Phenol-degrading fungi and application thereof

A technology of phenol degradation and fungi, applied in the field of environmental microorganisms, to achieve strong environmental tolerance and the effect of removing phenol pollution

Inactive Publication Date: 2012-04-04
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the current research focuses on bacteria, but there are few reports on the biodegradation of phenol by fungi. Fungi that have been found to degrade phenol include Fusarium floccieferum (Anselmo et al. Water Sci Technol 1992, 25:161–168) , Aspergillus fumigatus (Jones et al. Arch Microbiol 1995,163:176–181), Graphium sp. (Santos et al. J Basic Microbiol.2003,43:238–248) , Trichosporium dermatoides trichosporon cutaneum have (Gaal A et al. Arch Microbiol.1981, 130:54–58 and Godjevargova T et al. Process Biochem .2003,38:915–20), Penicillium sp. (Leitāo. Int J Environ Res Public Health .2009,6:1393–1417. Sumaya Ferreira Guedes et al. Biodegradation.2011,22:409–419), Paecilomyces variotii (Wang et al. J Hazard Mater. 2010,183:366–371 ), tropical yeast Candida tropicalis (Jiang Y et al. Biochem Eng J. 2005,24:243–247), filamentous fungi (Santos V L&Linardi V R.Process Biochem.2004,39:1001-1006), white Sapro fungus white rot fungi (Sanin S. et al. Bull Environ. Contam Toxicol. 2005,75:466–473), these fungi have been shown to have the potential to degrade phenol

Method used

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  • Phenol-degrading fungi and application thereof
  • Phenol-degrading fungi and application thereof
  • Phenol-degrading fungi and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Screening and identification of phenol-degrading fungal strains

[0028] Inorganic culture medium (mg / L): NaNO 3 2.000 g, K 2 HPO 4 1.000 g, KCl 0.500 g, MgSO 4 .7H 2 O 0.500 g, FeSO 4 .7H 2 O 0.010 g;

[0029] Screening plate medium (mg / L): NaNO 3 2.000 g, K 2 HPO4 1.000 g, KCl 0.500 g, MgSO 4 .7H 2 O 0.500 g, FeSO 4 .7H 2 O 0.010 g, phenol 0.200 g, agar 18.000 g, H 2 O1000mL;

[0030] Storage medium (mg / L): 200.0 g potatoes, 20.0 g sucrose, 0.100 g phenol, 18.00 g agar, H 2 O 1000 mL;

[0031] PDA culture medium (mg / L): potato 200.0 g, sucrose 20.0 g, agar 18.00 g, H 2 O 1000 mL.

[0032] Take seawater samples from the East China Sea near Ningbo (longitude 125-128°, latitude 32-35°), first sterilize the 0.22 μm microporous membrane filter, and then take the seawater sample liquid and filter it through a sterile microporous membrane. The microorganisms in the sample liquid are trapped on the filter membrane. Take out the filter membrane, cut it...

Embodiment 2

[0046] Biodegradation of Phenol by Strain PHDE-4

[0047] Strain PHDE-4 was inoculated into 100 mL of sterile screening culture medium containing 100 mg / L phenol, and cultured at 28°C with shaking at 150 rpm for 2 days until the logarithmic phase of growth was used as the seed solution.

[0048] Take 8×1 mL of the above seed solution and inoculate them into 100 mL of sterile phenol containing 200 mg / L, 400 mg / L, 600 mg / L, 800 mg / L, 1000 mg / L, 1200 mg / L, 1400 mg / L, respectively. L, 1600 mg / L simulated wastewater, at 28 ° C, 150 rpm shaking culture, sampling every day, using 4-aminoantipyridine as color reagent, 510 nm colorimetric method to quantitatively determine the concentration of residual phenol, the result indicated in the attached Figure 8 middle.

[0049] When the phenol content in the simulated wastewater is less than 800 mg / L, phenol can be completely degraded after 10 days; and when the phenol content is 1000 mg / L and 1200 mg / L, the degradation rate of phenol r...

Embodiment 3

[0051] Biodegradation of Phenol by Strain PHDE-4 at Different pH

[0052] Strain PHDE-4 was inoculated into 100 mL of sterile screening culture medium containing 100 mg / L phenol, and cultured at 28°C with shaking at 150 rpm for 2 days until the logarithmic phase of growth was used as the seed solution.

[0053] Take 6 × 1 mL of the above seed solution and inoculate them into 100 mL of sterile simulated wastewater containing phenol (1000 mg / L) and pH 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, respectively, at 28 °C, 150 Shaking at rpm and culturing for 10 days, using 4-aminoantipyridine as a chromogenic reagent, the concentration of residual phenol was quantitatively determined by 510 nm colorimetry. Figure 9 middle.

[0054] Strain PHDE-4 can biodegrade phenol in a wide pH range, but the degradation rate is affected by pH. When the pH is 5.0-7.0, the degradation rate of phenol by strain PHDE-4 can reach the maximum.

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Abstract

The invention relates to a phenol-degrading fungi and application thereof. The fungi bacterial strain is an Aspergillus flavus fungi strain PHDE-4 and is collected in China General Microbiological Culture Collection Center on August 31st, 2011, wherein the collection number is CGMCC No.5189. The phenol-degrading fungi bacterial strain PHDE-4 provided by the invention has the advantages of stronger environmental tolerance, capability of taking the phenol as the only carbon source and energy source to grow and propagate under wider temperature and pH value and more demanding environmental conditions, and capability of better removing the phenol pollution in the environment.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a strain of phenol-degrading fungus, which can biodegrade phenol under the conditions of pH 5.0-8.0 and temperature 10°C-40°C. Background technique [0002] Phenol is widely used as a preservative in the paint, leather and textile industries, as well as in the production of phenolic resins, disinfectants, pharmaceuticals, caprolactam and bisphenol A (Rana Kidak et al. Ultrasonics Sonochemistry.2006,13: 195-199). The presence of phenol in the aquatic environment can cause significant harm to aquatic organisms, and low concentrations of 5 to 25 mgL-1 of phenol are lethal to fish (P.R. Gogate, et al. Environ.Res. 8 (2004) 501 –551.); Kumar et al. reported that phenol is carcinogenic to humans, so wastewater containing phenol must be treated before it can be discharged. Compared with physical and chemical treatment methods, biological treatment of phenol has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A62D3/02C12R1/67A62D101/28
Inventor 程波户业丽
Owner WUHAN INSTITUTE OF TECHNOLOGY
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