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Method for preparing bitter almond extract product

A kind of bitter almond and extract technology, which is applied in the field of preparing bitter almond extract, can solve the problems of denaturation of bitter almond protein, influence the deep development and utilization of bitter almond protein, etc., and achieve the effect of preventing excessive denaturation

Inactive Publication Date: 2013-01-23
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, high temperature treatment led to severe denaturation of amygdalin, which affected the in-depth development and utilization of amygdalin

Method used

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  • Method for preparing bitter almond extract product
  • Method for preparing bitter almond extract product
  • Method for preparing bitter almond extract product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1) 100 g of bitter almonds are dried in the sun, so that the water activity of the bitter almonds is 0.70, and the dried bitter almonds are obtained;

[0031] According to the Soxhlet extraction method, the bitter almond oil in the bitter almond as the raw material is determined, and the amygdalin in the bitter almond as the raw material is determined by high performance liquid chromatography, and the amygdalin as the raw material is determined according to the Kjeldahl method. The protein in the bitter almond is measured, as can be known, wherein the mass percentage composition of oil, amygdalin and protein is respectively 45.8%, 3.25% and 22.6%;

[0032] 2) Under the condition that the water activity of the dried bitter almonds obtained in step 1) is kept at 0.70, the dried bitter almonds are pulverized to 10 meshes to obtain 96.8 g of pulverized bitter almonds;

[0033] 3) Under the condition that the water activity of the pulverized bitter almonds obtained in step 2...

Embodiment 2

[0044] 1) 100 g of bitter almonds are dehydrated with 20 mL of dehydrating agent ethanol, so that the water activity of bitter almonds is 0.53, and dried bitter almonds are obtained;

[0045]According to the Soxhlet extraction method, the bitter almond oil in the bitter almond as the raw material is determined, and the amygdalin in the bitter almond as the raw material is determined by high performance liquid chromatography, and the amygdalin as the raw material is determined according to the Kjeldahl method. The protein in the bitter almond is measured, as can be known, wherein the mass percentage composition of oil, amygdalin and protein is respectively 45.8%, 3.25% and 22.6%;

[0046] 2) Under the condition that the water activity of the dried bitter almonds obtained in step 1) is kept at 0.53, the dried bitter almonds are pulverized to 120 mesh to obtain 94.52 g of pulverized bitter almonds;

[0047] 3) under the condition that the water activity of the bitter almonds afte...

Embodiment 3

[0058] 1) Dry 100 g of bitter almonds by freeze-drying, so that the water activity of bitter almonds is 0.40, and obtain dried bitter almonds; in this freeze-drying step, first pre-freeze the material to -40°C, and then turn on the vacuum pump , so that the vacuum degree is 20-40Pa to carry out the vacuum freeze-drying operation, and the freeze-drying is completed when the material temperature is close to room temperature.

[0059] According to the Soxhlet extraction method, the bitter almond oil in the bitter almond as the raw material is determined, and the amygdalin in the bitter almond as the raw material is determined by high performance liquid chromatography, and the amygdalin as the raw material is determined according to the Kjeldahl method. The protein in the bitter almond is measured, as can be known, wherein the mass percentage composition of oil, amygdalin and protein is respectively 45.8%, 3.25% and 22.6%;

[0060] 2) Under the condition that the water activity of...

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Abstract

The invention discloses a method for preparing a bitter almond extract product. The method provided by the invention comprises the following steps of: 1) drying bitter almond until the water activity of bitter almond reaches 0.10-0.70; 2) crushing to 10-120 meshes; 3) extracting bitter almond oil on the basis of maintaining the water activity unchanged; 4) separating and extracting bitter almond meal by using an ethanol aqueous solution at the concentration of 50-95% to obtain amygdalin and bitter almond proteins. When crushing bitter almond and extracting bitter almond oil by the adoption ofthe method, amygdalase activity is reduced by controlling the water activity; and during the separation and extraction of the amygdalin and bitter almond proteins, the ethanol concentration and the operation temperature are controlled to reduce the amygdalase activity and prevent the bitter almond protein from overdenaturation. Therefore, the method provided by the invention is used to realize the full utilization of bitter almond oil, bitter almond protein and amygdalin and lay a foundation for the refined and deep processing and comprehensive utilization of bitter almond.

Description

technical field [0001] The invention relates to a method for preparing bitter almond extract. Background technique [0002] Bitter almond is a commonly used traditional Chinese medicine, which was first recorded in "Shen Nong's Materia Medica". It is the dried apricot (Prunus armeniaca), mountain apricot (P. mature seeds. Bitter almond resources are very rich in my country, mainly produced in Northeast China, North China, Northwest China, Inner Mongolia, Xinjiang, Liaoning, Jilin and the Yangtze River Basin (Cheng Lihui. Discussion on the historical evolution of bitter almond processing [J]. Strait Pharmacy, 2009: 21 (4) :84-86). [0003] The main components of bitter almonds include amygdalin (3%-4%), bitter almond oil (35%-50%) and amygdalin (24%-30%). Laetrile has the effects of antitussive and asthmatic, antitumor, hypoglycemic and anticoagulant effects, so far it has become a commonly used expectorant and antitussive agent and auxiliary anticancer drug in medicine. In...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H15/18C07H1/08C07K1/14C11B1/00C11B1/04C11B1/06C11B1/10
CPCY02P20/54
Inventor 田金强兰彦平周连第王波周家华常虹
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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