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Method for degrading zearalenone

A technology of zearalenone and seeds, applied in application, food preparation, food science, etc., to achieve the effect of restoring application value

Active Publication Date: 2013-03-13
QINGDAO VLAND BIOTECH GRP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the degradation of zearalenone by the fermentation product of Aspergillus niger

Method used

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  • Method for degrading zearalenone

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: the preparation of aspergillus niger fermentation product

[0018] 1. Incline cultivation

[0019] Aspergillus niger species CGMCC 3.316 was inserted into a test tube containing PDA medium (20% potato, 2% glucose, 2% agar), and cultured statically at 30° C. for 3-5 days.

[0020] 2. Preparation of spore suspension

[0021] Wash the Aspergillus niger spores on the slant medium with sterile water and dilute to about 1×10 9 cells / ml to make a spore suspension.

[0022] 3. Preparation of primary seed solution:

[0023] Add 100ml PDY medium (20% potato, 2% glucose, 1% yeast extract) into a 500ml shake flask, insert the spore suspension according to the inoculum size of 8%, cultivate for 2 days at 25°C, 120-150r / min.

[0024] 4. Solid fermentation

[0025] Weigh 50% of rice straw powder in proportion, (NH 4 ) 2 SO 4 8%, bran 42%, then add water about 4.9 times the volume of solid matter, mix thoroughly, and sterilize to obtain a solid fermentation mediu...

Embodiment 2

[0029] Embodiment 2: Aspergillus niger fermentation product is to the degradation of ZEN standard substance

[0030] 1. The effect of the addition amount of Aspergillus niger fermentation product on the degradation rate of ZEN standard

[0031] According to the residence time of chyme in the gastrointestinal tract of animals, the degradation rate of the added amount of Aspergillus niger fermentation product to the ZEN standard was measured in the simulated animal stomach and intestinal environment, and the optimal amount was determined.

[0032] 1.1 Influence of the addition amount of Aspergillus niger fermentation product on ZEN degradation rate in simulated gastric juice

[0033] Add 24ml of sterilized pH 2.0 PBS buffer solution, 0.25ml of pepsin solution, 0.25ml of ZEN standard substance with a concentration of 50ppm in the Erlenmeyer flask, add 0.05%, 0.10%, 0.15%, 0.2%, 0.3%, respectively. 0.4% (mass ratio) dry powder of Aspergillus niger fermentation broth, two replicat...

Embodiment 3

[0055] Example 3 Degradation of Aspergillus niger fermentation product to ZEN in moldy corn

[0056] 1. Determination of the total amount of ZEN in moldy corn

[0057] Moldy corn was crushed and passed through a 20-mesh sieve. According to the assay method required by the British NEOGEN company Zearalenone Veratox (ZEN high-sensitivity detection kit), the ZEN content was determined to be: 1536.78ppb

[0058] 2. Buffer preparation

[0059] PBS (pH6.5): KCl: 0.2g KH 2 PO 4 : 0.204g Na 2 HPO 4 2H 2 O: 1.442gNaCl: 8.066g dissolved in 1000ml distilled water, adjust the pH value to 6.5

[0060] PBS (pH2.0): KCl: 0.2g KH 2 PO 4 : 0.204g Na 2 HPO4·2H 2 O: 1.442gNaCl: 8.066g dissolved in 1000ml distilled water, adjust the pH value to 2.0

[0061] Pepsin solution: Accurately weigh 1.333 g of pepsin (1:10000), dissolve it in 10 ml of PBS solution with pH 2.0, stir well to dissolve it completely.

[0062] Trypsin solution: Accurately weigh 1.0 g of trypsin (1:250), dissolve it...

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Abstract

The invention relates to a method for degrading zearalenone, which is characterized in that: a fermentation product of aspergillus niger is used for degrading zearalenone in musty grain, wherein the fermentation product of the aspergillus niger is the dry powder of fermentation liquor. In the invention, because the fermentation product of aspergillus niger is used for degrading zearalenone, zearalenone in the musty grain can be effectively degraded, thereby restoring the application value of the musty grain. Meanwhile, by measuring the effect of the fermentation product of aspergillus niger on degrading ZEN (zearalenone) in the in-vitro environment of simulating the gastrointestinal tract, optimal degrading conditions of the fermentation product of aspergillus niger on ZEN are obtained, thereby providing a solid foundation for further developing the fermentation product of aspergillus niger into a biological antidote for degrading ZEN in the grain and feed.

Description

technical field [0001] The invention belongs to the technical field of biological detoxification, and in particular relates to a method for degrading zearalenone, that is, using a fermentation product of Aspergillus niger to degrade zearalenone. Background technique [0002] Zearalenone (Zearalenone, ZEN for short) is a toxic substance produced by the metabolism of toxin-producing fungi of the genus Fusarium, such as F. graminearum and F. tricinctum , Widely present in moldy corn, sorghum, wheat, oats, barley and other cereal crops and dairy products. Zearalenone has an estrogen effect, mainly acting on the reproductive system, and can cause hyperestrogenism in livestock, poultry and experimental mice. Eating foods containing zearalenone in pregnant animals (including humans) can cause Hazards such as miscarriage, stillbirth and teratogenicity. The grains (such as corn and wheat) used in feed and food production are the best substrates for the production of zearalenone. I...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/015A23L1/10A23L5/20A23L7/10
Inventor 马向东徐雪梅李慧芳李佩佩
Owner QINGDAO VLAND BIOTECH GRP
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