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Method for extracting and purifying macromolecular active ingredients of plant

A technology for active ingredients and macromolecules, applied in the field of extracting and purifying plant macromolecular active ingredients, can solve the problems of loss of efficacy, inactivation of strong acid and alkali, residual organic solvents, etc., to achieve the effect of improving efficacy and maintaining activity

Active Publication Date: 2012-07-18
上海善力健生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these traditional methods all have the problems of high temperature, organic solvent residue and strong acid and alkali.
These factors will affect the structure of plant macromolecules, especially when the three-dimensional structure of the macromolecules is destroyed, its efficacy will be rapidly lost and inactivated, thus affecting the efficacy of the product

Method used

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  • Method for extracting and purifying macromolecular active ingredients of plant

Examples

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Comparison scheme
Effect test

Embodiment 1

[0020] A method for extracting and purifying macromolecular active ingredient glycoprotein in Lycium barbarum, the method comprises the following steps:

[0021] (1) Extracting Lycium barbarum with water to obtain an extract;

[0022] (2) The extract is clarified and filtered to obtain a clear liquid;

[0023] (3) The clear liquid is finely filtered through a microporous membrane with a pore size of 0.1-0.2 μm to obtain a fine filtrate;

[0024] (4) Since the molecular weight of Lycium barbarum glycoprotein is in the range of 70k-80k, the ultrafiltration membranes with molecular weights of 50k and 100k are selected for separation and purification to obtain the purified concentrate of Lycium barbarum glycoprotein;

[0025] (5) The purified concentrate was dried at low temperature to obtain Lycium barbarum glycoprotein.

[0026] Product Lycium barbarum glycoprotein of the present invention is carried out activity comparison result with the similar product sold on the market as...

Embodiment 2

[0030] A method for extracting and purifying macromolecular active ingredient tea polysaccharides in tea leaves, the method comprising the following steps:

[0031] (1) extracting the thick old tea with water to obtain an extract;

[0032] (2) The extract is clarified and filtered to obtain a clear liquid;

[0033] (3) The clear liquid is finely filtered through a microporous membrane with a pore size of 0.1-0.2 μm to obtain a fine filtrate;

[0034] (4) Since the molecular weight of the tea polysaccharide is about 40k, an ultrafiltration membrane with a molecular weight of 30k and 50k is selected for separation and purification to obtain a tea polysaccharide purification concentrate;

[0035] (5) The purified concentrated solution is dried at low temperature to obtain the active tea polysaccharide.

[0036] The active tea polysaccharide 10mg / kg prepared by the method of the present invention is compared with the commercially available similar product 500mg / kg in hypoglycemi...

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Abstract

The invention relates to a method for extracting and purifying macromolecular active ingredients of a plant. The method comprises the following steps of: (1) extracting a plant medicinal material to obtain extract; (2) clarifying and filtering the extract to obtain clear liquid; (3) performing fine filtration on the clear liquid by using a microporous membrane to obtain fine filtrate; (4) performing active molecule purification and interception on the fine filtrate to obtain macromolecular purified concentrate; and (5) drying the purified concentrate at a low temperature, and thus obtaining the macromolecular active ingredients. Compared with the prior art, the method has the advantages of greatly improving the effect of a product and the like.

Description

technical field [0001] The invention relates to a method for extracting and purifying plant macromolecular active ingredients. Background technique [0002] With the deepening of phytochemical research, plant macromolecular active substances have attracted more and more attention from the medical care community. Plant macromolecules include proteins, glycoproteins, glycopeptides, polysaccharides, polypeptides, enzymes and polyphenols. Studies have shown that plant macromolecules have strong biological activities, such as: immune regulation, anti-cancer, lowering blood sugar, lowering blood fat, lowering blood pressure, anti-oxidation and other effects. Therefore, in the R&D and production of natural herbal medicines, the use of plant macromolecules is becoming more and more extensive. [0003] The traditional preparation methods for plant macromolecules include: water extraction and alcohol precipitation method, organic solvent method, resin adsorption method, etc. Howeve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K1/34C08B37/00
Inventor 许宏华韩超
Owner 上海善力健生物科技有限公司
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