Traditional Chinese medicine venenum bufonis extract and preparation method thereof

Abstract

The invention relates to a traditional Chinese medicine venenum bufonis extract. A method for extracting the extract includes steps of extracting traditional Chinese venenum bufonis medical materials at the temperature ranging from 60 DEG C to 90 DEG C by ethanol water with volume percent of 60-95% in a backflow manner, separating extract liquid via silicagel columns after the extract liquid is concentrated, adopting petroleum ether and acetone with volume ratio of 1-8:1 as eluant to realize gradient elution, collecting parts of eluent, and filtering, concentrating and drying the eluent in a pressure reduction manner to obtain the traditional Chinese medicine vanenum bufonis extract containing mixture of three types of bufotalin including resibufogenin, cinobufagin and bufalin, wherein the sum of the weight percentages (calculated when three types of the bufotalin are dried) of the three types of the bufotalin is higher than or equal to 95%. The invention further provides the preparation method for the traditional Chinese medicine venenum bufonis extract. The sum of the weight percentages of the three types of the bufotalin in the traditional Chinese medicine venenum bufonis extract is higher than 95%, extraction transfer rate is high, the quantity of used solvent is lower, residual solvent is little, pollution degree is lower, operation is simple and convenient, and industrial production is realized easily.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine extraction, and in particular relates to a traditional Chinese medicine toad venom extract and a preparation method thereof. Background technique [0002] The medicinal toad is Bufo bufogaraarizans Cantor or Bufo meLanostictus Schneider, which is an animal of the amphibian bufo family. Toad venom is the white slurry secreted by the auricular glands and skin glands of the giant bufo or black-framed toad, which is processed and dried. The application of traditional Chinese medicine toad venom has a history of thousands of years. The description of toad venom in "Compendium of Materia Medica" is as follows: toad venom travels through twelve meridians, Tibet, membranes, valleys, and joints. Toad venom is also a medicine for treating malnutrition accumulation, dispelling swelling and detoxifying furunculosis. It can dissolve all stagnant and stagnant diseases, such as accumulatio...

AI Technical Summary

Problems solved by technology

[0004] Chinese patent application "a kind of toad venom extract and preparation method thereof" (application number is 200710041880.7, publication number is CN101322723A, announcement date is December 17, 2008) uses dehydrated alcohol to extract, and extract is separated through silica gel column, with certain The method of isocratic elution with the ratio of petroleum ether and acetone obtains a mixture of three fat-soluble components of esterbufagenin, cinobufagin and bufafolin, the total amount of which is higher than 90%, but the method adopts an extraction solvent of Dehydrated alcohol, its extraction efficiency is lower, and the consumption of sherwood oil used for separation, acetone is bigger, and the degree of environmental pollution and destruction is also bigger; investigation, and the transfer rate has not been disclosed; more importantly, this method does not specify the drying temperature and time of the traditional Chinese medicine Bufoni extract, because organic solvents are used in the separation and purification process, which may lead to A large amount of toxic organic solvents such as petroleum ether and acetone remain, resulting in low content and greatly increased toxicity

Therefore, from the perspective of safety and effectiveness, the total content of the three bufagenins needs to be further increased, and the residual amount of organic solvents such as acetone and petroleum ether has not been checked and measured.

Images