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Preparation method of fiber carrier-based western blotting calcium alginate hybrid gel membrane

A Western blotting and alginic acid technology, applied in dry cleaning fiber products, fiber types, fiber processing and other directions, can solve the problems of low calcium alginate hydrogel strength, protein denaturation, low imprinting efficiency, etc., and achieve excellent biocompatibility , the effect of simple operation

Inactive Publication Date: 2012-08-01
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies of the prior art, the technical problem to be solved by the present invention is that the protein will be denatured when encountering organic matter, the strength of the pure calcium alginate hydrogel is low, and the imprinting efficiency is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1. Phosphoric acid / calcium alginate hybrid gel membrane imprinted with bovine serum albumin based on polypropylene fiber non-woven fabric carrier

[0017] Put the polypropylene fiber non-woven fabric into the acrylic acid aqueous solution with a volume percentage of 1%, add a polymerization inhibitor ferrous ammonium sulfate with a mass percentage concentration of 0.5%, pass through nitrogen for protection, and irradiate with a 500W ultraviolet lamp at room temperature After 1 hour, take it out and rinse it with water for 3 to 5 times to remove unreacted monomers and homopolymers to obtain a polypropylene fiber non-woven fabric grafted with acrylic acid, and then neutralize it with an aqueous sodium hydroxide solution with a concentration of 1% by mass. , to obtain the polypropylene fiber non-woven fabric grafted by sodium polyacrylate;

[0018] Add sodium alginate and diammonium hydrogen phosphate to the bovine serum albumin solution with a mass percentage conc...

Embodiment 2

[0021] Example 2. Lysozyme imprinted phosphoric acid / calcium alginate hybrid gel membrane based on polypropylene fiber non-woven carrier

[0022] Put the polypropylene fiber non-woven fabric into the acrylic acid aqueous solution with a volume percentage of 5%, add a polymerization inhibitor ferrous ammonium sulfate with a mass percentage concentration of 0.5%, pass through nitrogen for protection, and irradiate with a 500W ultraviolet lamp at room temperature After 1 hour, take it out and rinse it with water for 3 to 5 times to remove unreacted monomers and homopolymers to obtain a polypropylene fiber non-woven fabric grafted with acrylic acid, and then neutralize it with a 5% sodium hydroxide aqueous solution , to obtain a fiber non-woven fabric grafted with sodium polyacrylate;

[0023] Add sodium alginate and diammonium hydrogen phosphate to the lysozyme solution with a concentration of 0.1% by mass, stir to make it fully dissolve, and obtain a mixed solution of sodium alg...

Embodiment 3

[0026] Example 3. Bovine hemoglobin imprinted polyacrylic acid / calcium alginate hybrid gel membrane based on polypropylene fiber monofilament carrier

[0027] Put the polypropylene fiber monofilament into the acrylic acid aqueous solution of 50% by volume, add the polymerization inhibitor ferrous ammonium sulfate with a concentration of 3% by mass, feed nitrogen as protection, and irradiate 0.5% with a 500W ultraviolet lamp at room temperature. hours, take it out and rinse it with water for 3 to 5 times to remove unreacted monomers and homopolymers to obtain polyene fiber monofilaments grafted with acrylic acid, and then neutralize it with a 10% sodium hydroxide aqueous solution, Obtain the fiber monofilament of sodium polyacrylate graft;

[0028]Add sodium alginate and sodium polyacrylate to the bovine hemoglobin solution with a mass percentage concentration of 0.2%, stir to make it fully dissolve, and obtain a mixed solution with a mass percentage concentration of 2.5% sodiu...

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PUM

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Abstract

The invention provides a preparation method of a fiber carrier-based western blotting calcium alginate hybrid gel membrane. The preparation method comprises the following steps: putting fibers in an aqueous solution of acrylic acid, adding a polymerization inhibitor ammonium ferrous sulfate, letting nitrogen in, irradiating with an ultraviolet lamp for 0.2-1h to obtain polyacrylic acid grafted fibers, and neutralizing with an aqueous solution of sodium hydroxide to obtain sodium polyacrylate grafted fibers; preparing a mixed aqueous solution containing proteins with the mass percentage concentration of 0.1-5%, sodium alginate with the mass percentage concentration of 2-5% and a water-soluble additive with the mass percentage concentration of 0.05-5%, and simultaneously preparing an aqueous solution of calcium chloride with the mass percentage concentration of 1-10%; and immersing the sodium polyacrylate grafted fibers in the mixed aqueous solution of the proteins, the water-soluble additive and sodium alginate, then immediately putting the fibers in the aqueous solution of calcium chloride to make the fibers crosslink for 0.5-2h, and washing off the template proteins to obtain the fiber carrier-based western blotting calcium alginate hybrid gel membrane.

Description

technical field [0001] The invention relates to a preparation method of a protein imprinted calcium alginate hybrid gel film based on a fiber carrier, and belongs to the field of functional polymer materials. Background technique [0002] Molecular imprinting technology is a technology for preparing polymers with high affinity for specific target molecules. At present, great progress has been made in the research on the specific recognition of small molecules, such as amino acids, sugars and their derivatives. and drugs etc. However, the research on the imprinting of biological macromolecules, such as proteins, is far less systematic and in-depth than the research on small molecule imprinting. Protein molecularly imprinted polymers have become a current research hotspot due to their potential applications in medical diagnosis, bioseparation and drug delivery [Chemistry Progress. 2008, 20(8): 957-968]. Due to the large size of protein molecules, easy denaturation, and very ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D06M14/18D06M14/34D06M14/28D06M14/30D06M15/13D06M15/15D06M16/00D06M11/155C12S11/00D06L1/00D06M101/34D06M101/20D06M101/22D06M101/28D06M101/30D06L1/12
Inventor 赵孔银魏俊富李东英李会隋丽芳
Owner TIANJIN POLYTECHNIC UNIV
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