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Extraction method of morindea officinalis polysaccharide

An extraction method and Morinda officinalis technology, applied in the extraction of Morinda officinalis polysaccharide, the field of extracts of Chinese medicinal materials, can solve the problems of low yield and purity of Morinda officinalis polysaccharide, complex process steps, poor deproteinization effect, etc. To achieve the effect of good medicinal effect, simple process and short production cycle

Inactive Publication Date: 2012-02-15
朱孟勇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method can extract Morinda officinalis polysaccharides, the process steps of this method are relatively complicated, and the extracted Morinda officinalis polysaccharides contain more impurities, and the effect of removing protein is poor, and the yield and purity of Morinda officinalis polysaccharides obtained Both are low, which is not conducive to practical production application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Select the rhizomes of Morinda officinalis, wash them first, remove impurities, dry them at 60°C, and then crush them to 60 mesh with a pulverizer to obtain Morinda officinalis powder. Weigh 100g of Morinda officinalis powder without adding it to 400g of chloroform / methanol mixed solvent (chloroform: the volume ratio of methanol is 2: 1), be warming up to under the condition of 60 ℃, reflux 2 hours, carry out degreasing, decolorization treatment, after reflux finishes, filter, remove filtrate, collect filter cake, will The collected filter cake was added into an aqueous ethanol solution with a volume fraction of 80%, and refluxed for 2 hours under the condition of raising the temperature to 80°C. After the reflux was completed, filter, remove the filtrate, and then add the obtained solid to 100mL of distilled water, and heat up to Stir and extract at 60°C for 1 hour, filter, collect the filtrate, and extract the remaining residue; raise the temperature of the collected ...

Embodiment 2

[0032] Select the rhizomes of Morinda officinalis, wash them first, remove impurities, dry them at 60°C, and then crush them to 40 mesh with a pulverizer to obtain Morinda officinalis powder. Weigh 100g of Morinda officinalis powder without adding it to 1500g of chloroform / methanol mixed solvent (chloroform: the volume ratio of methanol is 4: 1), be warming up to under the condition of 60 ℃, reflux 2 hours, carry out degreasing, decolorization treatment, after reflux finishes, filter, remove filtrate, collect filter cake, will The collected filter cake was added into an aqueous ethanol solution with a volume fraction of 85%, and refluxed for 2 hours under the condition of raising the temperature to 80°C. After the reflux was completed, filter, remove the filtrate, and then add the obtained solid to 100mL of distilled water, and heat up to 50°C, stir and extract for 2 hours, filter, collect the filtrate, add the remaining residue to water, heat up to 50°C, stir, extract for 2 h...

Embodiment 3

[0035] Select the rhizomes of Morinda officinalis, wash them first, remove impurities, dry them at 60°C, and then crush them to 80 mesh with a pulverizer to obtain Morinda officinalis powder. Weigh 100g of Morinda officinalis powder without adding it to 400g of chloroform / methanol mixed solvent (chloroform: the volume ratio of methanol is 3: 1), be warming up to under the condition of 60 ℃, reflux 2 hours, carry out degreasing, decolorization treatment, after reflux finishes, filter, remove filtrate, collect filter cake, will The collected filter cake was added into ethanol aqueous solution with a volume fraction of 75%, and refluxed for 2 hours under the condition of raising the temperature to 80° C. After the reflux was completed, filtered, and the filtrate was removed, and the obtained solid was added to 100 mL of distilled water, and the temperature was raised to 70°C, stir and extract for 0.5 hours, filter, collect the filtrate, add the remaining residue to water, heat up...

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Abstract

The invention provides an extraction method of morindea officinalis polysaccharide, belonging to the technical field of traditional Chinese medicines and solving the problems that in the existing morindea officinalis polysaccharide extraction method, the extraction method has complicated process steps and a poor protein removal effect, and the yield and purity of the obtained morindea officinalispolysaccharide are low. The extraction method of morindea officinalis polysaccharide in the invention comprises the following steps of: drying morindea officinalis rhizome and then smashing to 40-80 meshes; adding a chloroform / methanol mixed solvent in morindea officinalis powder for carrying out reflux extraction; carrying out alcohol extraction on a filter cake obtained by filtration and then adding water for lixiviation; subsequently, filtering and concentrating a filtrate, carrying out alcohol precipitation on a concentrated liquid and then filtering so as to obtain the crude morindea officinalis polysaccharide; adding the crude morindea officinalis polysaccharide into a n-butanol / trichloroacetic acid mixed solvent for processing, and entering a chromatographic column for elution; andcarrying out concentration, alcohol precipitation, filtration and drying on an effluent liquid so as to obtain the morindea officinalis polysaccharide. The extraction method of the morindea officinalis polysaccharide in the invention has a simple process and a good protein removal effect, and the extracted morindea officinalis polysaccharide has less impurities and high yield and purity.

Description

technical field [0001] The invention relates to an extract of traditional Chinese medicinal materials, and more specifically relates to a method for extracting Morinda officinalis polysaccharides, which belongs to the technical field of traditional Chinese medicines. Background technique [0002] Morinda officinalis is a dicotyledonous plant Rubiaceae, mainly distributed in tropical and subtropical regions of Guangdong, Guangxi, Fujian, Hainan and other regions. It is a traditional Chinese medicinal material with various pharmacological activities and high medicinal value. , is hailed as one of the famous "Four Great Southern Medicines" in China. As a traditional Chinese medicine, Morinda officinalis has many functions such as increasing the immune function of the body, improving hematopoietic function, anti-aging, anti-depression, anti-tumor, regulating the nervous system and endocrine system, etc. researcher. [0003] Morinda officinalis Chinese medicinal materials conta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 朱孟勇王彩娇
Owner 朱孟勇
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