Protein A immunoadsorbent material and preparation method thereof

Abstract

The invention discloses a protein A immunoadsorbing material for absorbing antibodies, which is a polymer material coupled with agarose gel and protein A, and the protein A is a recombinant protein A with cysteine; The protein A immunosorbent material is prepared through three steps of preparation of recombinant protein A, activation of agarose gel, and synthesis of the immunosorbent material; the protein A immunosorbent material of the present invention can be directly, efficiently and selectively adsorbed from body fluids Pathogenic antibodies related to autoimmune diseases can be safely performed in extracorporeal circulation, and the purpose of eliminating pathogenic antibodies in patients can be achieved by one-time perfusion.

Description

technical field [0001] The invention relates to the field of medical biological materials, in particular to a protein A immunoadsorbent material for absorbing antibodies and a preparation method thereof. Background technique [0002] Even today, with the advancement of medicine, there are still many difficult diseases that humans cannot overcome, and autoimmune diseases are one of them. The pathogenesis of autoimmune diseases is complex and can affect multiple organs and systems throughout the body. The traditional treatment methods are mainly glucocorticoids and cytotoxic drugs, and the therapeutic effect has been poor. Autoimmune diseases have become one of the major diseases that threaten human health and life. [0003] Autoimmune diseases are characterized by the detection of one or more high-titer autoantibodies in the patient's serum or other body fluids. Autoantibodies are an important basis for the diagnosis of autoimmune diseases. Each autoimmune disease is accompa...

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Problems solved by technology

[0006] At present, there are many patents on the synthesis method of protein A immunoadsorbent materials. Generally, the primary amino group on the molecular chain of protein A reacts with other groups to immobilize on the agarose gel matrix, because the chemical reactivity of the primary amino group is not high. , published literature and patent experimental data show that after the primary amino group on protein A reacts with the carrier, the content of protein A immobilized on the carrier is 3-10 mg / mL carrier, and the synthesized material adsorbs 10-25 mg antibody per ml , because the adsorption capacity of the adsorption column for antibodies is not high, and one-time perfusion cannot achieve the purpose of removing pathogenic antibodies, the adsorption column must be equipped with CITEM10 system, which hinders the wide application of protein A immunoadsorption therapy in clinical practice

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