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Kit for auxiliary diagnosis of tuberculosis patients

A technology for auxiliary diagnosis and tuberculosis, which is applied in measurement devices, instruments, scientific instruments, etc., can solve the problems of increasing the possibility of multidrug-resistant patients, delaying treatment, and failing to timely reflect the status of patients' disease outcomes, achieving good safety and efficiency. The effect of practicality

Inactive Publication Date: 2012-07-18
BEIJING TUBERCULOSIS & THORACIC TUMOR RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, the results of sputum culture usually can only be obtained after one month, so it cannot reflect the patient's condition in time.
However, the results of imaging examinations are greatly affected by subjective factors, and the diagnostic results of different doctors and different hospitals are quite different
If there is a deviation in the judgment of the progress of the disease, it will not only delay the treatment, but also increase the possibility of MDR patients

Method used

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  • Kit for auxiliary diagnosis of tuberculosis patients
  • Kit for auxiliary diagnosis of tuberculosis patients
  • Kit for auxiliary diagnosis of tuberculosis patients

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, the preparation of Mycobacterium tuberculosis total protein (MTB total protein)

[0029] 1. Use Middl ebrook 7H9 liquid medium to shake and culture H37Rv standard strain to OD 600 =1.

[0030] 2. Take 100mL of bacterial liquid, centrifuge at 3000g for 10min at 4°C, collect the bacterial cells, wash twice with PBS buffer, and then resuspend with 1mL of 4°C pre-cooled PBS buffer.

[0031] 3. Add 1 tube of 0.1mm silica beads (purchased from MP Biochemicals), and break it on the FastPrep-24 instrument (purchased from MP Biochemicals) at a shaking speed of 6.5m / s (meter / second) for 1 minute, 4°C, Centrifuge at 10000g for 10min, and take the supernatant.

[0032]4. Filter the supernatant with a 0.22 μm sterile filter to obtain a sterile MTB total protein solution.

[0033] 5. Use the BCA method to quantify the protein concentration of the sterile MTB total protein solution, and dilute it with sterile PBS buffer to a protein concentration of 1 mg / mL, which is ...

Embodiment 2

[0034] Example 2, the difference of IL-8 content in serum of patients with different degrees of bacteriological examination severity

[0035] It is generally believed that a positive sputum bacteria indicates that the patient excretes bacteria, is contagious, and the condition is more serious, while a negative sputum bacteria indicates that the patient does not excrete bacteria, is not infectious, and the condition is relatively mild. The experimental samples were: 17 tuberculosis patients with positive sputum bacteria (group 1), 32 tuberculosis patients with negative sputum bacteria (group 2) and 22 healthy people (without tuberculosis; group 3). Groups 1 and 2 are both hospital-diagnosed tuberculosis patients. The criteria for dividing sputum positive and sputum negative are as follows: Mycobacterium tuberculosis is isolated and cultured from the patient's sputum sample. If Mycobacterium tuberculosis can be obtained as Sputum positive, if Mycobacterium tuberculosis cannot be...

Embodiment 3

[0056] Example 3. Differences in IL-8 levels in serum of patients with different degrees of severity in imaging examinations

[0057] It is generally believed that the condition of patients with cavitation in lung lesions is more serious, and the condition of patients with lung lesions mainly nodules is milder. The experimental samples were: 23 tuberculosis patients with cavities in the lungs (the first group, referred to as patients with empty lungs), 29 tuberculosis patients with mainly tuberculosis nodules in the lungs (group two, referred to as patients with pulmonary nodules) ) and 22 healthy people (no tuberculosis; third group).

[0058] Each experimental sample was carried out as follows:

[0059] 1. Blood collection and antigen stimulation

[0060] Same as Step 1 of Example 2.

[0061] 2. Detection of IL-8 content

[0062] Same as Step 2 of Example 2.

[0063] Standard curve see image 3 , the standard curve equation is: y=112.99x-5.9424 (R 2 = 0.9992).

[006...

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Abstract

The invention discloses a kit for auxiliary diagnosis of tuberculosis patients. The kit comprises total protein of Mycobacterium tuberculosis and a substance for detecting interleukin-8. After the peripheral blood of patients to be tested is induced by using the total protein of the Mycobacterium tuberculosis, the expression levels of the interleukin-8 (IL-8) are remarkably different between tuberculosis patients and healthy person, among tuberculosis patients with different degrees of severity, and among different disease course stages of the same tuberculosis patient; and the kit can be used for the auxiliary diagnosis of the tuberculosis patients through the difference of the expression levels (the diagnosis comprises the diagnosis of the tuberculosis patients with different degrees of severity, the diagnosis of the different disease course stages of the same tuberculosis patient, and the diagnosis for cure of the tuberculosis patients).

Description

technical field [0001] The invention relates to a kit for auxiliary diagnosis of tuberculosis patients. Background technique [0002] Tuberculosis is an infectious disease that seriously threatens human health. One-third of the world's population is infected with Mycobacterium tuberculosis (MTB), and 2 million people die from tuberculosis every year. The death rate is only the lowest among infectious diseases. Ranked second to AIDS. [0003] The treatment cycle for tuberculosis is very long, generally more than 6 months, and drug side effects often occur during anti-tuberculosis chemotherapy, which makes patient compliance not optimistic. In particular, there are no good indicators for the assessment of disease progression during treatment and the assessment of cure status after treatment. There are many shortcomings in the bacteriological and imaging methods commonly used in clinical practice. The sensitivity of the most common sputum smear and sputum culture in bacterio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/531
Inventor 车南颖李传友张旭霞
Owner BEIJING TUBERCULOSIS & THORACIC TUMOR RES INST
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