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Method for extracting heparin sodium from cattle lungs

A technology of heparin sodium and bovine lung, which is applied in the field of extracting heparin sodium, can solve the problems of low product yield, low product yield, and high production cost, and achieve the effects of simplifying the production process, reducing production cost, and improving yield and quality

Inactive Publication Date: 2012-08-15
BAIYIN FURUIKANG BIOLOGICAL PRODS
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Problems solved by technology

At present, there is no large-scale production in China. The main reason is that the filtration is difficult and the product yield is low. The traditional method of producing heparin sodium from pig small intestine is followed, that is, resin adsorption, washing, elution and resin regeneration, causing environmental pollution. The production cost is high, the product yield is low, and the titer is low. This method is difficult to scale up

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  • Method for extracting heparin sodium from cattle lungs
  • Method for extracting heparin sodium from cattle lungs

Examples

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Embodiment 1

[0019] Example 1: See figure 1 , A method for extracting heparin sodium from bovine lungs, characterized in that it comprises the following steps:

[0020] (1) Pulping: remove the large trachea and external cortical fat in the bovine lung, mince it, and homogenize it into a slurry, bovine lung: water = 1:2-3, adjust the pH to 9;

[0021] (2) Enzymatic hydrolysis: heat the above slurry to 39-41°C, add protease according to 1.5-3.5% of the slurry mass, carry out enzymatic hydrolysis for 3-4 hours, monitor the change of pH, control the pH to 7.5-8.5, filter enzymatic hydrolysis Then add heparin sodium special complex enzyme at 0.5-1.5% of the mass of the slurry, raise the temperature to 49-51 degrees, control the pH to 7.5-8.5, keep the temperature for 3-3.5 hours, and monitor the titer of the enzyme hydrolysate. End of change enzymatic hydrolysis;

[0022] (3) Enzyme inactivation: add protein precipitation agent and NaCL to the enzymatic hydrolysate obtained in step (2). The protein ...

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Abstract

The invention relates to a method for extracting heparin sodium from cattle lungs. The method comprises the following steps of: (1), slurring: (2), enzymolysis; (3), enzyme removing; (4) purification; and (5), freeze drying. The method can be used for overcoming the difficulties in filtration of an enzymolysis liquid in the production of the heparin sodium from the cattle lungs, avoiding the trivial procedures of long-time resin adsorption, washing elution, resin regeneration and the like, solving the problems of environment pollution caused by acid-base waste liquor and the like, simplifying the production process, reducing the production cost, improving the yield and quality of products, reducing the environment pollution and providing an effective production guarantee for the scale production of the lung heparin sodium. The yield of the products can reach 42,000u / kg (of the lungs), and the titer reaches 70-120u / mg.

Description

Technical field [0001] The invention belongs to the field of biological product processing, and particularly relates to a method for extracting heparin sodium from bovine lungs Background technique [0002] Heparin sodium is a kind of mucopolysaccharide contained in mammals. Heparin sodium is combined with protein and exists in the intestinal mucosa, lung, liver and other organs. After heparin sodium and protein are separated and extracted, it has anticoagulant, antithrombotic, and hypolipidemic properties. Anti-inflammatory and many other physiological properties, it is a markedly effective drug for preventing and treating atherosclerosis and cardiovascular and cerebrovascular diseases. At present, there is no large-scale production in China, mainly due to the difficulty of filtration and low product yield. The traditional methods of producing heparin sodium from the pig small intestine are used, namely resin adsorption, washing, elution and resin regeneration treatment, which c...

Claims

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Application Information

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IPC IPC(8): C08B37/10
Inventor 徐殿英李作红陈勇司建奎
Owner BAIYIN FURUIKANG BIOLOGICAL PRODS
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