Method for inducing production of tripterygium wilfordii hairy root by agrobacterium rhizogenes
A technology of Agrobacterium rhizogenes and hairy roots, which is applied in the field of using Agrobacterium rhizogenes to induce hairy roots of Tripterygium wilfordii
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[0015] A method of using Agrobacterium rhizogenes to induce hairy roots of Tripterygium wilfordii of the present invention, the specific content and implementation are as follows:
[0016] 1. Acquisition of aseptic explants of Tripterygium wilfordii: Pick leaves and tender stems of wild tripterygium wilfordii seedlings, rinse with clean water, cut into sections on an ultra-clean workbench, and scratch. Leaves cut into 1cm 2 , The tender stem is 2-4 cm long. Scratch the tender stems 2 to 3 times, and keep the cuts at both ends smooth. The explants were sterilized with 70% alcohol for 1 min, washed with sterile water for 3 times, and then soaked in 0.1% HgCl 2 After sterilizing in the solution for 10 minutes, rinse with sterile water for 3 times, dry the surface moisture with sterile filter paper, place in 1∕2MS liquid medium, and cultivate under 2000lx, 16h / d sunlight, and 25°C for 2-3 days. In order to make the wound site secrete more phenolic substances and improve the con...
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