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Method for preparing chitosan through chitin liquefaction

A technology for liquefying chitosan and oxidizing chitosan, which is applied in the production of bulk chemicals and can solve problems such as environmental pollution of chitosan

Pending Publication Date: 2021-09-10
荣成万盈水产科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the severe environmental pollution of the existing alkaline method for extracting chitosan, the purpose of the present invention is to provide a method for preparing chitosan by chitin liquefaction with simple and efficient process flow, green and pollution-free

Method used

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  • Method for preparing chitosan through chitin liquefaction
  • Method for preparing chitosan through chitin liquefaction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A kind of chitin liquefaction prepares the method for chitosan, it passes through following processing steps:

[0026] 1) Degradation of chitin

[0027] a. Take a certain amount of squid fan-shaped bone and pulverize it with an ultrafine pulverizer to obtain squid fan-shaped bone powder;

[0028] b. Add hydrogen peroxide to the squid fan-shaped bone powder obtained in step a, rapidly heat up to 120°C hydrogen peroxide detonation point, and process to obtain oxidized chitosan; wherein the hydrogen peroxide has a concentration of 67%, soak the squid fan-shaped bone powder in hydrogen peroxide before heating 2.5 hours;

[0029] 2) Enzymolysis

[0030] Adding the oxidized chitosan obtained in step b to a composite protease, enzymatically hydrolyzing it for 10 h under the action of ultrasonic waves, centrifuging and taking the clear liquid to obtain crude liquefied chitosan; wherein, the composite protease includes alkaline protease and deacetylase, The mass percentage of...

Embodiment 2

[0034] A kind of chitin liquefaction prepares the method for chitosan, it passes through following processing steps:

[0035] 1) Degradation of chitin

[0036] a. Take a certain amount of squid fan-shaped bone and pulverize it with an ultrafine pulverizer to obtain squid fan-shaped bone powder;

[0037] b. add the squid fan-shaped bone powder obtained in step a to hydrogen peroxide, rapidly heat up to the detonation point of hydrogen peroxide at 100°C, and the treatment is completed to obtain oxidized chitosan; wherein, the hydrogen peroxide has a concentration of 60%, soak squid in hydrogen peroxide before heating Fan-shaped bone meal 2h;

[0038] 2) Enzymolysis

[0039] Adding the oxidized chitosan obtained in step b to a composite protease, enzymatically hydrolyzing it for 10 h under the action of ultrasonic waves, centrifuging and taking the clear liquid to obtain crude liquefied chitosan; wherein, the composite protease includes alkaline protease and deacetylase, The m...

Embodiment 3

[0043] A kind of chitin liquefaction prepares the method for chitosan, it passes through following processing steps:

[0044] 1) Degradation of chitin

[0045] a. Take a certain amount of squid fan-shaped bone and pulverize it with an ultrafine pulverizer to obtain squid fan-shaped bone powder;

[0046] b. Add hydrogen peroxide to the squid fan-shaped bone powder obtained in step a, and rapidly heat up to 150°C hydrogen peroxide detonation point, and the treatment is completed to obtain oxidized chitosan; wherein, the hydrogen peroxide has a concentration of 70%, soak squid fan-shaped in hydrogen peroxide before heating Bone meal 3h;

[0047] 2) Enzymolysis

[0048] Add the oxidized chitosan composite protease obtained in step b, enzymolyze it for 12 hours under the action of ultrasonic waves, centrifuge and take the clear liquid to obtain crude liquefied chitosan; wherein, the composite protease includes alkaline protease and deacetylase, Its alkaline protease and deacetyl...

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Abstract

The invention discloses a method for preparing chitosan through chitin liquefaction. The method solves the problem that the environment is polluted by waste liquid and waste residues generated by high-concentration acid and alkali treatment needed in existing chitin preparation. The method comprises the following steps: 1) degrading chitin; 2) performing enzymolysis; and 3) purifying. The method comprises the steps: by taking fan-shaped squid bones as raw materials, soaking the fan-shaped squid bones in hydrogen peroxide, performing oxidative degradation on the fan-shaped squid bones by utilizing the explosion concentration of the hydrogen peroxide and using a controllable explosion technology to obtain degraded chitin, adding compound enzyme, further decomposing the oxidized and degraded chitin, and removing impurities through carbon dioxide supercritical fluid to obtain the chitosan. The chitosan extraction rate of the process is 42%. The hydrogen peroxide blasting combined enzyme method is adopted, the prepared chitosan is large in molecular weight, the product is good in biocompatibility and water solubility, and the technological process is simple, efficient, green and free of pollution.

Description

technical field [0001] The invention relates to the preparation of chitin polysaccharide, in particular to a method for preparing chitosan by liquefying chitin. Background technique [0002] As we all know, squid fan-shaped bones, shrimp heads and shrimp shells and other aquatic leftovers are rich in nutrients and rich in chitin, and the chitin content is 3-5%. Except for recovered protein, most of them are directly dried and used as feed, or even discarded as garbage. [0003] At present, industrial chitosan extraction is mainly made from the shells of animals such as shrimp and crabs. The preparation process of chitosan is mainly acid-base method, using hydrochloric acid to decalcify, sodium hydroxide to deproteinize, and low-concentration lye to deproteinize. In addition to acetyl. Although the extraction of chitosan by this acid-base method is relatively low in cost and simple in operation, it requires a large amount of high-concentration acid and alkali, which not onl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
CPCC08B37/003C08B37/0003Y02P20/54
Inventor 杨劲峰吴文惠毕进红
Owner 荣成万盈水产科技有限公司
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