Bone morphogenetic protein-2/basic fibroblast growth factor (BMP-2/bFGF) double-gene chitosan nano-microcapsules and application thereof

Abstract

The invention provides bone morphogenetic protein-2 / basic fibroblast growth factor (BMP-2 / bFGF) double-gene chitosan nano-microcapsules. The substrate of the nano-microcapsules is chitosan, the coated plasmids are green fluorescent protein marked BMP-2 and red fluorescent protein marked human bFGF, and the mass ratio of the plasmids to the chitosan is 1:1. Experiments prove that the BMP-2 and bFGF double genes coated by the chitosan nano-microcapsules can enter cells simultaneously and are effectively expressed, so the nano-microcapsules can be applied to the preparation of a medicine for thegene therapy of bone defect. The preparation method for the microcapsules is simple, conditions are mild, the effect is good, the chitosan can be completely degraded and metabolized in vivo, the decomposition product and metabolite of the chitosan are harmless to human health, and a good application prospect is achieved.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to a BMP-2 / bFGF double-gene chitosan nanocapsule and its application. The plasmids wrapped are BMP-2 (bone morphogenetic protein- 2) and bFGF (basic fibroblast growth factor), the BMP-2 and bFGF double genes wrapped in the chitosan nano-capsule can be simultaneously transfected into cells and effectively expressed. Background technique [0002] Bone defects are often caused by diseases such as trauma, infection, and tumors, which can cause serious human dysfunction and appearance deformity, and have a huge impact on patients' work, life, and social interaction. It is a major problem in clinical medicine. At present, the treatment of bone defects mainly includes autologous bone graft repair and filling with various materials. The main disadvantages of the former are donor site damage and limited bone supply; the main disadvantages of the latter are graft rejection and poor oste...

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Problems solved by technology

[0004] However, there are at least the following problems in gene therapy for bone defects at present: ①The research focuses on a single gene, but in fact, in the process of fracture healing and bone formation, multiple genes are involved and co-expressed; ②The gene transfection mostly uses Viruses are used as carriers, generally only one gene is transferred, and there are many problems such as immunogenicity and safety at the same time; ③The source of seed cells is limited, and the acquisition of bone marrow stromal cells is cumbersome and difficult to be accepted by patients

[0008] At present, viruses are mostly used as vectors for gene transfection, such as retroviruses, adenoviruses, etc. Although some success has been achieved, a viral vector can generally only transfer one target gene, and at the same time, there are still many problems in terms of expression time, immunogenic response and safety. there are many problems

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