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Identification method of special type pathogenicity of fusarium oxysporum sesame

A technology of Fusarium oxysporum and identification method, which is applied in the direction of biochemical equipment and method, botanical equipment and method, microbial determination/inspection, etc. Long period of bacterial infection and slow development of sesame radicle, etc., to achieve stable plant disease results, reliable pathogenicity determination, and accurate pathogenicity differences

Active Publication Date: 2014-06-25
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the weak research foundation of sesame diseases, there have been no literature reports on the indoor identification and evaluation methods of the pathogenicity of Fusarium oxysporum sesame-specific pathogenic bacteria so far, and the identification of resistance to sesame wilt at home and abroad is only carried out in field disease nurseries.
The method of using fungus cakes to infect sesame germinated seeds to identify the pathogenic bacteria of sesame wilt has been patented in my country, but due to the slow development of sesame radicle, the upright growth of plants is easily affected by the test conditions, the sustainable period of materials is short, and the inoculation is uneven, etc. oxysporum has the characteristics of long infection cycle and slow onset, this method is not suitable for identifying and evaluating the pathogenicity of Fusarium oxysporum sesame-specific pathogenic bacteria
In addition, due to factors such as the underdeveloped root system of sesame and the excessive impact of root injury treatment on plant growth, experiments have also proved that commonly used methods such as root irrigation, root injury inoculation, and radicle inoculation are not suitable for sesame wilt. The pathogenicity evaluation of pathogenic bacteria cannot be well used in the analysis of genetic diversity of pathogenic bacteria and the identification of physiological races

Method used

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  • Identification method of special type pathogenicity of fusarium oxysporum sesame
  • Identification method of special type pathogenicity of fusarium oxysporum sesame
  • Identification method of special type pathogenicity of fusarium oxysporum sesame

Examples

Experimental program
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Effect test

Embodiment 1

[0027] This example is the pathogenicity identification and evaluation method of Fusarium oxysporum sesame-specific strain No.HSFO 10012:

[0028] 1) Preparation of PDB medium: Weigh 200g of potatoes, wash, peel and cut into small pieces, add water and boil for 20-30min, until it can be pierced by a glass rod, filter it into a beaker with four layers of gauze, and add glucose 20g, make up water to 1000ml, stir and mix well, divide into Erlenmeyer flasks, and sterilize at 121°C for 20 minutes;

[0029] 2) Preparation of conidia suspension of No.HSFO 10012 strain: Take out the preserved No.HSFO10012 strain, pick a small amount of bacterial liquid and inoculate it into PDB liquid medium, shake and culture at 160r / min, 28°C for 3 days, and then use Filter through two layers of sterile gauze to prepare the No.HSFO 10012 strain conidia suspension, and prepare it with sterile water to a concentration of 1×10 6 Individual / mL conidia suspension, ready for inoculation;

[0030] 3) See...

Embodiment 2

[0036]Example 2 is the pathogenicity identification and evaluation method of Fusarium oxysporum sesame-specific strain No.HSFO 10008:

[0037] 1) Preparation of PDB medium: Weigh 200g of potatoes, wash, peel and cut into small pieces, add water and boil for 20-30min, until it can be pierced by a glass rod, filter it into a beaker with four layers of gauze, and add glucose 20g, make up water to 1000ml, stir and mix well, divide into Erlenmeyer flasks, and sterilize at 121°C for 20 minutes;

[0038] 2) Preparation of conidia suspension of No.HSFO 10008 strain: Take out the preserved No.HSFO10008 strain, pick a small amount of bacterial liquid and inoculate it into PDB liquid medium, shake and cultivate at 160r / min, 28°C for 3 days, and then use Filter through two layers of sterile gauze to prepare the No.HSFO 10008 strain conidia suspension, and prepare it with sterile water to a concentration of 1×10 6 Individual / mL conidia suspension, ready for inoculation;

[0039] 3) Seed ...

Embodiment 3

[0045] Embodiment 3 is the pathogenicity identification and evaluation method of Fusarium oxysporum sesame-specific strain No.HSFO 10012:

[0046] 1) Preparation of PDB medium: Weigh 200g of potatoes, wash, peel and cut into small pieces, add water and boil for 20-30min, until it can be pierced by a glass rod, filter it into a beaker with four layers of gauze, and add glucose 20g, make up water to 1000ml, stir and mix well, divide into Erlenmeyer flasks, and sterilize at 121°C for 20 minutes;

[0047] 2) Preparation of conidia suspension of No.HSFO08005 strain: Take out the preserved No.HSFO08005 strain, pick a small amount of bacterial liquid and inoculate it into PDB liquid medium, shake and cultivate at 160r / min, 28°C for 3 days, and then use Filter through two layers of sterile gauze to prepare the No.HSFO 08005 strain conidia suspension, and prepare it with sterile water to a concentration of 1×10 6 Individual / mL conidia suspension, ready for inoculation;

[0048] 3) Se...

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Abstract

The invention relates to an identification method of pathogenicity of fusarium oxysporum sesame special type bacterial strain to host sesame, which comprises the following steps: (1) pretreatment of seeds; (2) bacterial inoculation culture of the seeds: conidium suspending liquid is taken and poured into sterile vermiculite with the volume to be two times that of the conidium suspending liquid, vermiculite mixed with pathogenic bacteria and sterile soil are mixed evenly according to the volume ratio of 1:3 and charged in a split mode, sterile sesame seeds exposing white are sown in a paper bowl, 10 particles are evenly placed in each bowl, each variety is repeated and planted for three times, mixed soil for mixing bacteria is lightly covered on the surface of the seeds and placed into an artificial climate box for light and dark alternated culture for 12 hours per day at 25 DEG C and at the humidity of about 80%, and other culture conditions are the same with equal-volume sterile water as comparison; and (3) performing pathogenicity identification. The identification method is simple, convenient and easy to implement, pathogenicity of the pathogenic bacteria is stable, results are reliable, repeatability is good, and the fact that whether fusarium oxysporum sesame special type bacterial strain has pathogenicity on sesame seedlings and pathogenic strength of the fusarium oxysporum sesame special type bacterial strain can be accurately reflected.

Description

technical field [0001] The invention relates to a method for identifying and evaluating the pathogenicity of a sesame specialized strain of Fusarium oxysporum by using indoor paper pots to cultivate sesame seedlings, and belongs to the field of biotechnology. Background technique [0002] Fusarium oxysporum sesame-specialized type is a pathogenic fungus that causes blight of sesame and seriously harms the growth of sesame. In my country's sesame production, the annual incidence of Fusarium wilt caused by this fungus is about 15%, and it can reach more than 30% in severe cases, which has a great impact on yield and quality. In order to ascertain the pathogenic characteristics of the sesame-specific pathogen of Fusarium oxysporum, we carried out the collection of sesame-specific strains and established a database of the sesame-specific strains of Fusarium oxysporum in my country. The pathogenicity identification research of the main strains in the strain library is the key an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/08A01G31/00C12Q1/02
Inventor 苗红梅仇存璞魏利斌杜华张海洋
Owner HENAN ACAD OF AGRI SCI
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