Antiviral fusion protein and application thereof

A fusion protein, antiviral technology, applied in antiviral agents, peptide/protein components, hybrid peptides, etc., can solve the problems of high cost, difficult for patients to adhere to lifelong medication, and large side effects, and achieve good practical prospects.

Active Publication Date: 2015-05-27
南通九思医疗器械有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this therapy can delay the onset of AIDS, it has serious side effects and high cost, making it difficult for patients to adhere to life-long medication
There is no report that vMIP-II regulates the expression of host immune genes

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Antiviral fusion protein and application thereof
  • Antiviral fusion protein and application thereof
  • Antiviral fusion protein and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1. Protein preparation method

[0049] 1. Gene cloning to construct target protein expression vector

[0050] 1.1 Construction of pET-15b-PEP-1 plasmid

[0051] Two oligonucleotide chains of 21 amino acids of PEP1 (KETWWETWWTEWSQPKKKRKV) were chemically synthesized, and NdeI (CATATG) and XhoI (CTCGAG) restriction site sequences were added at both ends according to the characteristics of the selected pET-15b expression vector. The sequence is as follows:

[0052] Sequence 1:

[0053] 5'-NdelTATGAAAGAAACCTGGTGGGAAACCTGGTGGACCGAATGGTCTCAGCCGAAAAAAAAAACGTAAAGTGC-3'(68bp)

[0054] Sequence 2:

[0055] 3'-ACTTTCTTTGGACCACCCTTTTGGACCACCTGGCTTACCAGAGTCGGCTTTTTTTTTGCATTTCACGAGCT-5'Xhol(70bp)

[0056] Take out the two tubes of dry powder fragments, centrifuge at 3000rpm for 1min, mix the two fragments equimolarly with STE buffer (pH8.0), incubate at 94°C for 7min, turn off the water bath, let it slowly cool to room temperature for renaturation, and form the PEP-1 encoded DN...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides an antiviral fusion protein and application thereof. The fusion protein mainly comprises a cell penetrating peptide (CPP) structural domain and viral macrophage inflammatory protein (vMIP), wherein the cell penetrating peptide structural domain and the viral macrophage inflammatory protein form a serial structure. The fusion protein can enter cells in a non-receptor and non-energy-dependence mode, so that the antiviral immune gene expression level in the cells is effectively improved, finally, infection of viruses such as HIV (Human Immunodeficiency Virus), HBV (Hepatitis B Virus) and the like is suppressed, and a brand-new idea and a method are provided for prevention and treatment of acquired immune deficiency syndrome.

Description

(1) Technical field [0001] The invention relates to an antiviral fusion protein-cell penetrating peptide-viral macrophage inflammatory protein (CPP-vMIP) and its application in preparing anti-human immunodeficiency virus (humanimmunodeficiency virus, HIV) medicine. (2) Background technology [0002] Human immunodeficiency virus type 1 (HIV-1) is the pathogen of AIDS (AIDS), and HIV / AIDS is one of the most serious global public health problems facing mankind. Due to the highly mutated HIV-1 virus and HIV's direct attack on the host's immune system, there is still no effective prevention and treatment method, and no effective vaccine has been released yet. [0003] The use of reverse transcriptase inhibitors and protease inhibitors ("cocktail" therapy) in the treatment of AIDS has a strong effect of antagonizing virus proliferation. The "cocktail" therapy can temporarily reduce the level of virus in the body. Although this therapy can delay the onset of AIDS, it has great si...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00A61K38/16A61P31/18A61P1/16A61P31/20
Inventor 杨磊谭晓华王小波狄春红罗燕
Owner 南通九思医疗器械有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap