Hybridization breeding method for hypsizygus marmoreus

Abstract

The invention discloses a hybridization breeding method for hypsizygus marmoreus. The method includes the steps of subjecting RF24 and RF30 to hypha activation, performing optimized cultivation on superior panels respectively, then subjecting the RF24 and the RF30 to strain purification on superior panels respectively, selecting exuberant at the top and purified two varieties for hypha hybridization after cultivation, performing screening cultivation when hyphae of two inoculation positions are combined and overgrow the panels after the hypha hybridization, selecting the panels which have the same hypha growing rate and combine together for expanding propagation cultivation after the screening cultivation, performing cultivation on the selected panels for 14 days after the expanding propagation, transferring to a conical flask for 37 days of cultivation and then transferring to a stock seed cultivation bottle for 45 days of growth, transferring to a cultivation bottle for 85 days and performing mycelium stimulation for fruiting, selecting the original strain with the highest yield for tissue isolation and mycelium cultivation, and preserving the strain to a test tube. The hybridization breeding method for the hypsizygus marmoreus can improve quality and yield of the hypsizygus marmoreus, greatly improve breeding efficiency, and is suitable for industrial cultivation of factory production.

Description

technical field [0001] The invention relates to the technical field of edible fungus cultivation, in particular to a method for hybridization and breeding of mushrooms. Background technique [0002] True jiji mushroom, also known as jade mushroom, spot jade mushroom, lotus leaf lily mushroom, belongs to Basidiomycotina, layer fungi, agaricales, white mushroom family, and jade mushroom genus. It has the characteristics of beautiful appearance, unique flavor, and rich nutrition. It is a rare edible fungus that has become popular in the Japanese market in recent years and is favored by consumers. The shape of the mushroom is beautiful, the texture is crisp and tender, and the taste is delicious and unique. In Japan, people often compare it with the precious matsutake, and it is dubbed "false matsutake" and "crab-flavored mushroom", and enjoys the reputation of "matsutake when you hear it, and jade when you eat it". [0003] my country is in March, 1986, by the Dalian Branch o...

AI Technical Summary

Problems solved by technology

In the first few years of the introduction of Jadegrass strains to my country, due to the confusion of the Chinese translation of the name, foreign businessmen introduced the information of Shiji mushrooms very easily, and it was difficult for trial planters to find detailed information on the biological characteristics of Shiji mushrooms. The technology and equipment used are difficult to promote and apply in my country