the purpose of which is to separate the components to be measured from the complex sample matrix. Therefore, choosing a simple, efficient, and enrichment-capable pretreatment method often becomes a key factor. Among various pretreatment methods, liquid-liquid extraction (LLE) was developed the earliest, but it was gradually replaced by solid-phase extraction (SPE) due to the consumption of a large amount of organic solvent, easy emulsification, and time-consuming. In fact, SPE is a separation process of column chromatography. The analyte in the liquid sample is separated from the interfe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F120/06C08J9/26B01J20/26B01J20/285B01J20/30C03C25/28
Inventor 张兰卢巧梅范良彪陈国南
Owner FUZHOU UNIV
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