Quantitative polymerase chain reaction (PCR) microfluidic chip integrated device for integrated electrochemical detection technology

Abstract

The invention discloses a quantitative polymerase chain reaction (PCR) microfluidic chip integrated device for an integrated electrochemical detection technology. The PCR microfluidic chip integrated device comprises a fixed foundation, thermostats, a PCR microfluidic chip, and an electrochemical detector, and is characterized in that: three independent thermostats, namely a low-temperature annealing thermostat, an appropriate-temperature extending thermostat, and a high-temperature denaturation thermostat are sequentially manufactured on the fixed foundation in parallel; PCR reaction in the PCR microfluidic chip can be finished in a low-temperature annealing area, an appropriate-temperature extending area and a high-temperature denaturation area; and the electrochemical detector for quantitative detection of PCR reactant is arranged in a deoxyribonucleic acid (DNA) amplification reaction tube. The quantitative PCR microfluidic chip device is suitable for various gene diagnosis and detection and laboratories. The device platform integrates temperature control, PCR reaction and detection, and is high in miniaturization degree.

Description

technical field [0001] The invention belongs to a quantitative PCR microfluidic chip integration device integrating electrochemical detection, specifically, the electrochemical detection technology and PCR technology are integrated in the microfluidic chip system, and the advantages of integration and multi-function are exerted , the microfluidic chip is suitable for various gene diagnosis detection and laboratory use, and belongs to the field of biological detection technology. Background technique [0002] Polymerase chain reaction (full name in English: Polymerase Chain Reaction, referred to as PCR) is a method for enzymatically synthesizing specific DNA fragments in vitro, which has the characteristics of strong specificity, high sensitivity, easy operation, and time saving. PCR consists of three basic reaction steps of high-temperature denaturation, low-temperature annealing (refolding) and temperature extension, and a cycle is performed for multiple cycles. It only ta...

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Problems solved by technology

At present, the integrated PCR microfluidic chip technology is generally only designed to complete the PCR technology in the microfluidic chip. The PCR microfluidic chip is an existing technology, but it lacks real-time quantitative detection technology, which limits the application of the integrated PCR microfluidic chip. Application range

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