Domestic garbage harmful microorganism elimination deodorization liquid and preparation method thereof
A technology for domestic garbage and deodorizing liquid, which is applied in the field of domestic garbage elimination and deodorizing liquid and its preparation, can solve the problems that the inhibition of harmful microorganisms and the deodorization effect is not significant and rapid, and has not been popularized and applied, so as to achieve the elimination of harmful bacteria. source, low cost, and the effect of fresh ambient air
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Embodiment 1
[0040] (1) Activation and multiplication:
[0041] A. Cultivation of photosynthetic bacteria: inoculate the original bacteria of red bacillus into a liquid test tube with a cover, and cultivate under light at 30°C for 3 to 5 days to obtain a bacterial suspension; take 1% of the bacterial suspension and put it into a conical flask for cultivation. The base is the modified paradigm medium (sterilized at 121°C for 30 minutes), and then the secondary culture is shaken at 30°C and 120r / min, and the secondary culture of Rhodobacter erythrobacter is obtained after 48-72 hours of cultivation.
[0042] Cultivation of Bacillus: put the original strain of Bacillus subtilis on the nutrient agar medium, and cultivate it on a slant surface at 36°C for 24 hours, then inoculate it into a nutrient broth medium for shaking secondary culture, at a temperature of 36°C, 120r / min, after culturing for 48-72 hours, a secondary culture of Bacillus was obtained.
[0043] C Cultivation of lactic acid ...
Embodiment 2
[0057] (1) Activation and multiplication:
[0058] A. The cultivation of photosynthetic bacteria: Inoculate the original bacteria of green sulfur bacteria into a liquid test tube with a cover, and cultivate under light at 31°C for 3 to 5 days to obtain a bacterial suspension; take 1% of the bacterial suspension and put it into a triangular flask, The culture medium is an improved culture medium (sterilized at 121°C for 30 minutes), and then undergoes shaking secondary culture at a temperature of 31°C and 120 r / min. After culturing for 48-72 hours, a secondary culture of green sulfur bacteria is obtained.
[0059] Cultivation of Bacillus bacillus: the original strain of Bacillus licheniformis was placed on the beef extract peptone medium, and cultured on a slant for the first level at 32°C, and then inoculated into the same liquid corn steep liquor medium for shaking secondary culture, the temperature was 32°C, 120r / min, the secondary culture of Bacillus licheniformis was obta...
Embodiment 3
[0074] (1) live and multiply
[0075] A Cultivation of photosynthetic bacteria: inoculate the original purple non-sulfur bacteria into a liquid test tube with a cover, and light
[0076] Cultivate for 3-5 days to obtain the bacterial suspension; take 1% of the bacterial suspension and put it into the Erlenmeyer flask. , temperature 31°C, 120r / min, cultured for 48-72 hours to obtain a secondary culture of purple non-sulfur bacteria.
[0077] B. Bacillus culture: the original strain of Bacillus cereus was placed on beef extract peptone medium, and cultured on a slant for the first level at 32°C, and then inoculated into the same liquid corn steep liquor medium for shaking secondary culture, at a temperature of 32°C. 120r / min, cultured for 48-72 hours to get the secondary culture of Bacillus cereus.
[0078] C Cultivation of lactic acid bacteria: Place the original strains of Bifidobacterium on the wort medium, do primary slope culture at 31°C, then inoculate into the same liqu...
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