Application for isobenzofuran type compounds in marine biofouling prevention and preparation method thereof

A technology for marine biofouling and compounds, which is applied to the application of isobenzofuran compounds in preventing marine biofouling, and the field of preparation of isobenzofuran compounds, which can solve the problem of complex preparation processes of natural non-toxic compounds and marine biofouling. Ecological environment damage and other problems, to achieve the effect of strong inhibition of biofouling activity, high purity, and simple source of raw materials

Inactive Publication Date: 2014-12-03
YUNNAN MINZU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] In order to solve the problem in the background that traditional anti-marine biofouling and antifouling agents are easy to cause damage to the marine ecological environment, and the preparation process of natural non-toxic compounds with anti-marine biofouling activity is relatively complicated, the present invention aims to provide a Application of isobenzofuran compounds to inhibit marine biofouling and method for preparing isobenzofuran compounds

Method used

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  • Application for isobenzofuran type compounds in marine biofouling prevention and preparation method thereof
  • Application for isobenzofuran type compounds in marine biofouling prevention and preparation method thereof
  • Application for isobenzofuran type compounds in marine biofouling prevention and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0036] ——The inhibitory activity of cephalosporium AL031 fungal extract on the attachment of barnacle larvae.

[0037] Preparation of cephalosporium AL031 fungal solid fermentation product ethyl acetate extract: using rice, corn flour, bagasse, wheat bran as raw materials, mixing and sterilizing at a ratio of 10: 2: 3: 3 (W / W / W / W) ,cool down. Cephalosporium AL031 fungus was inoculated into the mixture, fermented at 28 ℃, fermented for 6 days, the strain preservation number is CCTCC: M2010140; cold leaching with industrial ethyl acetate in the dark for 3 days, extraction 3 times, the extract is filtered and reduced Press and concentrate to obtain ethyl acetate extract and store in the dark.

[0038] An experimental model for inhibiting the attachment of larvae of Barnacle Venus is used (see scientific literature: Xu Y., He HP, Qian P. Y, et al. Potent antifouling compounds produced by marine streptomyces. Bioresource Technology. 2010,101(4): 1331-1336) tested the ability of the et...

Embodiment 2

[0044] —— 4,5,6-trihydroxy-7-methyl-1,3-dihydroisobenzofuran and 4,5,6-trihydroxy-7-methyl-3H-isobenzofuran-1-one preparation.

[0045] The separation and identification process of anti-biofouling active ingredients in the solid fermentation product of Cephalosporium AL031 fungus is as follows figure 1 Shown.

[0046] Take rice, corn meal, bagasse, wheat bran as raw materials, mix and sterilize and cool at a ratio of 10: 2: 3: 3 (W / W / W / W); classify the strain as the fungal inoculation of Cephalosporium AL031 In the mixed medium, fermented at 28 ℃ for 6 days, the strain preservation number is CCTCC: M2010140; the solid fermentation product was extracted with heavy steamed industrial ethyl acetate in the dark and cold leached for 3 days, extracted 3 times, combined the extracts, Filter and concentrate the extract under reduced pressure to obtain ethyl acetate extract (40 g). Disperse the above-mentioned heavy distilled industrial ethyl acetate extract in water. Use 10 g of heavy di...

Embodiment 3

[0053] ——The compound 4,5,6-trihydroxy-7-methyl-1,3-dihydroisobenzofuran and 4,5,6-trihydroxy-7-methyl-3H-isobenzofuran-1 -Determination of the activity of ketones against the attachment of barnacle larvae.

[0054] An experimental model for inhibiting the attachment of larvae of Barnacle Venus is used (see the scientific literature: Xu Y., He HP, Qian PY, et al. Potent antifouling compounds produced by marine streptomyces. Bioresource Technology. 2010,101(4):1331- 1336), the test compound's anti-barnacle larval attachment activity. Adult barnacles (Darwin) were collected from the intertidal zone of Hong Kong (22°19'N, 114°16'E). Put 8 L of filtered seawater in a 12 L polystyrene plastic culture container, then put the adult barnacles into the container and place it to release the larvae. After 2.5 hours, the larvae are collected. The larvae at this stage are called no knots. The larva (nauplius) has no attachment ability. The nauplius larvae were placed in a container with 8 ...

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Abstract

The invention discloses an application for isobenzofuran type compounds in marine biofouling prevention and a preparation method thereof, which belongs to the field of marine fouling organism repellency and mainly solves the problem of marine biofouling. The application for isobenzofuran type compounds in marine biofouling prevention can be used for preventing underwater structure surfaces from being attached and / or fouled by marine organisms. The sobenzofuran type compounds are all obtained from isolation of fungus which is named cephalosporium cordaAL 031 in the strain classification, and the preservation serial number of the fungus strain is CCTCC Number M2010140. Extracts of secondary metabolites of the fungus and two isobenzofuran type compounds are performed a fouling resistance activity screening, the experiment result indicates that the extracts of secondary metabolites of the fungus and two isobenzofuran compounds show high fouling resistance activity, attachment of marine fouling organisms can be inhibited, and the application for isobenzofuran type compounds in marine biofouling prevention and the preparation method thereof can be used for prevention and treatment of marine biofouling and have a good application perspective.

Description

technical field [0001] The present invention relates to the field of marine fouling biological repellence, in particular to the application of isobenzofuran compounds isolated from secondary metabolites of fungi of the genus Cephalosporin AL031 in preventing marine biofouling and two kinds of isobenzofuran compounds Compound preparation methods. Background technique [0002] Biofouling refers to the accumulation of marine organisms such as algae, crustaceans, etc. anywhere on the hull, including the hull, rudder, propeller and other hull accessories, or seawater systems in the ship such as seawater suction tanks, engine cooling lines, shipboard and accessories Bioaccumulation and fouling of equipment, or mobile or fixed installations such as aquaculture facilities. In recent years, with the development of shipping, coastal defense, aquaculture and seaside power plants, the harm caused by marine biofouling has become more and more serious. At present, the fastest developing...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D307/87C07D307/88A01P1/00A01P7/04A01P17/00A01P7/00C12P17/04C09D5/16C12R1/745
Inventor 黄相中田凯郭俊明张英杰关小丽朱芸张润芝
Owner YUNNAN MINZU UNIV
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