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Preparation method of halophile photosensitive protein-titanium dioxide nanotube composite

A technology of titanium dioxide and photosensitive protein, applied in biochemical equipment and methods, nanotechnology, nanotechnology, etc., can solve the problem of high probability of photogenerated electron-hole recombination, hindering the industrialization and application of TiO2 photocatalytic materials, and the utilization rate of sunlight low level problem

Inactive Publication Date: 2014-04-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the recombination probability of photogenerated electron-hole pairs in TiO2 photocatalytic materials is high; on the other hand, the utilization rate of sunlight for anatase TiO2 is low, which seriously hinders the industrialization and application of TiO2 photocatalytic materials.

Method used

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  • Preparation method of halophile photosensitive protein-titanium dioxide nanotube composite

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Embodiment 1

[0017] Escherichia coli Rossetta strain containing PET28aJ56 plasmid was inoculated in sterilized LB solid medium containing kana antibiotics by streaking method. After 24 hours, a single clone was picked, inoculated in 5 ml of LB liquid culture medium containing kana antibiotics, 200 rpm, and cultured at 37° C. for 24 hours for activation. Transfer 1ml of activated Escherichia coli into 250ml of LB liquid medium for expanded culture. Transfer the expanded and cultivated Escherichia coli to 2L LB liquid medium according to the inoculum amount of 20ml, and cultivate it at 37°C and 200rpm for 2-3 hours. When it reaches 0.6, add IPTG to the final concentration of 1mM in the bacterial solution, add 0.5% retinal, and induce for 4 hours at 37°C. Centrifuge the bacterium solution that has been induced to express the rhodopsin protein at 4000 rpm for 20 minutes, and collect the bacterium. Add 0.01% β-mercaptoethanol and 10% sarkosyl to the pellet, resuspend the bacteria, and ultraso...

Embodiment 2

[0021] Escherichia coli Rossetta strain containing PET28aJ56 plasmid was inoculated in sterilized LB solid medium containing kana antibiotics by streaking method. After 24 hours, a single clone was picked, inoculated in 10 ml of LB liquid culture medium containing kana antibiotics, 200 rpm, and cultured at 37° C. for 24 hours for activation. Transfer 2ml of activated Escherichia coli into 250ml of LB liquid medium for expanded culture. Transfer the expanded and cultivated Escherichia coli to 2L LB liquid medium according to the inoculation amount of 10ml, and cultivate it at 37°C and 200rpm for 2-3 hours, during which the absorbance OD value of the bacteria solution at 600nm is often measured until the OD When it reaches 0.5, add IPTG to the final concentration of 1mM, add 0.5% retinal, and induce for 4 hours at 37°C. Centrifuge the bacterium solution that has been induced to express the rhodopsin protein at 4000 rpm for 20 minutes, and collect the bacterium. Add 0.01% β-mer...

Embodiment 3

[0025] Escherichia coli Rossetta strain containing PET28aJ56 plasmid was inoculated in sterilized LB solid medium containing kana antibiotics by streaking method. After 24 hours, a single clone was picked, inoculated in 5 ml of LB liquid culture medium containing kana antibiotics, 200 rpm, and cultured at 37° C. for 24 hours for activation. Transfer 5ml of activated and cultured Escherichia coli to 2L of LB liquid medium, and cultivate it at 37°C and 200rpm for 6-8 hours. Add IPTG to the bacterial solution to a final concentration of 1 mM, add 0.5% retinal, and induce for 4 hours at 37°C. Centrifuge the bacterium solution that has been induced to express the rhodopsin protein at 4000 rpm for 20 minutes, and collect the bacterium. Add 0.01% β-mercaptoethanol and 10% sarkosyl to the pellet, resuspend the bacteria, and ultrasonically break at 200W for about 30 minutes. Centrifuge at 12000rpm for 20min, and recover the supernatant. Add 0.5% TritonX-100 to the supernatant, mix w...

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Abstract

The invention provides a halophile photosensitive protein-titanium dioxide nanotube composite and a preparation method thereof. Photosensitive protein is halophilic archaea rhodopsin protein, and the colibacillus is modified to express the rhodopsin protein in a large quantity. After bacteria are broken ultrasonically, Beta-mercaptoethanol and acyl sarcosine are used for extracting membrane protein, and the membrane protein is then processed by TritonX-100. The preparation method for the composite includes the following steps: titanium dioxide nano material is put into protein solution, vacuumized for a period of time and then dehydrated under low temperature, and thereby the halophile photosensitive protein-titanium dioxide nanotube composite is prepared. The process flow related to the invention is simple, used reagents are cheap, and the composite has good visible light-responding ability, can effectively reduce the recombination probability of photoinduced electron-hole pairs and increase the photoelectric response of a TiO2 nanotube array, and has a broad application prospect in the field of photoelectric devices.

Description

technical field [0001] The invention belongs to the technical field of biological materials, and relates to a method for preparing a halophilic bacteria photosensitive protein-titanium dioxide nanotube composite material. Background technique [0002] Bacillus rhodopsin, a photofunctional protein, is the simplest light-driven proton pump known to date. After bacillus rhodopsin absorbs photons, it quickly changes from a dark-adapted state to a light-adapted state, and the isomerization, protonation, and deprotonation of trans-retinal molecules occur. State K, L, M, N, O, and finally return to the initial state. During this cycle, bacillus rhodopsin releases protons outside the cell, and then takes up protons from inside the cell to establish a potential difference on both sides of the membrane, that is, photovoltage, and the photocurrent signal can be measured on the galvanometer. [0003] Bacteriorhodopsin can not only use light energy to synthesize adenosine triphosphate ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): H01L51/00H01L51/46B82Y30/00B82Y40/00C12P21/00C12R1/19
CPCY02E10/549
Inventor 杨丽娜李宇波杨杭生吴敏朱旭芬
Owner ZHEJIANG UNIV
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