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Method for simultaneously detecting mercury ion and/or silver ion in water solution based on fluorescence resonance energy transfer

A fluorescence resonance energy and aqueous solution technology, which is applied in the fields of fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of heavy workload, high cost, and complicated process, and achieve the effect of low cost, small workload, and uncomplicated process

Active Publication Date: 2012-07-18
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional detection methods such as cold atomic fluorescence spectrometry (CV-AFS), cold atomic absorption spectrometry (CV-AAS), and inductively coupled plasma mass spectrometry (ICPMS) have many defects: heavy workload, high cost and complicated process
However, these sensors mainly focus on the detection of one ion, and fail to detect both ions at the same time.

Method used

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  • Method for simultaneously detecting mercury ion and/or silver ion in water solution based on fluorescence resonance energy transfer
  • Method for simultaneously detecting mercury ion and/or silver ion in water solution based on fluorescence resonance energy transfer
  • Method for simultaneously detecting mercury ion and/or silver ion in water solution based on fluorescence resonance energy transfer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Preparation of CdTe quantum dots coated with mercaptopropionic acid MPA

[0035] 0.189 g (5 mmol) of sodium borohydride NaBH 4 Dissolve in a glass vial containing 2.0mL ultrapure water, add 0.1528g (1.175mmol) tellurium Te powder, leave a small hole in the cap, and then place the reaction vessel in an ice-water bath at 0°C for reaction. After 12 hours, the black Te powder disappeared, and a clear NaHTe solution was obtained for future use. Weigh 0.57g (2.5mmol) CdCl 2 2.5H 2 Dissolve O in 150mL water, add 500μL (5.74mmol) of MPA dropwise under vigorous stirring, adjust the pH to 11.20 with 0.1mol / L NaOH solution, pass N 2 After deoxygenation and protection for 30 min, the NaHTe solution prepared above was quickly added, and the precursor solution of CdTe quantum dots was obtained after the reaction. Take 40mL of the precursor solution and add it to a stainless steel reaction kettle lined with polytetrafluoroethylene, and react at 160°C for 20 minutes to obtain t...

Embodiment 2

[0050] (1) Preparation of CdTe quantum dots coated with mercaptopropionic acid MPA.

[0051] The method is as described in Example 1.

[0052] (2) Design and synthesis of nucleic acid probes for detection

[0053] The method is as described in Example 1.

[0054] (3) Preparation of fluorescent probes for quantum dots and nucleic acids (CdTe-DNA)

[0055] The method is as described in Example 1.

[0056] (4) Hg 2+ detection

[0057] The assay system was as described in Example 1, containing various concentrations (0 to 50 nM) of Hg 2+ The aqueous solution of ions was added to the above detection system, and maintained at 25°C for 0.5h, so that the Hg 2+ The induced hybridization reaction between TAMRA-DNA and DNA2 proceeded completely. The fluorescence emission spectrum of the whole system is then measured.

Embodiment 3

[0059] (1) Preparation of CdTe quantum dots coated with mercaptopropionic acid MPA.

[0060] The method is as described in Example 1.

[0061] (2) Design and synthesis of nucleic acid probes for detection

[0062] The method is as described in Example 1.

[0063] (3) Preparation of fluorescent probes for quantum dots and nucleic acids (CdTe-DNA)

[0064] The method is as described in Example 1.

[0065] (4) Ag at the same time + and Hg 2+ detection

[0066] The detection system was as described in Example 1, containing different concentrations (0 to 50 nM) of Ag + and Hg 2+ The aqueous solution was added to the above detection system, and maintained at 25°C for 0.5h, so that the Hg 2+ Induced TAMRA-DNA hybridization reaction with DNA2 and Ag + The induced hybridization reaction between Cy5-DNA and DNA2 proceeded completely. The fluorescence emission spectrum of the whole system is then measured.

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Abstract

The invention relates to a method for simultaneously detecting a divalent mercury ion and a silver ion in water solution based on fluorescence resonance energy transfer, belonging to the field of heavy metal ion detecting technology. The method comprises the following steps: firstly, preparing a detecting probe, comprising following steps: (1), preparing a cadmium telluride (CdTe) quantum dot which is encapsulated by mercaptopropionic acid (MPA); (2), designing and composing a nucleic acid probe for detection; (3), preparing a fluorescent probe CdTe-DNA of quantum dots and nucleic acid; and secondly, detecting heavy metal ion Ag+ and / or Hg2+. The method provided by the invention can detect two ions simultaneously, the workload is low, the cost is low and the process is not complex.

Description

technical field [0001] The invention relates to a method for detecting divalent mercury ions and silver ions in aqueous solution based on fluorescence resonance energy transfer, belonging to the technical field of detection of heavy metal ions. Background technique [0002] Heavy metal ions such as mercury ions and / or silver ions are serious environmental pollutants. They pollute water and soil, pose a serious threat to human health and have severe toxic effects on flora and fauna. Mercury ions are enriched in the environment and prolonged exposure can cause lasting and severe damage to the central nervous system. Silver ions have been shown to be highly toxic to aquatic organisms. Therefore, it is very important to develop a method that can detect these two ions efficiently and sensitively. Traditional detection methods such as cold atomic fluorescence spectrometry (CV-AFS), cold atomic absorption spectrometry (CV-AAS), and inductively coupled plasma mass spectrometry (I...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 胥传来郝昌龙徐丽广许宙赵媛严文静
Owner JIANGNAN UNIV
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