Application of tanshinone I to treatment of microglia-mediated disease
A technology of microglia and tanshinone, applied in the field of tanshinone I, can solve the problems of decreased curative effect, toxic and side effects, etc.
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Embodiment 1
[0064] Example 1: Effect of Tanshinone I on Microglia Activation-Induced Cell Death (AICD)
[0065] BV-2 cells were pre-incubated with tanshinone I (1, 5, 10, 20 μmol / L) for 0.5 h, and then stimulated with LPS (lipopolysaccharide, 0.1 μg / mL). After culturing for 24 hours, discard the supernatant, add CCK-8 (cell counting reagent, Guangzhou Yanchuang Biotechnology Co., Ltd.), continue to incubate for 30 minutes, and measure the absorbance at a wavelength of 450 nm. The experiment also set up the control group (normally growing cells), the model group (LPS) and the positive control group "Mino+LPS" group (5 μmol / L minocycline+lipopolysaccharide) in parallel. see results figure 1 .
[0066] The results showed that LPS can cause activation-induced cell death and significantly reduce the viability of BV-2 cells. Tanshinone I can inhibit LPS-induced activation-induced cell death in a dose-dependent manner.
[0067] In addition, "extract A" and "extract B" were tested as reagen...
Embodiment 2
[0068] Example 2: Effect of Tanshinone I on Viability of Microglial Cells
[0069] After BV-2 cells were cultured with tanshinone I (1, 5, 10, 20 μmol / L) for 24 hours, discard the supernatant, add CCK-8, continue to incubate for 30 minutes, and measure the absorbance at a wavelength of 450 nm. In the experiment, the control group (cells with normal growth) and the "Mino" group were set up in parallel. see results figure 2 .
[0070] The results showed that tanshinone I could not inhibit cell viability and did not cause cell death.
Embodiment 3
[0071] Example 3: Effect of Tanshinone I on Secretion of Inflammatory Mediators by Microglia
[0072] The mouse microglial cell line BV-2 was cultured in complete DMEM medium. Inoculate into 48-well culture plate overnight, add different concentrations of Tanshinone I (final concentration 1, 5, 10, 20 μmol / L) for pre-incubation for 0.5 h, and then add endotoxin (LPS, final concentration 0.1 μg / mL) for stimulation . Experimental groups: normal control group, LPS group, Tanshinone I plus LPS group, and "Mino+LPS" group. The concentration of NO was detected by Greiss method, and the concentration of TNF-α was measured by Elisa kit. see results image 3 .
[0073] The results showed that Tanshinone I could significantly inhibit the release of NO and TNF-α induced by LPS in a dose-dependent manner within the concentration range of 1-20 μmol / L.
[0074] In addition, "extract A" and "extract B" were tested as reagents, and the two extracts showed the same dose as the pure tans...
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