Application of a kind of aporphina alkaloid
A kind of technology of alkaloid and Aporphi, which is applied in the field of application of Aporphylloid alkaloids, can solve the problems of low efficiency, pollution, difficulty in using marine antifouling coatings, etc., and achieve high safety, mature technology and strong inhibition Effects of biofouling activity
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preparation example Construction
[0051] The present invention also provides a preparation method of the above-mentioned aporphine alkaloids. The method comprises preparing said compound from a plant of the genus Ivy, such as Ivy cordifolia. Preferably, the aporphine alkaloids are prepared from the roots, stems, leaves and fruits of Ivy cordifolia. Preferably, the method has the steps of:
[0052] (1) Crush the dried root of Ivy radiata to obtain root fragments of Ivy radiata;
[0053] (2) Soak the fragments of Ivy cordifolia root in a solvent to obtain an extract;
[0054] (3) Filter the extract and concentrate under reduced pressure to obtain the extract;
[0055] (4) Disperse the extract in water, sequentially extract with petroleum ether, ethyl acetate and n-butanol, and after the extract is distilled under reduced pressure, obtain petroleum ether extract, ethyl acetate extract, and n-butanol respectively. Butanol extract part of the extract;
[0056] (5) Part of the extract extracted with ethyl aceta...
Embodiment 1
[0064] Example 1 Inhibitory activity of the plant extract of Ivy cordifolia on the attachment of barnacle larvae
[0065] Source of material: Ivy with heart leaves was collected in Luquan, Yunnan, and was identified as Uraria clarkei (Clarke) Gagnep. The specimen is preserved in the Herbarium of the School of Medicine for Nationalities, Yunnan University for Nationalities.
[0066] The preparation of the extract of Ivy cordifolia: crush the dried root of Ivy cordifolia to obtain the root fragments of Ivy cordifolia root respectively; then extract the fragments of the root of Ivy cordifolia root with volume 90% ethanol reflux for 3 times, each time for 2 hours to extract solution; the extract of Ivy cordifolia was filtered separately and concentrated under reduced pressure with a rotary evaporator to obtain an extract for later use.
[0067] Using an experimental model of inhibition of barnacle Venus larvae attachment (see scientific literature: Xu Y., He H. P., Qian P. Y, et...
Embodiment 2
[0072] Embodiment 2 further test with Ivy
[0073] Further experiments were performed with the roots of the Ivy plant. This is not to imply that other plants of the Ivy genus are not active.
[0074] Respectively use 70% acetone / water, 80% ethanol / water and 90% methanol / water as extraction solvents by volume percentage, and repeat Example 1. The results are shown in Table 2. The experimental results show that 70% acetone extract of Ivy cordifolia root and 80% ethanol extract of Ivy cordifolia root obtained respectively with 70% acetone / water, 80% ethanol / water and 90% methanol / water as extraction solvents It has the same significant anti-biofouling activity as the 90% methanol extract of Ivy cordifolia root, so using different concentrations of acetone / water or ethanol / water or methanol / water as the extraction solvent can also obtain the anti-biofouling activity in Ivy cordifolia active ingredient.
[0075] Table 2 The inhibitory effect of different solvent extracts of Ivy...
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