Extraction process of alkaloid in thunberg fritillary bulb

An extraction process, the technology of Fritillaria japonicus, is applied in the field of extraction of alkaloids from Fritillaria japonicus, which can solve the problems of unfavorable industrial production, poor extraction rate of alkaloids, and aggravated environmental pollution, so as to shorten drying time and reduce impurities in sediments. Less, to ensure full use of the effect

Active Publication Date: 2012-08-15
TIANJIN PHARMA GROUP XINZHENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, the traditional alkaloid extraction method has cumbersome steps, poor alkaloid extraction rate, and low product yield. It uses flammable, explosive, and toxic organic solvents, which increases the cost and aggravates the impact on the environment. Pollution, at the same time, due to the long extraction cycle, it is not conducive to industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Take 60kg of Fritillaria japonicus and crush it below 4mm, soak it in a mixture containing hydrochloric acid and 70% ethanol solution, the volume concentration of hydrochloric acid in the mixture is 0.1%, the temperature is 50°C, and the time is 12 hours; Mother carries out reflux extraction, and extraction temperature is 90 ℃, and the amount of mixed solution during extraction is 360kg, and extraction time is 2 hours, and filtered gained extract is standby, and 360kg mixed solution is added in remaining medical material and carries out next reflux extraction, and reflux extraction is altogether 3 time; every reflux extraction gained extract is combined and concentrated under reduced pressure with a spherical concentrator, and the vacuum degree is 0.08MPa during concentration, and the concentrated extract is to 12kg, and then 50% sodium hydroxide solution is added to adjust the pH value of the concentrate to 4.4. Filtration. The precipitate was dried in a microwave vacuu...

Embodiment 2

[0013] Take 60kg of Fritillaria japonicus and crush it to less than 4mm, soak it in a mixture containing hydrochloric acid and 75% ethanol solution, the volume concentration of hydrochloric acid in the mixture is 0.2%, the temperature is 55°C, and the time is 14 hours; The mother carries out reflux extraction, the extraction temperature is 85 ℃, the amount of mixed solution during extraction is 300kg, the extraction time is 2.5 hours, the obtained extract is filtered for use, and 300kg of mixed solution is added to the remaining medicinal materials for the next reflux extraction; two reflux extractions Gained extracts are combined and concentrated under reduced pressure with a spherical concentrator. When concentrating, the vacuum is 0.08MPa, and the extract is concentrated to 15kg. Then, 50% sodium hydroxide solution is added to adjust the pH value of the concentrate to 4.6, and the precipitate is filtered. After being dried in a microwave vacuum dryer at 70°C, it was crushed ...

Embodiment 3

[0015] Take 60kg of Fritillaria japonicus and crush it below 4mm, soak it in a mixture containing hydrochloric acid and 80% ethanol solution, the volume concentration of hydrochloric acid in the mixture is 0.5%, the temperature is 70°C, and the time is 10 hours; Mother carries out reflux extraction, and extraction temperature is 98 ℃, and the amount of mixed solution during extraction is 600kg, and extraction time is 4 hours, filters; After filtering the obtained extraction solution, carry out decompression concentration with spherical concentrator, and vacuum degree is 0.07MPa during concentration, Concentrate the extract to 20kg, then add 50% sodium hydroxide solution to adjust the pH value of the concentrate to 5, filter, and dry the precipitate at 70°C in a microwave vacuum dryer, then crush it through a 80-mesh sieve, and continue to concentrate the filtrate until the relative density is 1.36 thick paste. After testing, the total amount of alkaloids (peiminin A and peimin...

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Abstract

The invention belongs to the technical field of effective component extraction of Chinese medicaments, and in particular relates to an extraction process of alkaloid in thunberg fritillary bulb. The process comprises the following steps: crushing the thunberg fritillary bulb; warmly soaking in mixed solution of hydrochloric acid and ethanol, and refluxing to extract; concentrating the extract in reduced pressure, and filtering; and drying precipitate. According to the extraction process, hydrochloric acid is directly added into an ethanol solution for soaking and extracting, the extraction efficiency can be improved, leaching of alkaloids can be promoted, the stability of the preparation is improved, acid insoluble impurities can be removed or reduced, the production efficiency can be improved, steps after ethanol extraction, concentration and acid water solution can be saved, and the production operation is simplified.

Description

technical field [0001] The invention belongs to the technical field of extraction of effective components of traditional Chinese medicines, and in particular relates to an extraction process of alkaloids in Fritillaria japonicus. Background technique [0002] Fritillaria fritillaria is bitter in taste, cold in nature, has the effect of clearing away heat and resolving phlegm, resolving stagnation and resolving stagnation, and is used for wind-heat, dryness-heat, phlegm-heat cough, lung abscess, mastitis, scrofula, sores, etc. Fritillary Fritillaria mainly contains alkaloids: Fritillary Fritillary Alkaloid, Fritillary Fritillin B, Peiminin, Isopeiminine, Fritillin, Fritidine, Proto-Peiminine, Zhebeining, Fritillaria Two isomers of Mother B; Others also contain calcinol, phytosterol, carotene and so on. Modern pharmacology and clinical research results show that the alkaloids in Fritillaria have antitussive, analgesic and sedative effects. [0003] At present, the extraction...

Claims

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Application Information

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IPC IPC(8): A61K36/8966A61P11/14A61P29/00A61P25/20
Inventor 刘博李俊霞程永科白艳鹤
Owner TIANJIN PHARMA GROUP XINZHENG
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