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Cysteine protease inhibitor C test kit

A cysteine ​​protease and detection kit technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of antibody shedding, poor stability of sensitized latex particles, poor physical adsorption effect, etc., and achieve high accuracy , strong anti-interference ability and low production cost

Active Publication Date: 2014-01-01
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The latex-enhanced immunoturbidimetric method can label the polyclonal antibody on polystyrene latex particles without carboxyl group by physical adsorption method, but the sensitized latex particles obtained by physical adsorption method have poor stability, and the physical adsorption The effect is not good, it is easy to cause the antibody to fall off the surface of the latex particle

Method used

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  • Cysteine protease inhibitor C test kit
  • Cysteine protease inhibitor C test kit
  • Cysteine protease inhibitor C test kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Cystatin C detection kit standard preparation and assay method

[0029] The kit of the present invention relates to the main raw materials of reagents as follows:

[0030] 1. The rabbit anti-human cystatin C polyclonal antibody produced by our company according to the conventional method has a titer of 1:100000 by indirect ELISA after affinity purification, which meets the requirements of this reagent. The antibody has good specificity and no cross-reaction with other antigens.

[0031] 2. The present invention only uses two kinds of polystyrene latex particles with a diameter of 100-190nm and a carboxyl group of 20-60nm to carry out experiments. The corresponding detection dual wavelength has a main wavelength of 546nm and a secondary wavelength of 700nm.

[0032] 3. Recombinant human cystatin C protein is made by our company, expressed in Escherichia coli, purified by affinity and ion exchange, the cystatin C protein has high purity and good stability, and...

Embodiment 2

[0038] Example 2 Cystatin C detection kit performance index test

[0039] 1. Sensitivity test

[0040] Measure the sample of 6 kinds of different cystatin C contents, each sample measures 10 times, by calculating mean value and SD value (standard deviation value), as seen from Table 1, the sensitivity of detection kit of the present invention is 0.08mg / L.

[0041] Table 1

[0042] CYS-C (mg / L)

Measurement times

average

2.6SD

M+2.6SD

M-2.6SD

0

10

0.02

0.031

0.05

-0.02

0.04

10

0.05

0.044

0.10

0.01

0.08

10

0.10

0.031

0.13

0.07

0.12

10

0.15

0.058

0.21

0.10

0.16

10

0.19

0.044

0.23

0.14

0.20

10

0.22

0.032

0.25

0.19

[0043] 2. High value linear determination

[0044] Add recombinant cystatin C protein to blank ser...

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Abstract

The invention provides a cysteine protease inhibitor C test kit, wherein the kit comprises a reagent R1 and a reagent R2, the reagent R2 is a reaction solution for promoting specific binding of an antigen and an antibody, the reagent R2 is a polystyrene latex particle mixture coated by an anti-human cystatin C antibody and is put in a proper buffer solution. The polystyrene latex particle mixture in the reagent R2 is a mixture of large-diameter polystyrene latex particles and small-diameter polystyrene latex particles. The kit is high in detection sensitivity, high in accuracy, good in linearity within the test range, good in stability and low in production cost.

Description

technical field [0001] The invention relates to a medical detection kit, in particular to a kit for measuring cystine protease inhibitor C in human serum by using latex-enhanced immune turbidimetry. Background technique [0002] Cystatin C (cys-c, molecular weight 13kD), referred to as cystatin C, is a member of the cysteine ​​protease inhibitor family. Cystatin C is produced by almost all nucleated cells in human tissues and organs. Due to the small molecular weight of this protein, in the normal kidney, it can be freely filtered out through the glomerular mesangium, and then almost completely reabsorbed and broken down by the renal proximal tubular epithelial cells. Therefore, the glomerular filtration rate can be reflected by the concentration of cystatin C in the blood. Moreover, the fact that cystatin C serum concentrations are not affected by the course of allergies, sex, age, and muscle weight makes it a better choice than serum creatinine and beta 2 - Microglobuli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/545
Inventor 邹炳德邹继华方亮
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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