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Preparation method of promoter for producing ceramide and/or glucosylceramide

A technology of ceramide and manufacturing method, applied in the field of ceramide and/or glucosylceramide production accelerator, to achieve the effect of improving moisturizing ability

Inactive Publication Date: 2014-03-19
KAO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, there is no report on the use of microorganisms of the genus Debaryomyces to produce ceramide and / or glucosylceramide production promoters

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] (Manufacture of ceramide and / or glucosylceramide production promoter)

[0051] Debaryomyces hansenii (purchased from Jiangnan University and deposited in CICIM, the Industrial Microbiological Resources and Information Center of Chinese Universities) is used as a microorganism for producing ceramide and / or glucosylceramide production promoter from soybean dregs. No. 476-20-1).

[0052] Inoculate the above-mentioned strain obtained after culturing in YPD (Yeast Extract Peptone Dextrose Medium) medium at 30°C into a Erlenmeyer flask (300 ml in capacity) containing 100 ml of the ceramide / glucosylceramide production promoter production medium, at 30°C Incubate for 72 hours. The medium contains 10w / v% okara and 1w / v% glucose. Among them, the bean dregs are prepared by immersing soybeans in water for 12 hours to allow the soybeans to fully absorb water, then grinding and crushing the absorbed soybeans, filtering and removing liquid components, and then draining the remaining resi...

Embodiment 2

[0056] Debaryomyces hansenii (purchased from Jiangnan University and deposited in CICIM, China University Industrial Microbiological Resources and Information Center, original number 514) is used as a microorganism for producing ceramide / glucosylceramide production promoter from soybean dregs. -20-4). The fermented soybean dregs extract was obtained by the same method as in Example 1, as the ceramide / glucosylceramide production promoter of the present invention.

[0057] Furthermore, the fermented soybean dregs extract was dissolved in 50 v / v% ethanol, and the concentration of the fermented soybean dregs extract was adjusted to 1 w / v%, and then stored at 4°C for subsequent ceramide / glucosylceramide production promotion tests.

Embodiment 3

[0059] Debaryomyces hansenii (purchased from Jiangnan University and deposited in CICIM, Industrial Microbiological Resources and Information Center of Chinese Universities, CICIM, deposit number CICIMY0356 ). The fermented soybean dregs extract was obtained by the same method as in Example 1, as the ceramide / glucosylceramide production promoter of the present invention.

[0060] Furthermore, the fermented soybean dregs extract was dissolved in 50 v / v% ethanol, and the concentration of the fermented soybean dregs extract was adjusted to 1 w / v%, and then stored at 4°C for subsequent ceramide / glucosylceramide production promotion tests.

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PUM

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Abstract

The invention provides a preparation method of a promoter for producing ceramide and / or glucosylceramide, wherein debaryomyces microbes are cultured in a culture medium containing bean dregs and saccharides, and the promoter for producing ceramide and / or glucosylceramide is obtained from the supernatant. According to the preparation method of the invention, special microbes are used, and bean dregs which are by products in food processing industry are used as a main raw material of the culture medium; therefore the promoter for producing ceramide and / or glucosylceramide is obtained with low cost and high efficiency.

Description

Technical field [0001] The present invention relates to a method for producing a ceramide and / or a glucosylceramide production promoter that can promote the production of ceramide and / or glucosylceramide in the skin by using specific microorganisms and using bean dregs, a by-product of the food processing industry, as the main raw material of the culture medium . Background technique [0002] Ceramide, one of sphingolipids, exists in the stratum corneum of the skin and accounts for about 50% of the intercellular lipids in the stratum corneum. Sphingolipid is a general term for complex lipids with a sphingosine skeleton. Glucosylceramide is a sphingolipid formed by combining glucose with ceramide. The ceramide synthesized by epidermal cells is temporarily stored in the form of glucosylceramide or sphingomyelin; then it is excreted outside the cell, in the presence of Glucocerebrosidase and Sphingomyelinase Under the action of ceramide, it becomes ceramide again, thus exerting i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02A61K8/99A61Q19/00C12R1/645
Inventor 孔凡旗
Owner KAO CORP
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