Preparation method and application of sensing chip for detecting atrazine
A sensor chip and atlafeng technology, which is applied in the field of sensor chip preparation, can solve the problems of complex and time-consuming detection of atlafeng, a large number of detection samples, and difficulty in carrying, etc., and achieves high sensitivity and sample size. Less, the effect of simple and convenient operation
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Embodiment 1
[0031] (1) On the glass substrate, the gold film of 50nm is vacuum-evaporated to make a surface plasmon resonance sensor chip, and the amount of substance concentration that is put into the sensor chip is the ethanol of dodecyl mercaptan of 1mmol / L Soak in the solution for 24 hours, self-assemble monomolecular alkyl chain layer on the gold film, then take it out, rinse with ethanol, blow dry with nitrogen gas and set aside.
[0032] (2) Dissolve 0.1mmol of the template molecule Atla Feng and 0.3mmol of the functional monomer methacrylic acid (MAA) in 4ml of the porogen acetonitrile under the condition of avoiding light, mix them uniformly by ultrasonic mixing for 50min, and let stand for 1h; then add 0.4 mmol of crosslinking agent ethylene glycol dimethacrylate (EDGMA) and 0.08 mmol of initiator benzophenone were ultrasonically mixed for 4 minutes to mix uniformly, and then nitrogen gas was passed for 10 minutes to obtain a polymerization reaction liquid.
[0033] (3) Inject t...
Embodiment 2
[0041] (1) A 50nm gold film is vacuum-evaporated on a glass substrate to make a surface plasmon resonance sensor chip, and the sensor chip is put into a concentration of 1mmol / L dodecyl mercaptan and soaked in an ethanol solution After 48 hours, a monomolecular alkyl chain layer was self-assembled on the gold film, then removed, rinsed with ethanol, and dried with nitrogen gas for later use.
[0042] (2) Dissolve 0.1mmol of the template molecule Atla Feng and 0.5mmol of the functional monomer methacrylic acid in 4ml of the porogen acetonitrile under the condition of avoiding light, mix ultrasonically for 50min, and let it stand for 1h; then add 1.2mmol of the crosslinking agent Ethylene glycol dimethacrylate and 0.08 mmol initiator benzophenone were ultrasonically mixed for 4 minutes, and then nitrogen gas was passed for 10 minutes to obtain a polymerization reaction solution.
[0043] (3) Inject the polymerization reaction solution obtained in step (2) into the reaction tank,...
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