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Composite heterotrophic nitrifying bacterial agent and application of same in nitrogen removal treatment of waste water containing ammonia and nitrogen

A nitrifying bacteria agent and ammonia nitrogen technology, applied in the field of environmental microorganisms, can solve the problem of low ammonia nitrogen removal rate in ammonia nitrogen wastewater, achieve the effects of less organic carbon sources, convenient use, and lower total nitrogen

Active Publication Date: 2012-05-09
JIANGSU JIANGDA ECOLOGICAL SCI & TECHCO +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] The purpose of the present invention is to overcome the deficiencies in the prior art and provide a compound heterotrophic nitrifying bacteria agent, which can solve the problem of common biological denitrification under the condition of relatively low C / N. The problem of low ammonia nitrogen removal rate in high-concentration ammonia-nitrogen wastewater

Method used

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  • Composite heterotrophic nitrifying bacterial agent and application of same in nitrogen removal treatment of waste water containing ammonia and nitrogen
  • Composite heterotrophic nitrifying bacterial agent and application of same in nitrogen removal treatment of waste water containing ammonia and nitrogen
  • Composite heterotrophic nitrifying bacterial agent and application of same in nitrogen removal treatment of waste water containing ammonia and nitrogen

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Experimental program
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Effect test

Embodiment 1

[0040] Embodiment one: the preparation method of compound heterotrophic nitrifying bacteria agent, adopts the following steps:

[0041] (1) Preparation of domesticated bacteria liquid: uniformly mix activated sludge obtained from multiple sewage treatment plants, soil obtained from chemical fertilizer plants, and organic fertilizer obtained from organic fertilizer plants, and take 1 to 2 g of mixed soil and place it in 200 mL of domesticated culture In a 500mL Erlenmeyer flask based on the base, the domestication culture was carried out on a shaker at 20-35°C, and the rotating speed of the shaker was 180-200 rpm; the domestication culture process took 3-4 days as a cycle, and each cycle ended Afterwards, take 10mL of the domesticated bacteria liquid and place it in another 500mL conical flask, then add 190mL of fresh domestication medium to the conical flask to continue the domestication culture, and use ammonia reagent to detect the removal of ammonia nitrogen after each domes...

Embodiment 2

[0050] Embodiment two: the laboratory deammoniation nitrogen treatment of compound heterotrophic nitrifying bacteria agent, adopts the following steps:

[0051] The strains N4, N5, N6 and the domesticated bacteria liquid were respectively inserted into the heterotrophic nitrification medium for expansion culture. The pH value of the culture was 6, the temperature was 20°C, and the shaker speed was 120 rpm. After 24 hours of culture, it was cultivated The compound heterotrophic nitrifying bacteria agent is mixed according to the volume ratio of 1: 1: 1: 1; the above-mentioned compound heterotrophic nitrifying bacteria agent is inserted into the initial ammonia nitrogen concentration by 0.1% inoculum concentration is about 260mg / L, C / In the artificial wastewater with N=5.5, cultured for 48 hours under the conditions of 30°C and shaker speed of 180 rpm, the denitrification effect was regularly detected, and compared with the denitrification effect of the domesticated bacteria sol...

Embodiment 3

[0056] Embodiment three: the denitrification treatment of high-concentration ammonia nitrogen wastewater by compound heterotrophic nitrifying bacteria agent, adopts the following steps:

[0057] The above-mentioned strains N4, N5, N6 and the domesticated bacteria liquid were respectively inserted into the heterotrophic nitrification medium for expansion culture, the pH value of the culture was 9, the temperature was 30°C, and the shaker speed was 200 rpm. After 24 hours of culture, it was cultured The compound heterotrophic nitrifying bacteria agent is mixed according to the volume ratio of 1: 1: 1: 3; the above-mentioned compound heterotrophic nitrifying bacteria agent is inserted into the initial ammonia nitrogen concentration by about 450mg / L, C / N=3.2 in the artificial wastewater, cultured at 30°C, shaker speed 180 rpm, and regularly tested the denitrification effect, the results are as follows: image 3 as shown, image 3 The middle abscissa is time, the unit is hour, th...

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Abstract

The invention relates to a composite heterotrophic nitrifying bacterial agent. The composite heterotrophic nitrifying bacterial agent is characterized by comprising pseudomonas CCTCC M 2011416, pseudomonas CCTCC M 2011417 and arthrobacter CCTCC M 2011418. The composite heterotrophic nitrifying bacterial agent also comprises domestication bacteria liquid. The invention also protects application ofthe composite heterotrophic nitrifying bacterial agent in nitrogen removal treatment of waste water containing ammonia and nitrogen, and the application is characterized in that: the composite heterotrophic nitrifying bacterial agent with an inoculation amount of 0.1 to 5% is inoculated into a water body containing ammonia and nitrogen; the number of live bacteria in the composite heterotrophic nitrifying bacterial agent is 109 to 1010 / mL; treating conditions are that temperature is 20 to 35 DEG C, pH is 6 to 9 and aeration time is 48 to 72 h. The composite heterotrophic nitrifying bacterial agent provided in the invention has strong ability in enduring waste water with a high concentration of ammonia and nitrogen, can be used for nitrogen removal treatment of waste water with a concentration of ammonia and nitrogen greater than 400 mg / L, has a high removal capability to waste water with a high concentration of ammonia and nitrogen, with a removal rate of ammonia and nitrogen being more than 99%, substantially reduces the content of ammonia and nitrogen in a water body and improves water quality.

Description

technical field [0001] The invention relates to a compound heterotrophic nitrifying bacteria agent and its application in denitrification treatment of high-concentration ammonia nitrogen wastewater, belonging to the field of environmental microorganisms. Background technique [0002] Ammonia nitrogen is a common pollutant in water bodies. It often contains high concentrations of ammonia nitrogen in aquaculture water, industrial wastewater, agricultural wastewater and landfill leachate. If the ammonia nitrogen in these wastewater is not effectively treated, it will harm the environment and human health. Therefore, the removal of ammonia nitrogen is a key issue in sewage treatment. [0003] There are generally physical, chemical and biological methods for denitrification of sewage. Practice has proved that compared with the first two methods, biology is one of the most economical and effective methods. Its treatment cost is low, the effect is good, and there is no secondary po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/36C02F3/34C12R1/38C12R1/06C02F101/16
Inventor 司文攻
Owner JIANGSU JIANGDA ECOLOGICAL SCI & TECHCO
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