Breeding method for three-line ultra-small-embryo hybrid rice
A hybrid rice and seed technology is applied in the field of selection and breeding of three-line extra-small embryo hybrid rice, which can solve the problem of low yield and achieve the effects of increasing thousand-grain weight, high breeding efficiency and increasing farmers' income.
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Embodiment 1
[0015] Take 700 grams each of rice three-line restorer line FR316 and maintainer line T55B seeds, soak the seeds for 24 hours, accelerate germination at 30°C for 18-24 hours until the seed buds are white, and treat them with a mixture of chemical mutagen manganese sulfate and diethyl sulfate (DES) 6 hours; after treatment, the seeds were rinsed twice with water; 0 ), Mixed M 0 Plant outgrown plants (M 1 ) on the seed (M 2 ); M 2 After drying, the husks were removed to form brown rice, and the extra-small-embryo mutant brown rice kernels were visually selected from the brown rice; the extra-small-embryo mutant brown rice kernels of FR316 and T55B were planted respectively, and FR316 and T55B were used as controls, and the agronomic traits of all mutants were evaluated at the heading and flowering stage. The FR316 ultra-small embryo mutant with similar agronomic traits to FR316 and good flowering and loose powder traits was selected as the three-line ultra-small embryo resto...
Embodiment 2
[0017] Take 700 grams of the seeds of the three-line restorer line Shuhui 527 and the maintainer line D62B each, soak the seeds for 24 hours, accelerate germination at 30°C for 18-24 hours until the seed buds are white, and mix with chemical mutagen manganese sulfate and diethyl sulfate (DES) solution treatment for 4 hours; after the treatment, the seeds were rinsed twice with water; 0 ), Mixed M 0 Plant outgrown plants (M 1 ) on the seed (M 2 ); M 2 After drying, the husks were removed to form brown rice, and the extra-small embryo mutant brown rice grains were visually selected from the brown rice; the extra-small-embryo mutant brown rice grains of Shuhui 527 and D62B were planted respectively, and Shuhui 527 and D62B were used as controls, and all mutant brown rice grains were selected at the heading and flowering stage. The agronomic traits and flowering and loose powder traits of the mutants were investigated, and the super small embryo mutant of Shuhui 527 with simil...
Embodiment 3
[0019] Take 700 grams each of the rice three-line restorer line Minghui 86 and maintainer line II-32B seeds, soak the seeds for 24 hours, accelerate germination at 30°C for 18-24 hours until the seed buds are white, and use chemical mutagen manganese sulfate and diethyl sulfate (DES). ) mixed solution for 5 hours; after the treatment, the seeds were rinsed twice with water; 0 ), Mixed M 0 Plant outgrown plants (M 1 ) on the seed (M 2 ); M 2 After drying, the husks are removed to form brown rice, and the extra-small embryo mutant brown rice grains are visually selected from the brown rice; Minghui 86 and II-32B extra-small-embryo mutant brown rice kernels are planted respectively, and Minghui 86 and II-32B are used as controls, and flowering occurs at heading The agronomic traits and flowering and pollen-losing traits of all mutants were investigated in the early stage, and the super-small embryo mutant of Minghui 86 with similar agronomic traits to Minghui 86 and good flow...
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