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Quick and high-efficiency fluorescence detection method for phosphate ions

A technology of phosphate ion and detection method, applied in the field of analysis and detection, can solve the problems of quantum dot fluorescence quenching, false signal, etc., and achieve the effect of improving selectivity and avoiding the interference of fluorescence quenching

Inactive Publication Date: 2012-06-13
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, many factors in the solution can cause the fluorescence quenching of quantum dots, which can lead to false signals

Method used

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  • Quick and high-efficiency fluorescence detection method for phosphate ions
  • Quick and high-efficiency fluorescence detection method for phosphate ions
  • Quick and high-efficiency fluorescence detection method for phosphate ions

Examples

Experimental program
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Effect test

Embodiment 1

[0018] a, 0.013g of cysteine ​​hydrochloride and 80ml of 0.001M CdCl 2 After the solution was mixed, 0.1M NaOH solution was added to adjust the pH of the solution to 7.5;

[0019] b. In the above mixed solution, after 30 minutes of high-purity nitrogen gas was introduced, 20ml of 0.002M Na 2 S aqueous solution, continue to react under nitrogen stirring for 20 minutes to obtain water-soluble CdS nanomaterials;

[0020] c. Mix 0.25ml of the obtained water-soluble CdS nanomaterial with 0.65ml of 0.1M Tris-HCl buffer solution, add 0.5ml of 1.0×10 -4 mol / L lanthanum nitrate solution, after 1 minute of reaction, add standard solutions of phosphate ions to be measured or simulated water samples containing phosphate ions for fluorescence measurement.

Embodiment 2

[0022] a, 10mL 0.01mol / L thioglycolic acid solution and 80mL 0.001M Cd(ClO 4 ) 2 After the solutions were mixed, 0.1M NaOH solution was added to adjust the pH of the solution to 9.0;

[0023] b, in the above-mentioned mixed solution, after feeding high-purity nitrogen for 30 minutes, add 20ml of 0.002M NaHSe aqueous solution, and continue to react under nitrogen stirring for 20 minutes to obtain water-soluble CdSe nanomaterials;

[0024]c. Mix 0.25ml of the obtained water-soluble CdSe nanomaterial with 0.65ml of 0.1M Tris-HCl buffer solution, add 0.1ml of 1.0×10 -4 mol / L of cerium (III) sulfate solution, after 1 minute of reaction, add standard solutions of phosphate ions of different concentrations to perform fluorescence measurement.

Embodiment 3

[0026] a, 0.118g of trisodium citrate dihydrate and 80ml of 0.001M Cd(ClO 4 ) 2 After the solution was mixed, 0.1M NaOH solution was added to adjust the pH of the solution to 7.0;

[0027] b, in the above-mentioned mixed solution, after feeding high-purity nitrogen for 30 minutes, add 20ml of 0.002M NaHTe aqueous solution, and continue to react under nitrogen stirring for 20 minutes to obtain water-soluble CdTe nanomaterials;

[0028] c. Mix 0.25ml of the obtained water-soluble CdTe nanomaterial with 0.65ml of 0.1M Tris-HCl buffer solution, add 0.1ml of 1.0×10 -4 mol / L europium nitrate solution (using Eu 2 O 3 Add nitric acid to dissolve the aqueous solution and heat it to evaporate the nitric acid), after 1 minute of reaction, add 1.0 × 10 -5 1 mL of simulated water sample of mol / L phosphate ions was used for fluorescence measurement.

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Abstract

The invention provides a quick and high-efficiency fluorescence detection method for phosphate ions. Rare earth ions can quench fluorescence based on a quantum dot, and the fluorescence with the phosphate ions can be recovered effectively. Compared with the most common fluorescence quenching type probe based on the quantum dot, a novel off-on type quantum dot fluorescence probe can effectively prevent the fluorescence from being quenched due to other factors in a solution, so that the measurement selectivity is improved greatly. As known, the method is the first application of the quantum dot to measurement of the phosphate ions. According to the method, the phosphate ions can be measured with high sensitivity; the linear range is 1*10<-7> to 5*10<-6> mol / L; and the detection limit is 5*10<-8> mol / L. Common anions do not have fluorescence response signals, so that the method is high in selectivity; a satisfying result of measurement of the phosphate ions in an analog water sample is obtained; and the method is expected to be applied to detection of the phosphate ions in a water body.

Description

Technical field: [0001] The invention relates to the field of analysis and detection, in particular to a synthesis method of a water-soluble, high-stability quantum dot optical probe and its application in fluorescence detection of phosphate ions in water bodies. Background technique: [0002] Phosphate ions play a pivotal role in numerous processes involving life, environment and chemistry [P.D.Beer and E.J.Hayes, Coord.Chem.Rev., 2003, 240, 167-189.]. People's daily washing products and farmland fertilization will produce phosphate ion discharge, resulting in the increasing content of phosphate in natural water. However, the phosphorus content in the water is too high (> 0.2 mg L -1 ), it will enrich the water body nutrition, cause the excessive reproduction of algae and other aquatic plant pollution, cause the water quality to deteriorate, bring odor to the water body, and cause environmental pollution [H.P.Jarvie, C.Neal, P.J.A.Withers, A.Robinson and N. Salter, Hyd...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 王光丽焦焕军董玉明朱晓瑛
Owner JIANGNAN UNIV
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