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Composite micro-ecological preparation as well as premixed material and application of preparation in feed additive

A composite micro-ecology and premix technology, applied in microorganism-based methods, applications, microorganisms, etc., can solve the problems of instability of gastric acid and bile salts, affecting application effects, and intolerance to antibiotics, so as to improve immunity and resistance to antibiotics. disease ability, improve feed utilization, and promote the effect of animal growth

Active Publication Date: 2012-04-11
BEIJING DABEINONG TECH GRP CO LTD +2
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are many types of micro-ecological products on the market, and the quality is uneven. There are mainly the following problems: (1) the content of live bacteria is low, and studies have shown that if the concentration of a kind of bacteria in the cecal content is less than 10 7 cfu / g, the enzymes and metabolites produced by the bacteria are not enough to affect the host, and it is difficult to meet the treatment needs; (2) the water content is too high, which greatly shortens the shelf life of the probiotics, which also affects the quality of the probiotics. (3) Not resistant to antibiotics; (4) Unstable to gastric acid and bile salts, so that only a few strains are still active after entering the intestinal tract, and the number of viable bacteria required to play a role cannot be reached
Due to these quality problems of probiotics, it affects its application effect in animal feeding and limits the wide application of probiotics

Method used

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  • Composite micro-ecological preparation as well as premixed material and application of preparation in feed additive
  • Composite micro-ecological preparation as well as premixed material and application of preparation in feed additive
  • Composite micro-ecological preparation as well as premixed material and application of preparation in feed additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1. Isolation, screening, breeding and identification of excellent strains

[0022] 1) Enrichment culture: Fresh feces and intestinal contents samples of healthy animals (pigs or poultry) are inoculated into BPY liquid culture center, and cultured at 37°C for 48h;

[0023] 2) Separation and purification of Bacillus licheniformis and Bacillus pumilus: Step 1) The enrichment culture is water-bathed at 100°C for 5 minutes, and then streaked culture on a BPY medium plate, and each colony with characteristic morphology is picked from it and streaked Cultivate and separate until it is a pure colony, check with Gram staining microscope, inoculate the isolated suspected pure colony into the slope of the test tube to proliferate, and then store it in the refrigerator at 4℃ for later use;

[0024] Colony morphology: The surface of the colony is rough, dull, milky white and opaque, with irregular edges, with a diameter of 2-3mm. With the extension of the culture time, it becomes r...

Embodiment 2

[0051] Example 2. Determination of stress resistance and biological performance of excellent strains

[0052] 1. The biological performance and stress resistance of Bacillus licheniformis, Bacillus pumilus and Lactobacillus acidophilus:

[0053] 1) Preparation of the culture of Bacillus licheniformis: inoculate the slant strains stored in the refrigerator into the BPY seed medium for activation, culture at 37° C. and 200 rpm for 18 hours to obtain a culture with a spore rate above 95%.

[0054] 2) Preparation of the culture of Bacillus pumilus: inoculate the slant strains stored in the refrigerator into the BPY seed culture medium for activation, and cultivate it at 35°C and 100 rpm for 16 hours to obtain;

[0055] 3) Preparation of Lactobacillus acidophilus culture: inoculate the slant strains stored in the refrigerator into the MRS seed medium for activation, and then culture it at 37°C for 16 hours to obtain;

[0056] 4) Determination of acid resistance: the above-prepared culture wa...

Embodiment 3

[0102] Example 3. Preparation of bacterial powder

[0103] 1. Enterococcus faecalis (CMGCC No.5092)

[0104] (1) High-density fermentation of Enterococcus faecalis (CMGCC No.5092)

[0105] The fermentation medium used to ferment Enterococcus faecalis is composed of the following ingredients: brown sugar 18g / L, peptone 4g / L, beef extract 3g / L, yeast extract, 0.8 / L, magnesium sulfate heptahydrate 0.4g / L, manganese sulfate 0.005g / L, pH is 7.2.

[0106] The fermentation conditions are as follows: fermentation temperature is 37°C, fermentation time is 12h, pH value is 7.2, rotation speed is 100rpm, and initial inoculation amount is 2%. After fermentation, 7.3×10 9 cfu / ml bacterial liquid.

[0107] (2) Preparation of Enterococcus faecalis (CMGCC No.5092) powder

[0108] The high-temperature spray drying of Enterococcus faecalis is carried out, and the mass-volume ratio of the protective agent to the fermentation broth is 1:3 to prepare the protective agent. The protective agent components ar...

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Abstract

The invention belongs to the technical field of feed micro-ecology and relates to a composite micro-ecological preparation as well as a premixed material and an application of the preparation in a feed additive. The preparation contains Lactobacillus acidophilus powder and any two, three or four bacterium powders selected from Bacillus licheniformis, Bcillus pumilus, enteroccus and Bcillus subtilis; the preparation has high live bacteria content and low moisture content, has stress tolerance properties such as gastric acid resistance, cholate resistance, high temperature resistance, common antibiotics tolerance and the like and has the probiotic functions of producing acid and enzyme, inhibiting pathogenic bacteria and the like. By using the composite micro-ecological preparation of the invention, the utilization rate of the feed can be improved, the yields of meat, eggs, milk and the like are increased, the growth of animals is promoted, the immunity and disease-resistant capability of an animal organism are improved, and partial preparation can be used for replacing antibiotics, thereby changing the quality of an animal product.

Description

Technical field [0001] The invention belongs to the technical field of feed microecological, and relates to a composite microecological preparation and its application in feed additives and a premix. Background technique [0002] At present, antibiotics are basically still used for the prevention and treatment of animal diseases. However, due to the long-term and large-scale use of antibiotics, pathogenic bacteria have increased drug resistance, flora imbalance, decreased immunity, high disease recurrence rates, and drug residues. Seriously, it also seriously endangers the health of animals and humans. Under this situation, it is an inevitable trend to change the concept of breeding, prevention is more important than treatment, and to develop and apply new green feed additives that are non-toxic and drug-free. [0003] A green and safe feed additive that is commonly used and studied in the microecological preparation room. This type of preparation is produced by industrialized hig...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04A23K1/16C12R1/23C12R1/10C12R1/07C12R1/125C12R1/01
Inventor 杜建涛付维来刘鹏彭子欣王安如
Owner BEIJING DABEINONG TECH GRP CO LTD
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