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Method for detecting bar-transgenic sugarcane and appliance

A technology of sugarcane and genes, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of cumbersome operation, complicated procedure, high cost, etc., and achieve the effect of uniform treatment, simple operation and accurate effect

Inactive Publication Date: 2010-04-28
深圳市千年盛世基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods all require certain experimental conditions, complex procedures, cumbersome operations, and high costs.
In particular, the first two methods cannot determine the expression level of foreign genes
The smearing method is susceptible to external influences and is inconsistent, which brings errors to the experiment and leads to inaccurate results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The test materials used were sugarcane variety Xintaitang No. 20 and the transgenic bar gene line using it as the recipient, and the following test steps were included in sequence:

[0019] 1. Sensitivity test of wild-type sugarcane leaves:

[0020] (1) prepare PPT solutions of different concentrations, the concentrations are respectively: prepare the PPT solutions of different concentration gradients with deionized water, the concentrations are respectively: 0, 50, 100, 150, 200, 250 and 300ppm (mass volume ratio, 100ppm is 1g PPT is dissolved in 10L deionized water, and the other is the same);

[0021] (2) Get 7 clean 10ml glass test tubes, add 5ml PPT solution to each test tube respectively, obtain 7 test tubes containing different PPT concentrations;

[0022] (3) Select wild-type sugarcane leaves with consistent growth parts, cut into small sections of about 5 cm, and put them into 7 glass test tubes containing different PPT concentrations;

[0023] (4) Put the te...

Embodiment 2

[0033] The test materials used were sugarcane variety Xintaitang No. 20 and the transgenic bar gene line using it as the recipient, and the following test steps were included in sequence:

[0034] 1. Sensitivity test of wild-type sugarcane leaves:

[0035] (1) Prepare PPT solutions of different concentrations, the concentrations are respectively: prepare PPT solutions with different concentration gradients with deionized water, the concentrations are respectively: 0, 50, 100, 150, 200, 250 and 300ppm (mass volume ratio);

[0036] (2) Get 7 clean 10ml glass test tubes, add 5ml PPT solution to each test tube respectively, obtain 7 test tubes containing different PPT concentrations;

[0037] (3) Select wild-type sugarcane leaves with consistent growth parts, cut into small sections of about 2 cm, and put them into 7 glass test tubes containing different PPT concentrations;

[0038] (4) Put the test tube in an artificial climate box, culture conditions: temperature 25°C, light 20...

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Abstract

The invention discloses a method for detecting bar-transgenic sugarcane and appliance. The method comprises the steps of: directly immersing sugarcane leaves to be detected into herbicide solution and processing; and judging the result by directly observing the damage level, compared with the bar gene-free sugarcane leaves. The method is used for appraising bar gene-containing sugarcane plant, is especially suitable for the fast diction of the transgenosis regeneration plant, and can greatly reduce the detection for the molecule of the transgenosis plant, greatly improve the detection efficiency, and reduce the detection cost.

Description

technical field [0001] The invention relates to a method for detecting transgenic herbicide-resistant sugarcane, in particular to a method and application for detecting transgenic bar gene sugarcane. Background technique [0002] With the development of molecular biology, the application of transgenic technology in crop breeding is becoming more and more extensive. At present, the area of ​​genetically modified crops is increasing year by year, and herbicide-resistant genetically modified crops account for the largest proportion of the currently planted genetically modified crops. Weeds bring huge losses to sugarcane production every year, and the development of herbicide-resistant transgenic sugarcane has great economic value. The bar gene is a widely used herbicide-resistant gene. The introduction of this gene can improve the tolerance of sugarcane to the herbicide BASTA, so that the weeds can be effectively killed by spraying the herbicide in production, and the herbicid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 王继华曹干张剑亮
Owner 深圳市千年盛世基因科技有限公司
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