Magnetic separation method of chlorella

Abstract

The invention discloses a magnetic separation method of chlorella and a recycling and regenerating method of a magnetic medium. The method comprises the following steps: (1) dispersing Fe3O4 nano-particles to form a stable suspension; (2) adding the suspension into a chlorella culture solution, stirring, and preparing a polymer of chlorella and magnetic medium in a magnetic separation mode; (3) cleaning the polymer, performing a reaction by adding hydrochloric acid, and performing microfiltration, cleaning and filtrate collection after the reaction is completed; and (4) regulating the pH value of the filtrate to be between 0.8 and 1.5 by using ammonia water, and preparing Fe3O4 nano-particles by taking the regulated filtrate as a raw material so as to realize regeneration and recycling ofthe magnetic medium. The medium used in the magnetic separation method provided by the invention has simple synthesizing steps and is easily amplified; the method of dissolving hydrochloric acid and recrystallizing ammonia water is adopted to realize regeneration and recycling of the Fe3O4 nano-particles, the chlorella components cannot be destroyed, and no pollution is generated; and the magnetic medium recycling process is simple and effective, and can be applied to industrial separation of the chlorella, so that the cost can be lowered.

Description

technical field [0001] The invention relates to a magnetic medium recovery method for magnetic separation of chlorella, which belongs to the field of separation of microalgae. Background technique [0002] Chlorella is a single-celled green algae, a eukaryotic organism, and its cells are rich in chlorophyll. Chlorella is rich in protein, vitamins, minerals, dietary fiber, nucleic acid and chlorophyll, especially it contains remarkable biologically active substances such as glycoproteins, polysaccharides and up to 13% of nucleic acids and other substances. [0003] Chlorella has the functions of enhancing human immunity, preventing virus proliferation, inhibiting cancer cell proliferation, inhibiting blood sugar rise, reducing serum cholesterol levels, eliminating toxins, and quickly repairing body damage. For example, in 1977, in the Journal of the Taiwan Medical Association, the report of the Taipei Medical College’s Biochemical Research Laboratory using chipmunks was publ...

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Problems solved by technology

Gao and Peng et al. (Gao Z.W., Peng X.J., Zhang H.M., Luan Z.K., Fan B.Desalination, 2009, 247, 337) successfully isolated harmful microalgae Microalgae aeruginosa from water using a composite of montmorillonite and copper-iron oxides. Cystic algae (Microcystis aeruginosa), its magnetic separation medium can be reused, but the synthesis of the magnetic complex is relatively cumbersome and requires a large amount; Liu and Li et al. (Liu D., Li F.T., Zhang B.R.Water Sci.Technol.2009, 59, 1085) used chitosan-modified magnetic polymer as the magnetic separation medium to successfully remove algae blooms in fresh water, but the synthesis of the magnetic separation medium was complicated, and the magnetic separation medium could not be reused and was greatly affected by the ionic strength.

[0013] It can be seen that the separation and collection of microalgae using existing magnetic materials requires complex material synthesis, and often requires modification or coating of polymers to couple with algae cells, and the recycling of magnetic materials requires high energy consumption. The calcination method is not conducive to the comprehensive utilization of microalgae, which greatly increases the cost and is difficult to apply on a large scale. Therefore, the selection of magnetic media and its recovery technology determine the use of magnetic separation technology in the field of microalgae separation and collection. One of the key issues for the successful application of

[0014] At the same time, the current magnetic material is only suitable for microalgae, and for chlorella, because its size, characteristics, etc. are different from microalgae, the current magnetic material is not suitable for chlorella

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