Dendrobium offcinale tissue cultured seedling training and domesticating method

Abstract

The invention provides a Dendrobium offcinale tissue cultured seedling training and domesticating method and aims at using a traditional Chinese medicine material culture and seedling raising technology to conduct three-month effective seedling training and domesticating management to Dendrobium offcinale tissue cultured seedlings in a seedling training greenhouse. Control and management are conducted mainly in six aspects, i.e. the design and the construction of the facilities of the seedling training greenhouse, the selection and the management of seedling training mediums, the management before the tissue cultured seedlings come out of bottles in the seedling training greenhouse, the treatment after the tissue cultured seedlings come out of the bottles in the seedling training greenhouse, the training and the field planting of the tissue cultured seedlings, and the management after the training and the field planting of the tissue cultured seedlings. The survival rate of the tissue cultured seedlings during seedling training is effectively improved to approximately 95 percent from the traditional 70 percent and the survival rate of the Dendrobium offcinale tissue cultured seedlings during seedling training is greatly improved. The seedlings which are trained and domesticated for three months can basically adapt to the growth environment conditions of the greenhouse, the leaves of plants obviously become thicker, the color becomes deeper, the stalks become stronger, the root systems become more robust, the survival rate of the domesticated seedlings after transplanting reaches more than 98 percent and the economic benefit and social benefit are produced very greatly.

Description

technical field [0001] The invention relates to a seedling hardening and domestication method of dendrobium candidum tissue culture seedlings, which belongs to the technical field of cultivation and seedling cultivation of traditional Chinese medicinal materials. Background technique [0002] Dendrobium officinale is one of the main varieties recorded in the "Chinese Pharmacopoeia". It has high medicinal value and strong health care function. The main components are polysaccharides, dendrobine, amino acids and trace elements. Eye, enhance human immunity, anti-aging, anti-tumor and other effects. Over the years, due to the destructive excavation by farmers in the production areas, the resources of wild Dendrobium candidum are on the verge of extinction. The state has listed Dendrobium candidum as a rare and endangered species under key protection, and encouraged the artificial cultivation of Dendrobium candidum in large areas, turning wild into domestic species, and Guarante...

AI Technical Summary

Problems solved by technology

The tissue culture technology of Dendrobium candidum is relatively mature and has developed rapidly; however, the artificial large-scale planting of Dendrobium candidum is still in its infancy in my country, and the scale is not large, and the speed of advancement is slow

One of the main reasons is that the transplanting survival rate of Dendrobium candidum tissue culture seedlings is low, especially during the period from January to March when the tissue culture seedlings come out of the bottle and go to the ground. Due to the large difference between the environmental conditions of tissue culture production and the growth conditions of transplanting, tissue culture The period of high incidence of seedling death severely restricts the scale and industrialization of Dendrobium officinale

In recent years, some scientific research institutes and enterprises have carried out research on the domestication technology of Dendrobium candidum tissue culture seedlings from January to March when they come out of the bottle and go to the ground. There are still some deficiencies in the control and management of key technologies, resulting in that the survival rate and yield of Dendrobium officinale tissue culture seedlings after transplanting are still not effectively guaranteed