Anti-plant pathogenic fungi composition and its preparation method
The technology of plant pathogenic fungi and composition, which is applied in the field of pesticides, can solve the problems of affecting the quality of tobacco leaves, sunken spots, human health hazards, etc., and achieve the effects of significant antibacterial effect and high inhibition rate.
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Embodiment 1
[0031] Example 1 Preparation of Antifungal Composition Raw Materials
[0032] 2 kg of celery (Apium Graveolens L.) seeds were crushed, extracted 4 times with 10 times the volume of 95% ethanol, evaporated to remove the ethanol, the extract was shaken with water to form a suspension, extracted 4 times with petroleum ether to obtain 170 g of the extract.
Embodiment 2
[0033] Example 2 Component Analysis of Antifungal Composition Raw Materials
[0034] The composition analysis and determination of antifungal components were completed by gas chromatography-mass spectrometry (GC-MS). mm); (2) Analytical conditions and methods: the carrier gas is helium (99.999%), the flow rate is 1.0 ml / min, the injection volume is 1.0 microliters; the inlet temperature is 150 o C, keep for 2 min, and then 15 o C / min to 300 o C stabilized for 5 minutes; (3) Mass spectrometry conditions: ionization mode is EI source, ionization energy 70 electron volts, mass range (41-450) amu; (4) component analysis Replib, Mainlib spectral library comparison.
[0035] The peaks in the total ion chromatogram were scanned by mass spectrometry to obtain the mass spectrogram, searched and analyzed through the mass spectrogram library, and the relative percentage content of them in the fat-soluble components was obtained by using the peak area normalization method. The result...
Embodiment 3
[0039] Evaporate the solvent ethanol and extract with petroleum ether. The petroleum ether extract was chromatographed on a silica gel column, and eluted with a gradient of petroleum ether-ethyl acetate (100:0 – 0:100) solvent system; the specific low-polarity part was obtained, and the solvent was evaporated to dryness to obtain QCZ -28; GC-MS was used to analyze the active site (QCZ-28) obtained from the raw material, and the analysis results were as follows figure 1 and shown in Table 1.
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