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Environment biological disinfectant with phages as main components and application thereof

A technology of biological disinfectant and main components, applied in the field of bioengineering, to achieve the effect of strong pertinence, good disinfection and purification effect, and improved killing effect

Inactive Publication Date: 2012-02-15
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, in preparations that have killing and purifying effects on pathogenic bacteria such as Escherichia coli, Salmonella, and Shigella, the above-mentioned Escherichia coli phage, Salmonella enteritidis phage, Salmonella pullorum phage, and Shigella flexneri phage are used for Composite composition, not reported

Method used

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  • Environment biological disinfectant with phages as main components and application thereof
  • Environment biological disinfectant with phages as main components and application thereof
  • Environment biological disinfectant with phages as main components and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1, the composition of environmental biological disinfectant

[0017] The 4 kinds of purified phages preserved in our laboratory were respectively activated and then diluted to make the concentrations ≥10 4 Pfu / ml is enough, then mix according to the following volume ratio, mix evenly, then add Pulsatilla chinensis extract and cinnamon extract according to the following volume ratio, mix thoroughly, and then add an appropriate amount of adjuvants. The formula consists of the following:

[0018] Salmonella enteritidis phage (CTCC M 2010226): 10-30%;

[0019] Salmonella pullorum phage (CCTCC M 2011042): 10-30%;

[0020] Coliphage (CCTCC M 209208): 10-30%;

[0021] Shigella flexneri phage (CCTCC M 209029): 10-30%;

[0022] Cinnamon extract (purchased) 5-8%;

[0023] Pulsatilla extract (self-made) 5-8%;

[0024] Adjuvant supplemented to 100%. Prepared into a liquid and set aside;

[0025] Wherein: the Pulsatilla extract is obtained in this way.

[0026] T...

Embodiment 2

[0028] Embodiment 2: The effect of environmental disinfectant on the sterilization and purification of room air natural bacteria

[0029] This experiment selects a kind of commercialized disinfectant: peracetic acid disinfectant as a comparison, and studies the evolutionary disinfection ability of the disinfectant of the present invention to the natural bacteria in the room air.

[0030] The environmental biological disinfectant of the present invention is made into a solution, and aerosol is generated through a sprayer, and the doors and windows are sealed for 1 hour before and after spraying. It is also sealed for 1 hour before and after peracetic acid spraying. The above two different disinfectants were all used aerosol spray method, and samples were taken 30 minutes before and 30 minutes after disinfection for bacterial culture.

[0031] Air disinfection monitoring method: According to the requirements of the "Disinfection Technical Specifications" issued by the Ministry ...

Embodiment 3

[0036] Embodiment 3: Disinfection effect experiment of disinfectant in chicken coop

[0037] In this experiment, three laying hen houses were selected for the experiment.

[0038] Disinfection method: Chicken house 1: Thoroughly disinfect with a sprayer, including the ground and walls, and try to spread the liquid as far as possible. The air, walls and ground in the environment are sampled for bacterial isolation and determination. Inside the chicken house 2: Wipe and disinfect cage frames, egg baskets and feed trolleys with gauze soaked in disinfectant. Chicken coop 3: spray disinfection with peracetic acid, collect air samples in the environment for bacterial isolation and determination.

[0039] Disinfection effect detection: 30 minutes before spray disinfection or wipe disinfection, 30 minutes and 24 hours after disinfection, the air in chicken house 1 and chicken house 3 were sampled to analyze the change of bacteria, and the walls and ground in chicken house 1 were sam...

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Abstract

The invention provides an environment biological disinfectant with phages as main components and application thereof. The biological disinfectant is composed of Escherichia coli phage CCTCC (China Center for Type Culture Collection) M209208, Bacterium enteritidis phage CCTCC M2010226, Salmonella pullorum phage CCTCC M2011042, Shigella flexneri phage CCTCC M209029, cinnamon extract, Chinese pulsatilla extract and auxiliary agents, wherein the purified phages are prepared into liquid with the concentration of more than or equal to 10<4> pfu (plaque forming unit) / mL, and are used for sterilizing and cleansing environment places and devices. The biological disinfectant has the characteristics of strong sterilization capability, rapid sterilization, no toxicity or harm, no irritation, no corrosion, no colorizing performance and the like, is especially suitable for disinfection and cleansing of breeding farms, slaughter houses, livestock product processing factories, food warehouses and other environments and devices, and has better popularization and use values.

Description

technical field [0001] The invention provides an environmental disinfectant with multiple isolated lytic bacteriophages as the main component and its use in the environment of farms, slaughterhouses, food processing plants, hospitals and other places and related utensils such as E. coli, Salmonella, and The application of killing and purifying pathogenic bacteria such as Shigella. Belongs to the field of bioengineering. Background technique [0002] At present, the spread of bacterial diseases in farms shows a trend of multiple, high and mixed infections, which has caused great harm to the control of farm diseases, the benefits of animal husbandry and the pollution of pathogenic bacteria in livestock products. , chicken farms, cattle farms and duck farms, etc.) The investigation on the occurrence and epidemic characteristics of epidemic diseases found that: the bacterial diseases in farms are very severe, and they are important factors that directly affect animal production...

Claims

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Application Information

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IPC IPC(8): A01N65/32A01N65/24A01N63/00A01P1/00A61L2/18
Inventor 王冉张辉包红朵
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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