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Method for quickly identifying johnsongrass and kindred plant seeds

A technology for plant seeds and pseudo-sorghum, which is applied to the field of rapid identification of quarantine weed pseudo-sorghum seeds and seeds of related species, can solve the problems of complicated operation, difficult shape, long time and special equipment, and achieves a simple identification method, Reliable results, avoiding losses

Inactive Publication Date: 2012-08-29
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the seeds of sorghum plants are similar, it is difficult to effectively distinguish them in shape, and the effectiveness of this method is based on long-term work experience; false sorghum and black sorghum have the same number of chromosomes, very similar karyotypes, and more importantly, karyotypes. Type analysis requires root tip shoots, complicated operation, and the reagents and drugs used are highly toxic, and it takes up to several weeks to actually implement it, which is difficult to meet the requirements of fast customs clearance of goods; biochemical and molecular biology methods involve many drugs. , the operation is complicated, and the implementation of biochemical methods such as molecular markers takes a long time and special equipment, and the cost is also high

Method used

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  • Method for quickly identifying johnsongrass and kindred plant seeds
  • Method for quickly identifying johnsongrass and kindred plant seeds
  • Method for quickly identifying johnsongrass and kindred plant seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 The difference between false sorghum and black sorghum

[0026] Weigh 3 parts of false sorghum seeds and 3 parts of the target species to be identified (black sorghum here), 0.2g each, add 30ml of analytically pure petroleum ether, soak at 25°C for 2.5h, filter, and dilute to 100ml. Put the above petroleum ether extract into a 1cm quartz cuvette, use the corresponding solvent as a blank control, and scan on an ultraviolet spectrophotometer (unicam uv540 ultraviolet spectrophotometer). Wavelength range: 200-400nm; slit: 1nm; scanning speed: 0.5s / SCAN, acquisition 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297 ,298,299,300,301,302,303,304,305,306,307,308,309,310,311,312,313,314,315,316,317,318,319,320,321,322 , 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 342, 349, 354, 362, 366, 369, 379, 384, 385, 387, 390, 399, 400nm absorbance data ( See Schedule). Based on 6 sets of UV absorbance data of 63 sensitive bands, the cluster ...

Embodiment 2

[0027] Embodiment 2 The difference between false sorghum and sorghum

[0028] Weigh 3 parts of false sorghum seeds and 3 parts of the target species to be identified (sorghum here), 0.2g each, add 30ml of analytically pure petroleum ether, soak at 25°C for 2.5h, filter, and dilute to 100ml. Put the above petroleum ether extract into a 1cm quartz cuvette, use the corresponding solvent as a blank control, and scan on an ultraviolet spectrophotometer (unicam uv540 type ultraviolet spectrophotometer). Wavelength range: 200-400nm; slit: 1nm; scanning speed: 0.5s / SCAN, acquisition 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297 ,298,299,300,301,302,303,304,305,306,307,308,309,310,311,312,313,314,315,316,317,318,319,320,321,322 , 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 342, 349, 354, 362, 366, 369, 379, 384, 385, 387, 390, 399, 400nm absorbance data ( See Schedule). Based on 6 sets of UV absorbance data of 63 sensitive bands, the cluster analysi...

Embodiment 3

[0029] Embodiment 3 The difference between false sorghum and Sudan grass

[0030] Weigh 3 parts of false sorghum seeds and 3 parts of the target species to be identified (Sudan grass here), 0.2g each, add 30ml of pure petroleum ether, soak at 25°C for 2.5h, filter, and dilute to 100ml. Put the above petroleum ether extract into a 1cm quartz cuvette, use the corresponding solvent as a blank control, and scan on an ultraviolet spectrophotometer (unicam uv540 ultraviolet spectrophotometer). Wavelength range: 200-400nm; slit: 1nm; scanning speed: 0.5s / SCAN, acquisition 283, 284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297 ,298,299,300,301,302,303,304,305,306,307,308,309,310,311,312,313,314,315,316,317,318,319,320,321,322 , 323, 324, 325, 326, 327, 328, 329, 330, 331, 332, 342, 349, 354, 362, 366, 369, 379, 384, 385, 387, 390, 399, 400nm absorbance data ( See Schedule). Based on 6 sets of UV absorbance data in 63 sensitive bands, using the cluster analysis mo...

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PUM

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Abstract

The invention provides a technical method for quickly and rightly identifying johnsongrass and kindred plant seeds. Seed impregnation liquid of johnsongrass and kindred plant seeds is obtained through solvent extraction and impregnation, then an ultraviolet absorbency value on a specific sensitive wave band is detected, cluster analysis charts of the johnsongrass and target species requiring identification are generated by using computer software, further the cluster analysis charts are analyzed and identified, so that the johnsongrass and the kindred plants are distinguished and the target species are identified. The method for identifying is simple, the results are reliable, the cost is low, the loss caused by error identification can be avoided, and the method is significant in plant quarantine inspection.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a technical method for quickly identifying the seeds of the quarantine weed Pseudosorghum and the seeds of the same genus. Background technique [0002] Sorghum halepense (L.) Pers is a perennial weed of the genus Sorghum in the grass family. Sorghum halepense (L.) Pers is native to the Mediterranean region and is one of the ten most harmful and difficult to control weeds in the world. [0003] False sorghum has strong tillering ability, well-developed rhizomes, and the underground rhizomes have a wide range of expansion, which is difficult to pull out. False sorghum mainly reproduces with underground stems and seeds, and its underground rhizomes have strong reproductive power. Still sprouting new shoots. A false sorghum plant can produce 28,000 seeds, and the seeds are easy to fall off, often mixed with sowing materials or commercial grains, especially easy to spread with the lefto...

Claims

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Application Information

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IPC IPC(8): G01N21/33
Inventor 郭水良娄玉霞印丽萍
Owner SHANGHAI NORMAL UNIVERSITY
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