Broad-spectrum antiviral medicament as well as preparation method and application thereof

Abstract

The invention discloses a broad-spectrum antiviral medicament as well as a preparation method and application thereof and relates to a creative medicament in the technical field of biology. The broad-spectrum antiviral medicament consists of a protein transduction label, a double-stranded RNA (Ribonucleic Acid) detection domain and an apoptosis induction domain. The preparation method comprises the steps of construction, expression and purification of a gene recombinant protein. The application comprises a cell model experiment, an animal model experiment and antiviral action for DNA (Deoxyribonucleic Acid) or RNA virus of dsRNA generated during infection cell. The broad-spectrum antiviral medicament is designed according to a pure cell antiviral natural mechanism and has the advantages of high reliability, broad-spectrum virus resistance, high specificity, no toxic or side effect, high safety, favorable repeatability of experiment result, easiness in popularization, simpleness and convenience in research and production and low cost.

Description

technical field [0001] The invention relates to innovative drugs in the field of biotechnology, in particular to a broad-spectrum antiviral drug and its preparation method and application. Background technique [0002] Nowadays, viral pathogens that cause human viral diseases are attracting more and more attention, including emerging viruses in nature such as avian influenza virus, SARS (coronavirus), etc., clinical viruses such as hepatitis virus, HIV (AIDS virus), etc. , and related potential biological panic viruses such as smallpox, Ebola virus, etc. Unfortunately, for the above-mentioned viruses, there is still a lack of very effective preventive and therapeutic measures. The main measures can be divided into three categories: [0003] 1. Interferon and anti-inflammatory factors: Although they are effective for some viruses, they lack specificity for other viruses. Since they are cytokines, if they are excessively ingested, they will interact with factors such as the i...

AI Technical Summary

Problems solved by technology

[0003] 1. Interferon and anti-inflammatory factors: Although they are effective for some viruses, they lack specificity for other viruses. Since they are cytokines, if they are excessively ingested, they will interact with factors such as the immune system and cause host cell-type Unbalanced factor levels, resulting in greater side effects

[0004] 2. Specific inhibitors: specific inhibitors (such as HIV protease inhibitors) that inhibit the necessary targets in the process of virus replication and transcription, but its disadvantage is that when the virus mutates and changes the corresponding targets, it can cause It effectively escapes the inhibitory effect of inhibitors, so inhibitors cannot quickly and effectively respond to emerging viral pathogens, and the side effects of drugs after use cannot be predicted

[0005] 3. Vaccines: Due to the long cycle of developing specific vaccines for different viruses, the prevention and treatment of emerging viral pathogens are not timely and effective, and the development of vaccines is also quite difficult, which is mainly reflected in: 1. Viruses There are many types; 2. The various subtypes of the same virus are complex. For example, the vaccine can only specifically target one virus, and has no cross-protective response to other types of viruses, and the host cannot be well protected.

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