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Method for extracting and separating beta-elemene from Eupatorium adenophorum

A technology of Eupatorium adenophane and elemene, which is applied in the field of extraction and separation of β-elemene, can solve the problems of high cost, high price of β-elemene, high cost of extraction and purification, and achieve the effect of an effective method

Active Publication Date: 2014-05-14
靖江市华泰机械制造有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since the traditional Chinese medicine zedoary, myrrh, citronella, and goldenrod are all traditional Chinese medicines, the cost is relatively high, and the content of β-elemene is not high, so the cost of extraction and purification is relatively high, resulting in the extremely expensive price of β-elemene , seriously hampering the popularization and application of the drug

Method used

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  • Method for extracting and separating beta-elemene from Eupatorium adenophorum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1 Extraction and separation of β-elemene from Eupatorium adenophorum by distillation

[0067] A. Raw material preparation: collect the stems and leaves of Eupatorium adenophorum, dry them naturally in the sun, crush them, and pass through a 40-mesh sieve.

[0068] B. Extraction of volatile oil: Get 100g of the raw material obtained in step A, put it into a distillation bottle, add 500mL of deionized water or distilled water, 200mL of ethylene glycol, 50mL of ethanol, 3-5 zeolites, heat and distill at normal pressure, and collect 80 Distillate at -150°C. Petroleum ether (boiling range 30-60° C.) was added to the obtained distillate at a ratio of 1:1, and the extraction was repeated three times. The petroleum ether layers were separated and combined, and the solvent was vacuum-dried at 60° C. to obtain a volatile oil (purity>40%).

[0069] C, HPLC preparation: the volatile oil obtained in step B is separated with the following HPLC system: Shimadzu HPLC-10A, C18...

Embodiment 2

[0070] Example 2 Extraction and separation of β-elemene from Eupatorium adenophorum by distillation

[0071] A. Raw material preparation: collect the stems and leaves of Eupatorium adenophorum, and beat them into pulp with a pulverizer.

[0072] B. Extraction of volatile oil: Take 100g of the raw material obtained in step A, put it into a distillation bottle, add 500mL of deionized water or distilled water, 200mL of ethylene glycol, 50mL of ethanol, 3-5 zeolites, heat and distill at normal pressure, and collect 80 Distillate at -150°C. Petroleum ether (boiling range 30-60° C.) was added to the obtained distillate at a ratio of 1:1.5, and the extraction was repeated three times. The petroleum ether layers were separated and combined, and the solvent was vacuum-dried at 60° C. to obtain a volatile oil (purity > 35%).

[0073] C, HPLC preparation: the volatile oil obtained in step B is separated with the following HPLC system: Shimadzu HPLC-10A, C18 preparation column (10mm * 1...

Embodiment 3

[0074] Example 3 Extraction and separation of β-elemene from Eupatorium adenophorum by distillation

[0075] A. Raw material preparation: collect the stems and leaves of Eupatorium adenophorum, and beat them into pulp with a pulverizer.

[0076] B. Extraction of volatile oil: Take 100g of the raw material obtained in step A, put it into a distillation bottle, add 500mL of deionized water or distilled water, 200mL of ethylene glycol, 50mL of ethanol, 3-5 zeolites, heat and distill at normal pressure, and collect 80 Distillate at -150°C. In the obtained distillate, cyclohexane was added in a ratio of 1:1, and the extraction was repeated three times. The petroleum ether layers were separated and combined, and the solvent was vacuum-dried at 60° C. to obtain a volatile oil (purity > 37%).

[0077]C. Column chromatography: put the volatile oil obtained in step B on a 300mL HP20 macroporous resin chromatography column, and carry out successively with 600mL of distilled water, 80% ...

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Abstract

The invention belongs to the field of plant extraction, and aims to provide a method for extracting and separating beta-elemene from a noxious weed plant Eupatorium adenophorum. The method provided by the invention comprises the following steps: A. raw material preparation: carrying out pulping, or drying and pulverizing on fresh stems and leaves of Eupatorium adenophorum as a raw material; B. volatile oil extraction: acquiring volatile oil from the raw material; C. column chromatography: carrying out column chromatography on the volatile oil acquired in the step B, collecting an eluate, and removing solvent in the eluate to acquire a crude beta-elemene product; and D. refinement: refining the volatile oil acquired in the step B or the crude product acquired in the step C by a molecular distillation or preparation-type high efficiency liquid chromatography system to acquire the high-purity beta-elemene. The invention has the advantages of high product purity, high yield and low cost, and provides a brand-new, cheap and effective way for the development and treatment of Eupatorium adenophorum and the acquisition of beta-elemene.

Description

technical field [0001] The invention belongs to the field of plant extraction, in particular to a method for extracting and separating β-elemene from the malignant weed plant Eupatorium adenophorum. Background technique [0002] Eupatorium adenophorum (Eupatorium adenophorum Spreng), also known as destroying grass, liberating grass, airplane grass, etc., is a plant of the genus Eupatorium adenophorum in the family Asteraceae. The plant is widely distributed in Yunnan, Guangxi, Sichuan and other places in my country, and is extremely rich in resources. It belongs to tropical and subtropical invasive weed plants, causing great harm to local agricultural and animal husbandry production and a great threat to local ecological security. [0003] β-elemene, Latin name β-Elemenum, English name β-elemene, chemical name (1S, 2S, 4R) 1-methyl-1-vinyl-2,4-diisopropenylcyclohexyl alkyl. The structural formula is as follows: [0004] [0005] β-Elemene is a series of national scienc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C13/19C07C7/00C07C7/04C07C7/12
Inventor 韦会平赵牧阎妍李勇刘文权魏小庆
Owner 靖江市华泰机械制造有限公司
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